| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP006881 | 2,181,393 | C→T | 13.8% | P111P (CCG→CCA) | U712_10920 ← | Menaquinol‑cytochrome c reductase cytochrome b/c subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,181,393 | 0 | C | T | 13.8% | 76.8 / 4.0 | 29 | P111P (CCG→CCA) | U712_10920 | Menaquinol‑cytochrome c reductase cytochrome b/c subunit |
| Reads supporting (aligned to +/- strand): ref base C (13/12); new base T (2/2); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
CAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGT > CP006881/2181270‑2181500 | cAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGgcg < 1:235974/139‑1 (MQ=255) cagcGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAACAGCGAGTCCCGGCCTAATCATGGCGCCGCCCCCTg > 4:256569/1‑138 (MQ=255) cGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCGCTCGGTTCGTCAGGCAAGGAATGGCGCCAAAAGAAGGGCGCCAAAAGCGACTACCGGCATAATCATGGCGCCGACCACTGTa > 4:78982/1‑137 (MQ=255) cAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGCCCACTGTAAAGCTTCcggcg > 2:195629/1‑139 (MQ=255) aaGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGAc > 1:41862/1‑117 (MQ=255) aaGCATCATACCGACAGCGACCGGACGCTTCCACGGCCTTCTTTCTGTCCCTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGAc < 2:41862/117‑1 (MQ=255) atcatACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAACCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTAtt < 5:89452/139‑1 (MQ=255) catACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCa < 1:195629/139‑1 (MQ=255) cGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGtat > 5:110516/1‑138 (MQ=255) ttCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCCCTGTAAAGCTTCCGGTGGCGTATTCATACTTGAACAGCTGGttttag > 8:164222/1‑133 (MQ=255) ccACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATaagaa < 5:81071/138‑1 (MQ=255) ccTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGAc < 8:87914/81‑1 (MQ=255) ccTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGAc > 7:87914/1‑81 (MQ=255) ttCACTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCCGCGGCGTATTCATAATTAAACACCGTGTTTAAgaaaagaaaaa > 6:81071/1‑137 (MQ=255) ctctctGTTCCTATGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCATCCGGCGGCGTATTCATACATCAACACCTGGTATAAGAAAAGAAAAAAc > 4:176204/1‑138 (MQ=255) gtcccTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATAc < 4:286317/129‑1 (MQ=255) gtcccTCTGTCTAGGAATGGAGCCAAAAGCAGGGCCCCAAAAGCGAGACCTGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATAc > 3:286317/4‑132 (MQ=255) gTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTa > 5:97654/1‑78 (MQ=255) gTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTa < 6:97654/78‑1 (MQ=255) gTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATcct > 5:147294/1‑138 (MQ=255) ttCCTATGTAAAGGAATGGCGCCAAAAGCTGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCgg < 1:112293/139‑1 (MQ=255) ctctGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACa > 7:269033/1‑105 (MQ=255) ctctGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACa < 8:269033/105‑1 (MQ=255) tGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGTGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGc < 7:164222/137‑1 (MQ=255) tGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGc < 5:216154/137‑1 (MQ=255) aTGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAAcc < 3:176204/139‑1 (MQ=255) gcgcCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAata < 1:36438/131‑1 (MQ=255) gcgcCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAata > 2:36438/1‑131 (MQ=255) gcgcCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGGATTCCAACTTAAACAGCGGGTAAAAGAAAAGAAAATACCAAACCCGCAGCGGAATAAAACCTGt > 2:168500/1‑139 (MQ=255) | CAAAAATACAGCAGCGGAAATCGCCAAAAGCATCATACCAACAGCAACAGGACGCTTCCACGGCCTTCTCTCTGTTCCTCTGTCAAGGAATGGCGCCAAAAGCAGGGCGCCAAAAGCGAGTCCCGGCATAATCATGGCGCCGACCACTGTAAAGCTTCCGGCGGCGTATTCATACTTCAACAGCTGGTATAAGAAAAGAAAATACCAATCCGGCAGCGGAATATAACCTGT > CP006881/2181270‑2181500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |