Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,801,996	G→A	100%	A327T (GCC→ACC)	U712_14440 →	UPF0118 membrane protein ytvI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,801,996	0	G	A	100.0%	137.8 / NA	46	A327T (GCC→ACC)	U712_14440	UPF0118 membrane protein ytvI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/27);  total (19/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTA  >  CP006881/2801868‑2802111                                                                                                                                 |                                                                                                                    ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc                                                                                                           <  4:210005/139‑1 (MQ=255)   ccGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc                                                                                                           <  7:280514/136‑1 (MQ=255)                 ccAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTc                                                                                           <  8:3422/139‑1 (MQ=255)                   aaGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg                                                                                         <  6:68412/139‑1 (MQ=255)                               ttGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACt                                                                             <  2:104454/139‑1 (MQ=255)                                       cTGTACCTCGTCGTTCTTTTTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTAACGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCGTTAAATTATTAGGGGTTTTTGGACTGATTGCgg                                                                     >  6:292858/1‑139 (MQ=255)                                       cTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTc                                                                                           <  6:213572/117‑1 (MQ=255)                                       cTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTc                                                                                           >  5:213572/1‑117 (MQ=255)                                          tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCggg                                                                    <  6:24798/137‑1 (MQ=255)                                          tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                  <  2:195775/139‑1 (MQ=255)                                               cgtcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCt                                                             >  8:228172/1‑139 (MQ=255)                                               cgtcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCt                                                             >  4:265873/1‑139 (MQ=255)                                               cgtcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCt                                                             >  6:151149/1‑139 (MQ=255)                                                    ttCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACt                                                        <  3:265873/139‑1 (MQ=255)                                                         aTTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc                                                    <  5:23461/138‑1 (MQ=255)                                                          ttCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGTGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtcat                                                  <  1:306550/139‑1 (MQ=255)                                                               cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCatcatc                                              >  3:147049/1‑138 (MQ=255)                                                                    gCTGACAGAGACCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCt                                        <  1:244247/139‑1 (MQ=255)                                                                         cAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTATGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcat                                   <  5:227514/139‑1 (MQ=255)                                                                                  aGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAgg                          <  5:151149/139‑1 (MQ=255)                                                                                        tatgTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt                     >  1:1001/4‑138 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCAccc                                                                                                                  <  5:22895/45‑1 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCAccc                                                                                                                  >  6:22895/1‑45 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                  <  5:303600/93‑1 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                  <  3:186778/93‑1 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                  >  6:303600/1‑93 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc                                                                  >  4:186778/1‑93 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt                     >  5:265609/1‑138 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGG‑CTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcat                                   >  6:158865/1‑123 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGG‑CTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcat                                   <  5:158865/123‑1 (MQ=255)                                                                                        tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACACTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt                     >  7:260328/1‑138 (MQ=255)                                                                                               aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg               >  2:220248/1‑137 (MQ=255)                                                                                               aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAg               <  1:220248/137‑1 (MQ=255)                                                                                               aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGa              >  5:152625/1‑138 (MQ=255)                                                                                                  tATCTGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata          <  8:260328/139‑1 (MQ=255)                                                                                                    tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata          <  6:22651/137‑1 (MQ=255)                                                                                                    tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATg        >  6:29107/1‑139 (MQ=255)                                                                                                      gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg       <  5:292858/138‑1 (MQ=255)                                                                                                      gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg       <  7:228172/138‑1 (MQ=255)                                                                                                      gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg       <  6:83771/138‑1 (MQ=255)                                                                                                      gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg       <  4:147049/138‑1 (MQ=255)                                                                                                         atcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata          <  7:216872/132‑1 (MQ=255)                                                                                                         atcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata          >  8:216872/1‑132 (MQ=255)                                                                                                          tcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGCCCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCta  >  2:77043/1‑139 (MQ=255)                                                                                                              tcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATcc                                             <  7:8963/92‑1 (MQ=255)                                                                                                              tcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATcc                                             >  8:8963/1‑92 (MQ=255)                                                                                                                                 |                                                                                                                    TTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTA  >  CP006881/2801868‑2802111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑