Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,911,596 A→G 6.3% S317P (TCC→CCC)  U712_15005 ← L‑Lysine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,911,5960AG 6.3% 185.5 / 3.9 63S317P (TCC→CCC) U712_15005L‑Lysine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (27/32);  new base G (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

AGGAAGAGCGTGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCATCAGATCAGGCTGGA  >  CP006881/2911459‑2911733
                                                                                                                                         |                                                                                                                                         
aGGAAGAGCGTGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGaa                                                                                                                                          >  3:257486/1‑139 (MQ=255)
     gagCGTGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGcc                                                                                                                                     <  1:77811/139‑1 (MQ=255)
      agCGTGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCa                                                                                                                                    >  1:252797/1‑139 (MQ=255)
      agCGTGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCa                                                                                                                                    >  8:49368/1‑139 (MQ=255)
       gCGTGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCAt                                                                                                                                   >  1:241343/1‑139 (MQ=255)
       gCGTGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCAt                                                                                                                                   >  3:66649/1‑139 (MQ=255)
          tGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATACTGCCAGATTTTCAAGCGCAACCGCACAGCCATGCTGATTGCCTGTATAGGAATGGCCATgg                                                                                                                                 >  5:282528/1‑138 (MQ=255)
               aTCTTGAAGAAGAAAATGGAGCTTTTTAGTTTTTTCCGCTACTTGTTCCACATTGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGACAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAg                                                                                                                           >  5:34221/1‑139 (MQ=255)
                         agaaAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGGATGGCCATGGAAAAAGGTTTTTAGGt                                                                                                                 >  6:80165/1‑139 (MQ=255)
                         agaaAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGGATGGCCATGGAAAAAGGTTTTTAGGt                                                                                                                 >  6:84492/1‑139 (MQ=255)
                          gaaAATGGAGCTTTTTACTTTTTTCCTCTACTTTTTCCACAATGTTTTCAGATTCAATTAATGCCAGATTTTCACGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATCGGCATGGTAAAAGGTTTTTAGGtt                                                                                                                >  6:80008/1‑139 (MQ=255)
                          gaaAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGtt                                                                                                                >  4:266166/1‑139 (MQ=255)
                          gaaAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGtt                                                                                                                >  1:20246/1‑139 (MQ=255)
                          gaaAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGtt                                                                                                                >  5:53196/1‑139 (MQ=255)
                                    cTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAatca                                                                                                      <  7:224330/139‑1 (MQ=255)
                                     tttttACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAat                                                                                                        >  2:289534/1‑136 (MQ=255)
                                     tttttACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAat                                                                                                        <  1:289534/136‑1 (MQ=255)
                                           cTTTTTTGCGCTACTTGTTCCTCTATGTTTTCAGATTCAAATAATGCCAGATTATCAAGCGCAACCGCACAGCCAAGCAGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  1:112575/139‑1 (MQ=255)
                                           cTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGGATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  5:84492/139‑1 (MQ=255)
                                           cTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGGATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  5:80165/139‑1 (MQ=255)
                                           cTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  6:53196/139‑1 (MQ=255)
                                           cTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  2:20246/139‑1 (MQ=255)
                                           cTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  7:173714/139‑1 (MQ=255)
                                           cTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  4:257486/139‑1 (MQ=255)
                                           cTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  2:62749/139‑1 (MQ=255)
                                              ttttCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGaa                                                                                              <  3:286184/137‑1 (MQ=255)
                                              ttttCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATg                                                                                            >  6:7201/1‑139 (MQ=255)
                                              ttttCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTATCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGaa                                                                                              <  6:138684/137‑1 (MQ=255)
                                               tttCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATg                                                                                            <  2:212842/138‑1 (MQ=255)
                                                        cTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTGTAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGAt                                                                                  <  8:263987/139‑1 (MQ=255)
                                                        cTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGAt                                                                                  <  8:151845/139‑1 (MQ=255)
                                                        cTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGAt                                                                                  <  8:237655/139‑1 (MQ=255)
                                                              ccACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCTCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTATGTTTTCATAATCATCATAGAATGCCTTATAGATGTCtt                                                                             <  1:43464/138‑1 (MQ=255)
                                                               cacaATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCa                                                                           <  3:266166/139‑1 (MQ=255)
                                                                  aaTGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTg                                                                        >  2:141548/1‑139 (MQ=255)
                                                                    tGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCATTATAGATGTCTTCAGTgg                                                                       >  6:290028/1‑138 (MQ=255)
                                                                    ggTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAAACGCACAGCAAAGCTGATTGCCTGTATAGTACTGTCCATTGATATAGGTTTTTAGGTTTTCATAATCATCATTGAATGCCTTATATCTGTCTTCAGTGGc                                                                      <  4:75933/138‑1 (MQ=255)
                                                                         tCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACg                                                                 >  3:299903/1‑139 (MQ=255)
                                                                         tCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACg                                                                 >  3:229601/1‑139 (MQ=255)
                                                                            gATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTaa                                                              <  5:116926/139‑1 (MQ=255)
                                                                                       tGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCa                                                                                                                                    >  3:195712/1‑58 (MQ=255)
                                                                                       tGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCa                                                                                                                                    <  4:195712/58‑1 (MQ=255)
                                                                                       tGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               >  7:190840/1‑95 (MQ=255)
                                                                                       tGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGa                                                                                               <  8:190840/95‑1 (MQ=255)
                                                                                       tGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTgg                                                    >  7:83941/1‑138 (MQ=255)
                                                                                            gATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATa                                              <  6:152639/139‑1 (MQ=255)
                                                                                                 tccaGCGCAACCGGACAGCCATGCTGATTGCCTGTATAGGAATGGCCAAGGAAAAACGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGcctc                                         <  6:34221/136‑1 (MQ=255)
                                                                                                      cgcAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTa                                    <  1:141548/139‑1 (MQ=255)
                                                                                                         aaCCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATg                                 <  6:18862/139‑1 (MQ=255)
                                                                                                                   ccAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCgg                       >  6:201251/1‑139 (MQ=255)
                                                                                                                      aGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGt                                     <  6:170716/122‑1 (MQ=255)
                                                                                                                      aGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGt                                     >  5:170716/1‑122 (MQ=255)
                                                                                                                           aTTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCat                >  3:190770/1‑138 (MQ=255)
                                                                                                                           aTTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCa                 <  5:111679/137‑1 (MQ=255)
                                                                                                                           aTTGCCTGTATAGGAATGGCCATGGAAAAAGGGTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCa                 <  7:94602/137‑1 (MQ=255)
                                                                                                                             tGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCat                >  4:209419/1‑136 (MQ=255)
                                                                                                                             tGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCat                >  3:81022/1‑136 (MQ=255)
                                                                                                                               ccTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCatcaga            <  5:201251/138‑1 (MQ=255)
                                                                                                                               ccTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCatcaga            <  4:66649/138‑1 (MQ=255)
                                                                                                                                  gTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGcc                                           <  7:187945/104‑1 (MQ=255)
                                                                                                                                  gTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGcc                                           >  8:187945/1‑104 (MQ=255)
                                                                                                                                  gTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCa                 <  7:83192/130‑1 (MQ=255)
                                                                                                                                  gTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCa                 >  8:83192/1‑130 (MQ=255)
                                                                                                                                   tataGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCATCAGATCAgg       >  5:169292/1‑139 (MQ=255)
                                                                                                                                         aaTGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCATCAGATCAGGCTGGa  >  6:238387/1‑138 (MQ=255)
                                                                                                                                         aaTGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCATCAGATCAGGCTGGa  >  1:75914/1‑138 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AGGAAGAGCGTGCAGATCTTGAAGAAGAAAATGGAGCTTTTTACTTTTTTCCGCTACTTGTTCCACAATGTTTTCAGATTCAAATAATGCCAGATTTTCAAGCGCAACCGCACAGCCAAGCTGATTGCCTGTATAGGAATGGCCATGGAAAAAGGTTTTTAGGTTTTCATAATCATCATAGAATGCCTTATAGATGTCTTCAGTGGCAAACGTAACGGCAATTGGCAAATAGCCTCCTGTAATGCCTTTACCGGCAGCCATCAGATCAGGCTGGA  >  CP006881/2911459‑2911733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑