Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,175,196 T→A 7.7% N38I (AAT→ATT)  U712_16305 ← NifU‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,175,1960TA 7.7% 181.9 / 3.0 61N38I (AAT→ATT) U712_16305NifU‑like protein
Reads supporting (aligned to +/- strand):  ref base T (25/31);  new base A (3/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCCCTTTAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTGCATTAAAAGACATTTGTAAAATACTCCTTTGTCTTTT  >  CP006881/3175060‑3175332
                                                                                                                                        |                                                                                                                                        
tttCCCTTTAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTg                                                                                                                                        >  1:30750/1‑139 (MQ=255)
tttCCCTTTAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTg                                                                                                                                        >  2:34880/1‑139 (MQ=255)
  tCCCTTTAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGtt                                                                                                                                      >  3:124064/1‑139 (MQ=255)
        tAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTTTTCATATc                                                                                                                                >  3:10325/1‑139 (MQ=255)
        tAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTc                                                                                                                                     <  3:29175/134‑1 (MQ=255)
        tAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTc                                                                                                                                     >  4:29175/1‑134 (MQ=255)
        tAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATc                                                                                                                                >  1:89222/1‑139 (MQ=255)
        tAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATc                                                                                                                                >  3:135292/1‑139 (MQ=255)
         aaTCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCTCATGTCGGATTGTTCATATc                                                                                                                                <  2:30750/138‑1 (MQ=255)
         aaTCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATc                                                                                                                                <  5:284500/138‑1 (MQ=255)
         aaTCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATc                                                                                                                                <  4:296083/138‑1 (MQ=255)
         aaTCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATc                                                                                                                                <  3:78998/138‑1 (MQ=255)
         aaTCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCACTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATc                                                                                                                                <  1:33792/138‑1 (MQ=255)
          aTCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCa                                                                                                                              <  1:97705/139‑1 (MQ=255)
              ccTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCAGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCCGAATGTTCATATcaaaca                                                                                                                           >  4:24312/1‑133 (MQ=255)
                      atcatcGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGt                                                                                                                  <  3:69790/139‑1 (MQ=255)
                      atcaacGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCAGCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTg                                                                                                                   <  3:225521/138‑1 (MQ=255)
                       tcatcGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGt                                                                                                                  >  8:122601/1‑138 (MQ=255)
                        catcGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCa                                                                                                                >  6:257827/1‑139 (MQ=255)
                             aaGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGTATTGTTCATATCCACGACAATGCTGTCATTTaa                                                                                                           <  1:298181/139‑1 (MQ=255)
                             aaGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTa                                                                                                            >  8:33079/1‑138 (MQ=255)
                               gCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTaaaa                                                                                                         <  3:24312/139‑1 (MQ=255)
                                              aTGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGGGGTCGCCGCATGTCGGATTGTTAATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTcgcg                                                                                          <  2:90112/139‑1 (MQ=255)
                                                 gAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGt                                                                                       >  7:157474/1‑139 (MQ=255)
                                                 gAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCGTTATTTCGCGGGt                                                                                       >  1:287486/1‑139 (MQ=255)
                                                       ccTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTg                                                                                  >  6:286824/1‑138 (MQ=255)
                                                        cTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTa                                                                                <  8:157474/139‑1 (MQ=255)
                                                        cTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTAATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTa                                                                                <  7:122601/139‑1 (MQ=255)
                                                           ccccTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTaa                                                                               <  2:42306/137‑1 (MQ=255)
                                                            cccTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATg                                                                             <  4:141901/138‑1 (MQ=255)
                                                             ccTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGGCAGTCTGATGCGGTCGACGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGAt                                                                           <  4:218052/139‑1 (MQ=255)
                                                             ccTTCAAACTTCGCAACTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGAt                                                                           <  5:176613/139‑1 (MQ=255)
                                                                 cAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCa                                                                        >  2:50449/1‑138 (MQ=255)
                                                                 cAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGAGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATTCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCa                                                                        >  5:277819/1‑138 (MQ=255)
                                                                    aCTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAAt                                                                    <  4:124064/139‑1 (MQ=255)
                                                                      ttCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCa                                                                  >  6:143301/1‑139 (MQ=255)
                                                                      ttCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCa                                                                  >  2:208173/1‑139 (MQ=255)
                                                                       tCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCa                                                                  <  4:10325/138‑1 (MQ=255)
                                                                        cGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCa                                                                  <  3:141699/137‑1 (MQ=255)
                                                                        cGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCa                                                                  <  1:50449/137‑1 (MQ=255)
                                                                        cGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCa                                                                  <  4:167999/137‑1 (MQ=255)
                                                                        cGCATCTTCACCTATGTCTCCGTCAAGCGTCATTGTCAGTCTGCTGAGCGCGCCGCATGTCTGATTTTTCATATCCAAGACAATGCTGTGATTTAAAAGCACTTTATTTCGCGGGTATTTGTAATGATCCATAAtaa                                                                  <  2:64252/137‑3 (MQ=255)
                                                                         gCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCAcc                                                                <  2:89222/138‑1 (MQ=255)
                                                                                         ctcCGTCAAGCTTCTTTGACAGTCTTATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATc                                               <  6:81667/139‑1 (MQ=255)
                                                                                          tcCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATc                                               >  7:157314/1‑138 (MQ=255)
                                                                                          tcCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGAATGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACctgt                                                             >  3:68034/1‑124 (MQ=255)
                                                                                          tcCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGAATGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACctgt                                                             <  4:72439/124‑1 (MQ=255)
                                                                                          tcCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGAATGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACctgt                                                             <  4:68034/124‑1 (MQ=255)
                                                                                          tcCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGAATGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACctgt                                                             >  3:72439/1‑124 (MQ=255)
                                                                                          tcCGTCAAGCTTCATTGACAGTCTGATGCGGTCGCCGCATGGCGGATTGTTAATATCCACGACAATTCTGTCATTTAAAACCCCATTATTTCGCGGGTTTTTGTTATGATCCATAATCACCTTTCAGTACAATTTAta                                               >  5:81667/1‑137 (MQ=255)
                                                                                               cAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGtt                                         <  5:257827/139‑1 (MQ=255)
                                                                                                aaGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGttt                                        >  3:302312/1‑139 (MQ=255)
                                                                                                  gCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACa                                                      >  7:297066/1‑123 (MQ=255)
                                                                                                  gCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACa                                                      <  8:297066/123‑1 (MQ=255)
                                                                                                  gCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTg                                       >  7:292125/1‑138 (MQ=255)
                                                                                                              gTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTGCATTAAAAGACAt                          <  3:136970/139‑1 (MQ=255)
                                                                                                              gTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTGCATTAAAAGACAt                          >  8:189777/1‑139 (MQ=255)
                                                                                                                     gCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTGCATTAAAAGACATTTGTaa                    >  5:86525/1‑138 (MQ=255)
                                                                                                                        gTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTGCATTAAAAGACATTTGTAAAATa                <  1:34880/139‑1 (MQ=255)
                                                                                                                               aaTGTCGGATTGTGCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGAGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTGCATTAAAAGACATTTGTAAAATACTCCttt         <  6:277819/138‑1 (MQ=255)
                                                                                                                                       aTTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTGCATTAAAAGACATTTGTAAAATAGTCCTTTTTCtttt  >  7:142576/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTTCCCTTTAATCGCCTGAGTCATCATCGAAGCGGATGCCATTGAAATGGAACAGCCTTCCCCTTCAAACTTCGCATCTTCCACGATGTCTCCGTCAAGCTTCATTGTCAGTCTGATGCGGTCGCCGCATGTCGGATTGTTCATATCCACGACAATGCTGTCATTTAAAACCCCTTTATTTCGCGGGTTTTTGTAATGATCCATAATCACCTGTCTGTACAATGTATCTAAGTTTGCATTAAAAGACATTTGTAAAATACTCCTTTGTCTTTT  >  CP006881/3175060‑3175332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑