Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,437,392 A→C 13.0% D192A (GAC→GCC) ‡ U712_17650 → putative MFS‑type transporter yvkA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,437,3920AC13.0% 268.7 / 3.8 138D192A (GAC→GCC) ‡U712_17650putative MFS‑type transporter yvkA
Reads supporting (aligned to +/- strand):  ref base A (70/47);  new base C (3/18);  total (73/65)
Fisher's exact test for biased strand distribution p-value = 1.09e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

CACTCGGGCCTTTTATCGGCTCTATACTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACAGTGTATTGATACTTCTTTTTGTTCCGCT  >  CP006881/3437254‑3437521
                                                                                                                                           |                                                                                                                                 
    cGGGCCTTTTTTGGGCTATATACTTATTGACCTCTTAAGGTGGCATTCGATTTTTGGGGTAAACATCCCGTTCCTCGCCTTTGCTTTATTTACGCCATT‑ACCGATTTTTCCTAAATATAAACAAAAAAAATCTCAcgcg                                                                                                                               <  2:113975/139‑1 (MQ=255)
     gggCCTTTTATCGGCTCTATACTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTTTTTACGGCTTT‑AAGGATGGTTCGGCTATAGAAAGAAGATAAAGCTGCCgcgc                                                                                                                              >  2:207613/1‑139 (MQ=255)
     gggCCTTTTATCGGCTCTATACTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACgcgc                                                                                                                              >  3:223129/1‑139 (MQ=255)
       gCCTTTTATCGGCTCTATACTTATTGACAGCTTAAGCTGGCATTCTATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATA‑ACCGATGTTTCCTCAATAAAAACAAAATAAATCTCCCGcgccg                                                                                                                            <  6:215067/139‑1 (MQ=255)
             tATCGGCTCTATACTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGa                                                                                                                       >  1:40389/1‑138 (MQ=255)
                   ctctATACTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATatcatc                                                                                                                >  2:72366/1‑139 (MQ=255)
                     ctATACTGATTTATAGATTCAGTTGTATTTCTTTTTTTTGGGACAACATCCGGTTCCTCTCCATTGATTTATTTACGGCTTA‑AAGGTTGTTTCATAATTATAAATAAAAAAATTCTCCCGCGCCGCTGGATATCATCg                                                                                                               <  2:165660/138‑1 (MQ=255)
                      tataCTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAATGAAAATAAATCTGACGCGCCGCTGGATATCATCGGc                                                                                                             >  1:256415/1‑139 (MQ=255)
                      tataCTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTATCCTCAATATAAAGAAAATAAATATGCCGCGCCGCTGGATATCATCGGc                                                                                                             >  5:45389/1‑139 (MQ=255)
                      tataCTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGAATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGc                                                                                                             <  1:72366/139‑1 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGTCATT‑AACTATGTTTCCTCAATATAAAGAAAATAAATCTCACGCGCCGCTGGATATCATCGGctctctat                                                                                                      >  2:151334/1‑137 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGCCGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  6:67312/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  8:218286/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  7:221561/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  6:232665/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  3:114886/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  3:167618/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  6:284180/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  3:277867/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  8:197195/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  3:291268/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  7:69941/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  7:56460/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  4:208525/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  4:259241/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  4:308285/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  8:305922/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  1:189252/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  2:28190/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  1:305205/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  1:51326/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  1:56230/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTGTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  4:12798/1‑139 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGATTTTATGGGTCAACATCCCGATCCTCCCCATACCTATATTTACGGCATT‑AACGATGTTTCCACAATATAAAGAAAATAAAACTGACGCGCCGCTGGATATCATCGGctctctat                                                                                                      >  2:181774/1‑137 (MQ=255)
                             aTTGACAGCTTCAGCTGGCATTCGAGTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGCAAATAAATCTGACGCGCCGCTGGATATCATCGGctctctct                                                                                                      >  6:217424/1‑139 (MQ=255)
                             aTTGACAGCTTCAGATGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTAGCCATAGCTTTATTTACTTCAGT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTCAAGCGCCGCTGGATATCATCGCatctctct                                                                                                      >  2:151671/1‑139 (MQ=255)
                              ttGACAGCTTCAGGTGGCATTCTATTTTTTGGGTAAACATCCCGTACCTCGCCATTGATTTATTTACGGAATA‑AACGATGTTTCCTCAATATAAATAAAATAAATCTCACGCGCCGCTGGATATCATCGGctctctct                                                                                                      <  4:302303/138‑1 (MQ=255)
                                gACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGcgccg                                                                                                                            >  8:239002/1‑114 (MQ=255)
                                gACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGcgccg                                                                                                                            <  7:239002/114‑1 (MQ=255)
                                gACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTcttgc                                                                                                   >  6:111180/1‑139 (MQ=255)
                                gACAGCTTCAGCTGGCATTCGATTATTT‑GGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAATTCTGACGCGCCGCTGGATAGCATCGGCTCTCTcttgct                                                                                                  >  1:32960/1‑139 (MQ=255)
                                  cAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTcttgctt                                                                                                 >  1:156047/1‑139 (MQ=255)
                                  cAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTcttgctt                                                                                                 >  2:128058/1‑139 (MQ=255)
                                  cAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTcttgctt                                                                                                 >  8:265553/1‑139 (MQ=255)
                                    gCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCttgcttgc                                                                                               <  7:218286/139‑1 (MQ=255)
                                               cATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCGTT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCg                                                                                    >  5:130130/1‑139 (MQ=255)
                                                attgtaTTTTTTGCGTCAACAACCCGTGCCGCGCAATTGCTTTATTTACGTCATT‑CAGGTTTTTTCCAAAATAAAAACAAAATAAATCTGCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGt                                                                                   <  5:221646/134‑1 (MQ=255)
                                                         tttGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTa                                                                           >  1:145803/1‑138 (MQ=255)
                                                                            ccTCGCAATTTTTTTATTTTCGGAATT‑AACGTTGTTTCATAAATATAAATAAAATAAATGTGACGCGCCGCTGGATATCATCGTCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGa                                                        <  8:237825/138‑1 (MQ=255)
                                                                             cTCGCCTTTGCTTTTTTTACGGTATT‑AACGATGTTTCCTAAATAAAAAGAAAATAAATTTCACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAgg                                                      <  3:308285/139‑1 (MQ=255)
                                                                             cTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAgg                                                      >  6:196090/1‑139 (MQ=255)
                                                                               cGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCt                                                    >  4:239432/1‑139 (MQ=255)
                                                                               cGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCt                                                    >  8:65813/1‑139 (MQ=255)
                                                                                  cATTGTTTTATTTACGGAATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCa                                                 <  2:209221/139‑1 (MQ=255)
                                                                                  cATTGCTTTATTTCCGGAATT‑AAGGATGTTTCAGCAATATAAAGAAAAAAAAGCTGCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCa                                                 <  7:197195/139‑1 (MQ=255)
                                                                                  cATTGCTTTATTTACGGCTTT‑AACGATGTTTCCTCAATATAAATAAAATAAATTTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCa                                                 <  8:11717/139‑1 (MQ=255)
                                                                                  cATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCa                                                 <  4:277867/139‑1 (MQ=255)
                                                                                  cATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCa                                                 <  3:239432/139‑1 (MQ=255)
                                                                                  cATTGCTTTATTTACGGAATT‑AACGATGTTTCATAAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCa                                                 <  2:40389/139‑1 (MQ=255)
                                                                                  cATTGCTTTATTAACGGAATT‑AACGATGTTTCCTCAATATAAATAAAATAAATCTCCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCa                                                 <  3:48371/139‑1 (MQ=255)
                                                                                  cATTGATTTATTTACGTAATT‑AACGATGTTTCCTAAATATAATCAAAATATATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCTTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCa                                                 <  6:188230/139‑1 (MQ=255)
                                                                                     tGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATgg                                              >  5:164112/1‑139 (MQ=255)
                                                                                     tGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCATGCTGACGAAAAATGAGGCTCCATgg                                              >  7:242115/1‑139 (MQ=255)
                                                                                       cTTTATTTAAGGGATT‑AACGATGTTTAATCAATATAAATAAAATAAATCTTACGCTCCGCTGGATATCATCGGCTATCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATgggg                                            <  2:32960/139‑1 (MQ=255)
                                                                                          tttttaCGGCTTT‑AACGATGTTTCCTAAATATAAAGCAAATAAATCAGCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAAAGAGGCGCCATGGGGat                                          <  8:304399/136‑1 (MQ=255)
                                                                                          tttttaCGCCATT‑AAGTTTGTTTCCTCAATAAAAATAAAATAAATGTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  1:238540/136‑1 (MQ=255)
                                                                                          tctttaCGGCATA‑AACGATTTTTCATAAAAAAAAAGAAAAAAAATCTGCCGCGCCGCTGGATATCATCGGCTCTCTCTTTCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGTCTCCATGGGGat                                          <  6:301988/136‑1 (MQ=255)
                                                                                          tatttcCGGAATT‑AACTATGTTTCACAAATAAAAATAAAATAAATCCGCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  1:127344/138‑1 (MQ=255)
                                                                                          tatttaCGGCTTA‑ACCGATGTTTCCTCAAAATAAAGAAAATAAATCTCACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  3:154747/138‑1 (MQ=255)
                                                                                          tatttaCGGCATT‑AACGTTGTTTCCTAAATATAAAGAAAATAAATCTGCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  8:221561/138‑1 (MQ=255)
                                                                                          tatttaCGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  5:284180/138‑1 (MQ=255)
                                                                                          tatttaCGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  5:111180/138‑1 (MQ=255)
                                                                                          tatttaCGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  1:19118/138‑1 (MQ=255)
                                                                                          tatttaCGGCATT‑AACGATGTTTCCTAAATATAACGAAAATAAATCTGCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  8:69941/138‑1 (MQ=255)
                                                                                          tatttaCGGCATT‑AACGATGTTTCCTAAATATAAATAAAATAAAGCTGCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  5:198114/138‑1 (MQ=255)
                                                                                          tatttaCGGCATT‑AACGATGTTTCCTAAATATAAACAAAATAAATCGCCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  1:161396/138‑1 (MQ=255)
                                                                                          tatttaCGGCATT‑AACGATGTTACATAAATAAAAACAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  3:116244/138‑1 (MQ=255)
                                                                                          tatttaCGGAATT‑CACGATGTTTCCTCAATAAAAAGAAAATAAATATGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  2:189252/138‑1 (MQ=255)
                                                                                          tatttaCGGAATT‑AAGGTTGTTTCTAAAATATAAAGAAAATAAATCTCCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  4:77266/138‑1 (MQ=255)
                                                                                          tatttaCGCCATA‑ACCCATGTTCCCTCAATAAAAATAAAATAAAACTCACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  5:232665/138‑1 (MQ=255)
                                                                                          aatttaCGCCATT‑ACCGTTGTTTCCACAATAAAAATAAAAAAAATCTGCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  4:177127/137‑1 (MQ=255)
                                                                                           tttttCGGGATTAAACTATTTTTCAACATTATAATCAAAATAATTCTACCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  1:128058/137‑1 (MQ=255)
                                                                                            tttaCGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGAtata                                       <  2:51326/139‑1 (MQ=255)
                                                                                             ttaCGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGAtata                                       <  7:305922/138‑1 (MQ=255)
                                                                                             ttaCGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGAtata                                       <  2:56230/138‑1 (MQ=255)
                                                                                                gcgCATT‑AACGATGTTTCCACAATAAAAAGAAAAAAAATCTCCCGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGt                                   <  4:114886/137‑1 (MQ=255)
                                                                                                 ggCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATAcctgt                                  >  1:162343/1‑135 (MQ=255)
                                                                                                 ggCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGta                                  >  5:219657/1‑139 (MQ=255)
                                                                                                     tt‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACa                              <  6:164112/139‑1 (MQ=255)
                                                                                                      taaaCGATGTTTCCTCAATAAAAACAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACa                              <  3:234060/137‑1 (MQ=255)
                                                                                                      t‑AACGATTTTTCATCAATATAAAGAAATTAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATAc                               <  6:130130/137‑1 (MQ=255)
                                                                                                      t‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACAg                             >  1:251029/1‑139 (MQ=255)
                                                                                                        aaCGTTGTTACATAAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACAg                             <  5:52858/138‑1 (MQ=255)
                                                                                                                       atatAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACAGTGTATTGAGActtctt             >  4:144043/1‑139 (MQ=255)
                                                                                                                                  ataaatCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATgggg                                            <  8:180479/97‑1 (MQ=255)
                                                                                                                                  ataaatCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATgggg                                            >  7:180479/1‑97 (MQ=255)
                                                                                                                                  ataaatCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGat                                          <  7:23079/99‑1 (MQ=255)
                                                                                                                                  ataaatCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGtata                                >  6:234729/1‑109 (MQ=255)
                                                                                                                                  ataaatCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACAGTGTATTGATACTTCTTTTTGTTCCGCt  >  3:285400/1‑139 (MQ=255)
                                                                                                                                  ataaatCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACAGTGTATTGATACTTCTTTTTGTTCCGCt  >  6:232349/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                                 
CACTCGGGCCTTTTATCGGCTCTATACTGATTGACAGCTTCAGCTGGCATTCGATTTTTTGGGTCAACATCCCGTTCCTCGCCATTGCTTTATTTACGGCATT‑AACGATGTTTCCTCAATATAAAGAAAATAAATCTGACGCGCCGCTGGATATCATCGGCTCTCTCTTGCTTGCAGGCAGCATCGTTTCCATTATCCTGCTGACGAAAAATGAGGCTCCATGGGGATATACTGTATACAGTGTATTGATACTTCTTTTTGTTCCGCT  >  CP006881/3437254‑3437521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑