Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,444,703 A→C 19.7% V43G (GTC→GGC)  U712_17685 ← UPF0750 membrane protein yvjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,444,7030AC19.7% 72.7 / 2.3 52V43G (GTC→GGC) U712_17685UPF0750 membrane protein yvjA
Reads supporting (aligned to +/- strand):  ref base A (29/12);  new base C (3/8);  total (32/20)
Fisher's exact test for biased strand distribution p-value = 1.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCAAGCGTCTTTAGTCCAAATTTCCCGCCGAGCAAAATGACGCCAGCAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCT  >  CP006881/3444571‑3444837
                                                                                                                                    |                                                                                                                                      
ccTGCAAGCGTCTTTAGTCCAAATTTCCCGCCGAGCAAAATGACGCCAGCAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCccgc                                                                                                                                  <  1:10821/139‑1 (MQ=255)
            tttAGTCCAAATTTCCCGCCGAGCAAAATGACGCCAGCAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCt                                                                                                                      >  1:292900/1‑139 (MQ=255)
                            cgccgAGCAAAATGACGCCAGCAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCaaaaa                                                                                                      <  7:55180/139‑1 (MQ=255)
                                 aGCAAAATGACGCCAGCAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGCCCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACAt                                                                                                 >  3:109447/1‑139 (MQ=255)
                                   cAAAATGACCCCATCAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTa                                                                                               <  3:175506/139‑1 (MQ=255)
                                                gCAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCg                                                                                  >  1:66558/1‑139 (MQ=255)
                                                 cAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCg                                                                                  >  2:275675/1‑138 (MQ=255)
                                                    taaataatTGTATATTAAAAATCCACTGTACATACGCTGCTTCAAAACCATTTTATTTTAAAATCGTACTGATCCCGCTCACCCCGCCGGCGGAAATATTATTCGCAACAAAAAATAAATTAACGCACACGGCCGTAAt                                                                              <  2:37127/139‑1 (MQ=255)
                                                      aataaaGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCCATCTTATTCGGAAGCAAAAATACATTAAATGACCCGGCCGTAATCg                                                                            >  2:139993/1‑139 (MQ=255)
                                                           aGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCcgg                                                                                                                               >  8:286506/1‑83 (MQ=255)
                                                           aGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCcgg                                                                                                                               <  7:286506/83‑1 (MQ=255)
                                                           aGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTa                                                                                               <  7:62396/115‑1 (MQ=255)
                                                           aGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTa                                                                                               >  8:62396/1‑115 (MQ=255)
                                                           aGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAAATCCTTAAAGGACACGGCCGTAATCggagcg                                                                       >  2:271353/1‑134 (MQ=255)
                                                           aGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAAACTTATACGGAAGCAAAAATACATTAAAGGACACGGCCGTAATcccagag                                                                       >  1:272089/1‑133 (MQ=255)
                                                               atatTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGAc                                                                                                                                       >  1:229157/1‑71 (MQ=255)
                                                               atatTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGAc                                                                                                                                       <  2:229157/71‑1 (MQ=255)
                                                               atatTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTa                                                                   >  4:123732/1‑139 (MQ=255)
                                                               atatAAAAAACCCACTGTACATCCGATGAAAAAAAACAATATTTTTTTAAATTCTTACTGCCCCCGCTCCCCCCGCCGGCGTAATTTTTATTCGCAAAAAAAAAAAAATAAACGCCCACGGCCGTAATCGCTGCTCCt                                                                    <  2:66558/138‑1 (MQ=255)
                                                                tatTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTAt                                                                  >  7:172386/1‑139 (MQ=255)
                                                                  ttAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTTAAGGACACGGCCGTAATCGCTGCTCCTATc                                                                 >  8:230284/1‑138 (MQ=255)
                                                                        aaccACTGTACATACGCTGATAAAAAACAATATTATTTTAAAATCGAACTCACCCCGCGGCCCCCGCCGGCGGCAATATTATTCGTAACAAAAAATACATAAACGGACACGGCCGTAATCGCTGCTCCTATCAATATGt                                                          <  2:170410/137‑1 (MQ=255)
                                                                         tCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGta                                                         >  8:299279/1‑139 (MQ=255)
                                                                              tGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGCCCCCGCAGGCGGCAATCTTATACGGAAACAAAAATACATTAAAGTACACGTCCGTAATAGCTGATCATATAAATATGtataaa                                                     >  4:210866/1‑136 (MQ=255)
                                                                              tGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACa                                                     >  8:302938/1‑138 (MQ=255)
                                                                                tACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCTTAATCGCTGCTCCTATCAATATGTATACATaa                                                  >  8:14572/1‑139 (MQ=255)
                                                                                     aCGCTGCTTCACAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGACGGCGGCAATATTAGTCGGAATAAAAAATACATTAAAGGACCCGGCAGTAATAGCTGCTCCTATCAATATGTATACATAATCCCg                                             >  7:198750/1‑139 (MQ=255)
                                                                                     aCGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCg                                             >  6:98357/1‑139 (MQ=255)
                                                                                         tgctTCAAACCCATATGATTGTAAAATCGTACTGATCCCCCTGCCCCCGCCGGCGGCAATCTTATGCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaa                                          >  2:13522/1‑138 (MQ=255)
                                                                                          gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCcggcgg                                                                                                                            >  4:114875/1‑55 (MQ=255)
                                                                                          gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCcggcgg                                                                                                                            <  3:114875/55‑1 (MQ=255)
                                                                                          gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCTGCGGCAAACATATTCGGAACCAAAAAAACATTAAAGGACACGGCCGTAATCCCTGCTCCTAACAATATGTAAACATAATACCCCaaa                                         >  1:115872/1‑138 (MQ=255)
                                                                                          gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                        >  1:53212/1‑139 (MQ=255)
                                                                                          gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                        >  8:92549/1‑139 (MQ=255)
                                                                                          gctTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCAGCTCCTATCAATATGTATACATAATCCCGCaaaa                                        >  5:18258/1‑139 (MQ=255)
                                                                                          gctTCAAAACCATATGATTGTAAAATCGTACTGATCCAGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaaa                                        >  7:243720/1‑139 (MQ=255)
                                                                                          gctTAAAAACCATATGATTGTAAAATCCTACTGATCCCGCTCACCCCGCCGGCGGCAATCTTAATCGGAAGCAAAAAAACATTAAAGGACACCGCAGTAAAAGCTGCTCCAATCAATATGTAAACATAATAACGCaaa                                         >  3:207266/1‑138 (MQ=255)
                                                                                           ctTAAAAACAATATTATTTAAAAATCGTACTGATCCCGCTCCCCCCGCCGGGGTAAATATTATTCGTAACAAAAAATAAATAAACCCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCaaa                                         <  5:278723/137‑1 (MQ=255)
                                                                                             tCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATc                                      >  7:287943/1‑138 (MQ=255)
                                                                                              cacagccATATGATTTAAAACTCTTACTCACCCCGCTCACCCCGCCGGCGGAATTCTTATTCGCAACAAAAAAAACATAAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCca                                    <  6:231872/134‑1 (MQ=255)
                                                                                                aaaCCATTTGTTTTTAAAAGCGCACCGAGCCCGCGGCCCCCGCCGGCGGAAATCTTATGCGCAAGAAAAAAAAAAAAACCGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                  <  2:11592/139‑1 (MQ=255)
                                                                                                aaaCCATATTATTTTAAAAGCGTACTGATCCCGCTGACCCCGCCGGCGTAAATATTATTCGCAACCAAAAAAACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                  <  5:138138/139‑1 (MQ=255)
                                                                                                aaaCAATTTTATTGTAAAATCGTACTCATCCCGCAGACCCCGCCGGCGTAAATCTTTTTCTTAACAAAAAATACATTAACCGCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                  <  2:292900/139‑1 (MQ=255)
                                                                                                aaaCAATATTTTTTTATAATGGTAGTGACCCCTGAGCCCCCGCCGGCGCAATTTTTATTCGTAATAAAAAATACATTAAACCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCcaca                                  <  7:299279/139‑1 (MQ=255)
                                                                                                      tatGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGttt                            >  5:300498/1‑139 (MQ=255)
                                                                                                       atGTTTTTAAATTCTTACTCAGCCCGCTCACCCCGCCGGCGCAAATCTTTTTCGCAACCAAAAAAAAATTAACGGCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGttt                            <  8:243720/138‑1 (MQ=255)
                                                                                                              tAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTAtctgtc                    >  5:231872/1‑134 (MQ=255)
                                                                                                                aaaCCGTACTGAACCCCGTCCCCCCGCCGGCGGCAATATTATTCGGAACAAAAAATACATTAAAGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTAc                  <  8:93741/139‑1 (MQ=255)
                                                                                                                   tCGTACTGACCCCGCTCCCCCCCCCGGCGTAAATCTTATTCGTAACAAAAAAAACATAAACGCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTc               <  2:53212/139‑1 (MQ=255)
                                                                                                                           aTCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCatat       >  7:93741/1‑139 (MQ=255)
                                                                                                                                gCTGCCCCCGCCGGCGCCAATCTTATTCGCAAAAAAAAAAACATTACACCCCACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATAtcct  <  1:40501/139‑1 (MQ=255)
                                                                                                                                gCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATAtcct  >  4:40761/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                      
CCTGCAAGCGTCTTTAGTCCAAATTTCCCGCCGAGCAAAATGACGCCAGCAATAAATAAAGGGATATTAATAATCCACTGTACATACGCTGCTTCAAAACCATATGATTGTAAAATCGTACTGATCCCGCTGACCCCGCCGGCGGCAATCTTATTCGGAAGCAAAAATACATTAAAGGACACGGCCGTAATCGCTGCTCCTATCAATATGTATACATAATCCCGCAAAATCCACAATGTTTTATTCCGTACGTCCATCATATATCCT  >  CP006881/3444571‑3444837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑