Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,022	G→A	100%	intergenic (‑136/‑165)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,022	0	G	A	100.0%	320.9 / NA	94	intergenic (‑136/‑165)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/59);  total (35/59)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGTTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATG  >  CP006881/3722887‑3723156                                                                                                                                        |                                                                                                                                         cTGTTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTaag                                                                                                                                        >  7:262412/1‑138 (MQ=255)    tttCTCCCCCTTTTATTTAAACAAAAAAGACCTAAACTTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGaa                                                                                                                                      <  4:32562/137‑1 (MQ=255)      tctcCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACg                                                                                                                                  >  8:166904/1‑139 (MQ=255)         cccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCtt                                                                                                                               >  5:25348/1‑139 (MQ=255)          ccccTTTTATTTAAACAAAAAATACCTAAAATTCGCCAAAGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCttt                                                                                                                              <  2:271595/139‑1 (MQ=255)          ccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCttt                                                                                                                              >  8:25516/1‑139 (MQ=255)           cccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCttt                                                                                                                              >  2:23055/1‑138 (MQ=255)            ccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtca                                                                                                                            >  7:273436/1‑139 (MQ=255)            ccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGATTtca                                                                                                                            >  7:273439/1‑139 (MQ=255)              ttttATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcat                                                                                                                           >  4:71947/1‑138 (MQ=255)                ttatttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcatca                                                                                                                         <  6:25348/138‑1 (MQ=255)                ttatttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcatca                                                                                                                         <  3:280811/138‑1 (MQ=255)                ttatttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcatca                                                                                                                         <  1:189941/138‑1 (MQ=255)                ttatttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcatca                                                                                                                         <  6:144375/138‑1 (MQ=255)                  atttaAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATa                                                                                                                       <  5:31505/138‑1 (MQ=255)                           aaaaaGACCTAAAATTCTCCAACGAATAAAGGACACAGCGATACTTTTTCATTTACGCAAATTTTATGTCTTGCCTGCTTTACCAGTCACAATCACGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATa                                                                                                             <  7:68776/139‑1 (MQ=255)                              aaGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCg                                                                                                          <  8:19947/139‑1 (MQ=255)                                 aCCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATa                                                                                                             <  5:300684/133‑1 (MQ=255)                                 aCCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATa                                                                                                             >  6:300684/1‑133 (MQ=255)                                         ttCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAc                                                                                               <  5:156613/139‑1 (MQ=255)                                           cGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑ta                                                                                            >  3:107909/1‑138 (MQ=255)                                                 cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Atttttt                                                                                     >  5:57516/1‑139 (MQ=255)                                                 cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Atttttt                                                                                     <  6:197748/139‑1 (MQ=255)                                                 cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Atttttt                                                                                     >  3:58654/1‑139 (MQ=255)                                                 cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Atttttt                                                                                     <  3:61683/139‑1 (MQ=255)                                                         ggACAAAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTaaa                                                                              <  1:82646/139‑1 (MQ=255)                                                          gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTaaa                                                                              >  3:257806/1‑138 (MQ=255)                                                          gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTaaa                                                                              >  5:116519/1‑138 (MQ=255)                                                            cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAg                                                                           >  1:34775/1‑139 (MQ=255)                                                             acaGCGATGCTTTTTCATTTACGCAAATTTTATGTCTTGCCTTCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                          <  4:256151/139‑1 (MQ=255)                                                             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTTAATatt                                                                          >  1:156234/1‑137 (MQ=255)                                                             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                          <  1:1446/139‑1 (MQ=255)                                                             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                          >  5:177963/1‑139 (MQ=255)                                                              caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGtt                                                                         <  1:74888/139‑1 (MQ=255)                                                              caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGtt                                                                         >  6:75281/1‑139 (MQ=255)                                                               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGttt                                                                        >  2:252921/1‑139 (MQ=255)                                                               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGttt                                                                        >  7:173412/1‑139 (MQ=255)                                                               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                          >  3:41979/1‑137 (MQ=255)                                                               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                          <  4:41979/137‑1 (MQ=255)                                                               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCACGCTTATTCAGATTAAGAATACGCTTTAATCATAAATCATGATAGCGTTTTCGTCTAC‑TATTTTTTTAGTTAAATAGttt                                                                        >  3:98801/1‑139 (MQ=255)                                                                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATat                                                                   <  7:25516/139‑2 (MQ=255)                                                                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATa                                                                    >  7:106063/1‑138 (MQ=255)                                                                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATa                                                                    >  5:306911/1‑138 (MQ=255)                                                                    tCCTTTTTCATTTAAGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATat                                                                   <  1:252456/139‑2 (MQ=255)                                                                           tCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAAcatg                                                             >  8:282363/1‑135 (MQ=255)                                                                            aaTTTACGCAAAATTTAGGTCTTGCCTGCTTTACCAGGCACAATCCCTCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACAA‑TTTTTTTAGTTAAATAGTTTGATATAAcatgt                                                            <  6:294885/137‑5 (MQ=255)                                                                                                   gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                       <  4:74826/136‑3 (MQ=255)                                                                                                         tttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGa                               >  2:51070/1‑138 (MQ=255)                                                                                                         tttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGa                               >  6:80953/1‑138 (MQ=255)                                                                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  1:252921/138‑3 (MQ=255)                                                                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                          >  4:74892/1‑139 (MQ=255)                                                                                                              cAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                         <  5:80953/139‑1 (MQ=255)                                                                                                              cAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                         <  1:51070/139‑1 (MQ=255)                                                                                                                   acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                    <  4:76140/139‑1 (MQ=255)                                                                                                                     aaTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACaa                   >  8:271986/1‑138 (MQ=255)                                                                                                                      atgccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTATTTGTGAAATACTTCACAata                 <  4:116602/136‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACTCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  6:183413/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGGTATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  3:293262/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCCCAata                 <  1:160801/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  6:210851/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:173412/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:258053/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:106063/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  1:23055/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:261022/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  7:282363/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:262412/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  4:58654/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  7:271986/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  4:257806/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  7:175664/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  3:279267/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  7:170958/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  7:166904/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  2:34775/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  6:57516/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  6:306911/139‑1 (MQ=255)                                                                                                                        cccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGGTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc               <  8:273436/139‑1 (MQ=255)                                                                                                                         cagCTTATTCAGATTAATAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc               <  8:273439/136‑1 (MQ=255)                                                                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            >  7:292320/1‑139 (MQ=255)                                                                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  5:75281/139‑1 (MQ=255)                                                                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  4:107909/139‑1 (MQ=255)                                                                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  1:88685/139‑1 (MQ=255)                                                                                                                            cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc           >  7:72640/1‑139 (MQ=255)                                                                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt          <  8:292320/137‑1 (MQ=255)                                                                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt          <  6:116519/137‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                             >  8:244819/1‑112 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                             <  7:244819/112‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 >  1:209909/1‑124 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 >  1:248750/1‑124 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  2:248750/124‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  2:209909/124‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            >  4:290689/1‑129 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  3:290689/129‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg  >  7:63083/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                         CTGTTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATG  >  CP006881/3722887‑3723156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑