Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,067	G→T	100%	intergenic (‑181/‑120)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,067	0	G	T	96.2%	315.4 / 0.5	94	intergenic (‑181/‑120)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (36/54);  minor base A (2/2);  total (38/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAA  >  CP006881/3722935‑3723192                                                                                                                                       |                                                                                                                              cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Atttttt                                                                                                                         <  3:61683/139‑1 (MQ=255) cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Atttttt                                                                                                                         >  3:58654/1‑139 (MQ=255) cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Atttttt                                                                                                                         >  5:57516/1‑139 (MQ=255) cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑T‑Atttttt                                                                                                                         <  6:197748/139‑1 (MQ=255)         ggACAAAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTaaa                                                                                                                  <  1:82646/139‑1 (MQ=255)          gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTaaa                                                                                                                  >  3:257806/1‑138 (MQ=255)          gACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTaaa                                                                                                                  >  5:116519/1‑138 (MQ=255)            cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAg                                                                                                               >  1:34775/1‑139 (MQ=255)             acaGCGATGCTTTTTCATTTACGCAAATTTTATGTCTTGCCTTCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                                                              <  4:256151/139‑1 (MQ=255)             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTTAATatt                                                                                                              >  1:156234/1‑137 (MQ=255)             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                                                              <  1:1446/139‑1 (MQ=255)             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                                                              >  5:177963/1‑139 (MQ=255)              caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGtt                                                                                                             >  6:75281/1‑139 (MQ=255)              caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGtt                                                                                                             <  1:74888/139‑1 (MQ=255)               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGttt                                                                                                            >  2:252921/1‑139 (MQ=255)               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGttt                                                                                                            >  7:173412/1‑139 (MQ=255)               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                                                              >  3:41979/1‑137 (MQ=255)               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGt                                                                                                              <  4:41979/137‑1 (MQ=255)               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCACGCTTATTCAGATTAAGAATACGCTTTAATCATAAATCATGATAGCGTTTTCGTCTAC‑TATTTTTTTAGTTAAATAGttt                                                                                                            >  3:98801/1‑139 (MQ=255)                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATat                                                                                                       <  7:25516/139‑2 (MQ=255)                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATa                                                                                                        >  5:306911/1‑138 (MQ=255)                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATa                                                                                                        >  7:106063/1‑138 (MQ=255)                    tCCTTTTTCATTTAAGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATat                                                                                                       <  1:252456/139‑2 (MQ=255)                           tCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAAcatg                                                                                                 >  8:282363/1‑135 (MQ=255)                            aaTTTACGCAAAATTTAGGTCTTGCCTGCTTTACCAGGCACAATCCCTCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACAA‑TTTTTTTAGTTAAATAGTTTGATATAAcatgt                                                                                                <  6:294885/137‑5 (MQ=255)                                                   gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                                                           <  4:74826/136‑3 (MQ=255)                                                         tttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGa                                                                   >  2:51070/1‑138 (MQ=255)                                                         tttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGa                                                                   >  6:80953/1‑138 (MQ=255)                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                               <  1:252921/138‑3 (MQ=255)                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                                                              >  4:74892/1‑139 (MQ=255)                                                              cAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                                             <  1:51070/139‑1 (MQ=255)                                                              cAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACt                                                             <  5:80953/139‑1 (MQ=255)                                                                   acaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                        <  4:76140/139‑1 (MQ=255)                                                                     aaTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACaa                                                       >  8:271986/1‑138 (MQ=255)                                                                      atgccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTATTTGTGAAATACTTCACAata                                                     <  4:116602/136‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACTCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  6:183413/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGGTATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  3:293262/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCCCAata                                                     <  1:160801/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  6:57516/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  6:306911/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  1:23055/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  6:210851/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  7:166904/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  7:170958/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  7:175664/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  7:271986/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  7:282363/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  8:106063/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  2:34775/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  8:173412/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  3:279267/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  8:258053/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  4:58654/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  8:261022/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  8:262412/139‑1 (MQ=255)                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  4:257806/139‑1 (MQ=255)                                                                        cccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGGTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                   <  8:273436/139‑1 (MQ=255)                                                                         cagCTTATTCAGATTAATAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                   <  8:273439/136‑1 (MQ=255)                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                >  7:292320/1‑139 (MQ=255)                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                <  5:75281/139‑1 (MQ=255)                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                <  1:88685/139‑1 (MQ=255)                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                <  4:107909/139‑1 (MQ=255)                                                                            cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                               >  7:72640/1‑139 (MQ=255)                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                              <  8:292320/137‑1 (MQ=255)                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                              <  6:116519/137‑1 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                 >  8:244819/1‑112 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                 <  7:244819/112‑1 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     >  1:248750/1‑124 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     >  1:209909/1‑124 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  2:209909/124‑1 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  2:248750/124‑1 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                >  4:290689/1‑129 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                <  3:290689/129‑1 (MQ=255)                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                      >  7:63083/1‑139 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                               <  2:190980/104‑3 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                               >  1:190980/1‑102 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                          >  7:171675/1‑109 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                          <  8:171675/109‑1 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                   >  5:228941/1‑116 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                   <  6:228941/116‑1 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                             >  2:260905/1‑138 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                             >  7:161387/1‑138 (MQ=255)                                                                                               cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                             >  4:58527/1‑138 (MQ=255)                                                                                                     catcatAAATCATGATAGCGTTTTCGTCAACAA‑TTTTTTTAGTTAAATAGTTTGATATAACATGTAGAAAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                       >  2:41505/1‑138 (MQ=255)                                                                                                       tcatAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                    <  3:74892/139‑1 (MQ=255)                                                                                                          tAAATCATGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAg                 >  8:155898/1‑139 (MQ=255)                                                                                                                aTGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaa              >  2:32821/1‑136 (MQ=255)                                                                                                                aTGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaa              >  8:250644/1‑136 (MQ=255)                                                                                                                 tGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     >  4:31739/1‑96 (MQ=255)                                                                                                                 tGATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                     <  3:31739/96‑1 (MQ=255)                                                                                                                  gATAGCGTTTTCGTCAAC‑TATTTTTTTAGTTAAATTGTTTGATTTAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGATATACTATGCTCAATCATGAAAGAAAGAAGGaaaaga          >  6:174143/1‑136 (MQ=255)                                                                                                                       cGTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATgg    <  3:58527/139‑1 (MQ=255)                                                                                                                        gTTTTCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa   <  4:169964/139‑1 (MQ=255)                                                                                                                         ttttCGTCAAC‑TATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGaa  <  1:32821/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                              CGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑T‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAA  >  CP006881/3722935‑3723192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑