| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,578,472 | (A)7→6 | intergenic (‑65/‑70) | U712_18365 ← / → U712_18370 | Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,578,466 | 0 | A | . | 94.5% | 220.2 / 6.7 | 55 | intergenic (‑59/‑76) | U712_18365/U712_18370 | Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base . (21/31); total (22/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGATT > CP006881/3578329‑3578598 | ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa > 7:244422/1‑138 (MQ=255)ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCACCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa > 5:114094/1‑138 (MQ=255)ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTATTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa > 2:312644/1‑138 (MQ=255) tGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATAACCTCAGCAACATTCGGAACACTTTTCTGAAAATATATACAACCTATCCaa > 5:241415/1‑138 (MQ=255) aGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGGTTCATGACCTCAGCAACATTCTGCACACTTTTCTGAAAATATATACAACCTATCCaaaaaa > 6:76960/1‑139 (MQ=255) ggAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTATTTTATTGCTCTCTCATTGTTTCATCACCTCAGCAACAATCTGAACACTTTTGTGAAAATTTATACAACCTATCCaaaaaact > 6:312500/1‑137 (MQ=255) cacCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACttttt < 4:201051/139‑1 (MQ=255) gCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGAt > 6:61128/1‑139 (MQ=255) gCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATg > 1:277509/1‑139 (MQ=255) gCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATAAAACCTATCC‑AAAAAACTTTTTTTGATg > 3:213750/1‑139 (MQ=255) tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGt < 4:194613/138‑1 (MQ=255) tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATAACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGtt > 6:116733/1‑139 (MQ=255) aaCGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGt < 2:337873/137‑1 (MQ=255) aCGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTATCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTCAAAATATATACAACCTATCC‑AAACAACTTTATTAGATTTAGttt > 7:333055/1‑139 (MQ=255) aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca < 8:262032/139‑1 (MQ=255) aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca < 8:244422/139‑1 (MQ=255) aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca < 3:198959/139‑1 (MQ=255) aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca < 1:157045/139‑1 (MQ=255) aaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca < 2:277509/138‑1 (MQ=255) aaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGa < 6:149523/139‑1 (MQ=255) tGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTAtttt > 7:229495/1‑139 (MQ=255) cttttcatCCTCTCTCCTTGTTTCATCACCTCAGCAACAATCAGAACCCGATTCTGAAAATATATACAACCTAT‑CAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAgagga < 8:256564/138‑1 (MQ=255) ttcttcCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAgaggag < 8:229495/136‑1 (MQ=255) ttcttcCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAgaggag < 5:312500/136‑1 (MQ=255) ttcttcCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAgaggag < 1:312644/136‑1 (MQ=255) ttcCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCa > 5:296793/1‑138 (MQ=255) ccTCTCTCATTGATTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCaaa < 8:189339/138‑1 (MQ=255) ctctcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTTAAAATATAAACAACCTATCC‑AAAAAACTTTTTTATATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACg > 1:16456/1‑139 (MQ=255) tcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAATATATATACTACCTATCC‑AATAAACTTTTTTAGATGTAGTATGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGt < 3:112497/137‑1 (MQ=255) tcATTGTTTCATCACCTCAGCAACATTCTGAACACATTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGt < 2:223864/137‑1 (MQ=255) aTTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTAt < 7:224746/137‑1 (MQ=255) tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa < 1:36068/138‑1 (MQ=255) tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa < 6:114094/138‑1 (MQ=255) tcatcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGa > 8:188431/1‑139 (MQ=255) atcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCaaa > 3:152395/1‑122 (MQ=255) atcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCaaa < 4:152395/122‑1 (MQ=255) gCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCtt > 7:185681/1‑139 (MQ=255) ctaCATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTg < 6:296793/137‑1 (MQ=255) aCATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt < 5:116733/139‑1 (MQ=255) aCATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt < 7:188431/139‑1 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCC‑AAAACGTATGAACATGACATTGCGTCTTGCTGAACATa < 5:61128/138‑1 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGGGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACa > 1:95605/1‑136 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt > 7:61524/1‑131 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt > 1:304919/1‑131 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt < 8:61524/131‑1 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt < 2:304919/131‑1 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACa > 2:226454/1‑136 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACa > 2:132057/1‑136 (MQ=255) aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa < 2:26509/138‑1 (MQ=255) acaaTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGGGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATaga < 2:95605/139‑1 (MQ=255) ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACt > 6:154763/1‑139 (MQ=255) ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACt > 5:95100/1‑139 (MQ=255) ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACt > 5:160523/1‑139 (MQ=255) tCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTg < 6:160523/138‑1 (MQ=255) aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAgg < 8:185681/138‑1 (MQ=255) aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAgg < 5:76960/138‑1 (MQ=255) aCAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTgg < 2:16456/138‑1 (MQ=255) ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTg > 7:214663/1‑104 (MQ=255) ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTg < 8:214663/104‑1 (MQ=255) ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGAtt > 4:304269/1‑139 (MQ=255) ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGAtt > 5:276109/1‑139 (MQ=255) | TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGATT > CP006881/3578329‑3578598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |