| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 1,364,839 | (A)8→7 | coding (30/1695 nt) | U712_06990 → | putative membrane protein ykoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 1,364,832 | 0 | A | . | 96.7% | 253.8 / 0.8 | 60 | coding (23/1695 nt) | U712_06990 | putative membrane protein ykoS |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base . (27/31); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGATTGTCATATGATGAATGATTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACAAAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCTTGGTACAAGG > CP006881/1364702‑1364966 | aCGGATTGTCATATGATGAATGATTTGGAAGGGAAAATGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACaaaaaaaga < 1:264827/139‑3 (MQ=255)aCGGATTGTCATATGATGAATGATTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACaaaaaaaga < 4:78055/139‑3 (MQ=255)aCGGATTGTCATATGATGAATGATTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACaaaaaaaga < 1:265349/139‑3 (MQ=255) ggATTGTCATATGATGAATGATTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACaaaaaaagaaa < 8:248374/139‑5 (MQ=255) ggATTGTCATATGATGAATGATTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACaaaaaaagaaa < 7:214475/139‑5 (MQ=255) ggATTGTCATATGATGAATGATTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACaaaaaaagaaa < 6:31878/139‑5 (MQ=255) gATTGTCATATGATGTATGATTTGGACGGGAATAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAAc < 5:182121/139‑1 (MQ=255) tCATATGATGAATGATTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACatat < 2:225191/138‑1 (MQ=255) aattaTTTGGAAGGGAAAAGGGATAATAAATTTTTACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTAACGATTGATGAGACAA‑AAAAAAAAGAAACATATTATCTCTGGa > 5:180841/1‑138 (MQ=255) aatgaTTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACat < 6:155479/126‑1 (MQ=255) aatgaTTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACat > 5:155479/1‑126 (MQ=255) aatgaTTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGAt > 1:58097/1‑139 (MQ=255) aatgaTTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGAt > 4:244183/1‑139 (MQ=255) aatgaTTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGAt < 7:116304/139‑1 (MQ=255) aatgaTTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGAt > 2:335459/1‑139 (MQ=255) aatgaTTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGAt > 5:251465/1‑139 (MQ=255) tttGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACAAGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGTATCGCat > 4:29621/1‑139 (MQ=255) tGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCatta > 5:160623/1‑139 (MQ=255) aGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATttc < 1:335459/139‑1 (MQ=255) aGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATttc < 6:160623/139‑1 (MQ=255) ggAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATttctt < 4:131071/139‑1 (MQ=255) gggATAATAAATTTTGAAACATGAGGCAGAACATGCCGACGGAGTCAGAATTACATATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAAAATATTATCTCTGGATCGCATTATTTCTTCTTTTa < 3:259483/139‑1 (MQ=255) gATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTAtt < 2:101123/139‑1 (MQ=255) ataaATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTcttctt < 6:37213/130‑1 (MQ=255) ataaATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTcttctt > 5:37213/1‑130 (MQ=255) aTGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCtttt < 1:232915/139‑1 (MQ=255) tccGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGATACAACAAAAAAAA‑AAACATATTATATCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCAGCTTTTTATTTTaaatg > 4:291984/2‑134 (MQ=255) gCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACaaaaaaaga < 1:162497/68‑3 (MQ=255) gCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACaaaaaaaga > 2:162497/1‑66 (MQ=255) gCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAgg > 4:117819/1‑136 (MQ=255) gCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAgg > 4:133243/1‑136 (MQ=255) gCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAgg > 6:174767/1‑136 (MQ=255) gCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGGTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTAATTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAg < 6:17176/139‑1 (MQ=255) ccGACGGAGTCAGAATAACAAATGGATGGGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGa < 8:194884/139‑1 (MQ=255) cGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAATGAAGATGcc > 1:175254/1‑139 (MQ=255) cGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGcc > 2:272713/1‑139 (MQ=255) cGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTGTTATTTTAAAGGAAGATGcc > 3:286568/1‑139 (MQ=255) ggAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGccc < 2:58097/139‑1 (MQ=255) cAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTa < 1:120389/94‑1 (MQ=255) cAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTa > 2:120389/1‑94 (MQ=255) aTAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGTAAGATGCCCATATCCgg < 4:171406/138‑1 (MQ=255) cAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAg > 2:110564/1‑120 (MQ=255) cAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAg < 1:110564/120‑1 (MQ=255) cAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCa < 3:244183/139‑1 (MQ=255) cAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCa > 6:299088/1‑139 (MQ=255) aaTGGATGTGTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTATATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACg < 8:105749/139‑1 (MQ=255) aaTGGATGTGTAAGCAATGTTAAGATTGATGACACAAC‑TAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAACGAAGATGCCCATATCCGGGTCCAct < 8:184557/139‑2 (MQ=255) tgtgTAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAAtt < 3:117819/139‑1 (MQ=255) tgtAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCGCATATCCGGGTCCACGATAAta > 2:183708/1‑136 (MQ=255) tgtAAGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTa > 1:189790/1‑139 (MQ=255) aGCAATGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGAt > 3:42647/1‑138 (MQ=255) aaTGTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCACGCTTTTTATTTTAAAGGAAGATGCACATATCCTGGTCCACGATAATTTAGATTcc > 1:44676/1‑138 (MQ=255) gTTACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATGCGGGTCCACGATAATTTAGATTCCAACa > 3:142909/1‑139 (MQ=255) ttACGATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGGCCACGATAATTTAGATTCCAACa < 1:183708/138‑1 (MQ=255) gATTGATGAGAGAAC‑ATATAAAGAAACTTATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCAGCTTTTTATTTTAAAGGAAGATTCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCt < 5:84715/139‑1 (MQ=255) gATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCt < 1:156966/139‑1 (MQ=255) gATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCt < 1:272713/139‑1 (MQ=255) gATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCt < 6:251465/139‑1 (MQ=255) gATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCt < 6:140524/139‑1 (MQ=255) gATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCt < 3:83691/139‑1 (MQ=255) gATTGATGAGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGATTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCt < 3:316806/139‑1 (MQ=255) gATTGATGACACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTAACCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCt < 4:38952/139‑1 (MQ=255) gatgaGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAATGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCTTgg > 3:150478/1‑138 (MQ=255) gatgaGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCCCGATAAGTTATATTCCAACATAGCTTgg > 6:183814/1‑138 (MQ=255) gatgaGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCTTGGt > 5:46170/1‑139 (MQ=255) atgaGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCTTGGTa > 5:169970/1‑139 (MQ=255) tgaGACAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTTTTTTAAAAGAAGATTCCCAAATCCGGGTCCACCATAATTTTAATTACAACAACGCTTTGtt > 2:156966/1‑137 (MQ=255) agaCAAC‑AAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCTTGGTACa < 5:174767/138‑1 (MQ=255) acaac‑aAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCTTGGTACAAgg > 8:81654/1‑139 (MQ=255) | ACGGATTGTCATATGATGAATGATTTGGAAGGGAAAAGGGATAATAAATTTTGACACATGAGGCAGAACATGCCGACGGAGTCAGAATAACAAATGGATGTGTAAGCAATGTTACGATTGATGAGACAACAAAAAAAAGAAACATATTATCTCTGGATCGCATTATTTCTTCTTTTATTGTATGTATCCCCGCTTTTTATTTTAAAGGAAGATGCCCATATCCGGGTCCACGATAATTTAGATTCCAACATCGCTTGGTACAAGG > CP006881/1364702‑1364966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |