Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,822 A→G S212S (TCA→TCG)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,8220AG95.1% 253.8 / ‑1.4 82S212S (TCA→TCG) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (34/44);  minor base T (2/2);  total (36/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCG  >  CP006881/3723688‑3723949
                                                                                                                                      |                                                                                                                               
tgCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCttt                                                                                                                             >  3:316851/1‑139 (MQ=255)
  ccTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCtttt                                                                                                                            >  2:65061/1‑138 (MQ=255)
   cTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTGTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGa                                                                                                                          >  1:271039/1‑139 (MQ=255)
   cTCGCCTTTTTCAATCCAATCTCTCTCAAAGGGGAAGGATCAAAGCTTTATACCATGATGCTTTTCGTATTCTGCTTCTTTATTTACGTCTTTGAAAACAGCATTGCCAATATGTTCACATTCGCAATCTCTCtttag                                                                                                                           >  6:13903/1‑136 (MQ=255)
        cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  1:65061/139‑1 (MQ=255)
        cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  6:292845/139‑1 (MQ=255)
        cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  4:316851/139‑1 (MQ=255)
        cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  5:266543/139‑1 (MQ=255)
        cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  5:306924/139‑1 (MQ=255)
        cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  1:122959/139‑1 (MQ=255)
        cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGAAAAGCTTTTTACAATGATGCTTTTCGATTTCTGCTTCTTTATTTCCGGATTTGAACACAGCATTGCAAATATGTGCACATTCGACATCTCGCTTTTGATCGAg                                                                                                                     >  6:126392/1‑139 (MQ=255)
        cTTTATCATTCCAATGTCTTTCCACGGGGAATGAGAAAAGCTTTTTACCATTATGCTTTTCTTTGTCTGCTTCTTTCTTTCCGGCGTTGAACACAGCATTGCCAATATGTGCCCATTCGCCATCTAGCTTTTGATctat                                                                                                                     <  5:227300/139‑4 (MQ=255)
        atttttCATTCCAATGTCTCTCAAAGGGTAAGGAGAAAAGCTTTTTAGCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCTCTTTTGATCGAg                                                                                                                     <  5:13903/138‑1 (MQ=255)
          ttttCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATTTGCACATTCGCCATCTCGCTTTTGATCGAGCa                                                                                                                   >  4:134663/1‑139 (MQ=255)
           tttCATTCCATTGTCTCTCAAAGGTGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCa                                                                                                                   <  5:323666/138‑1 (MQ=255)
                ttCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTg                                                                                                              >  4:167162/1‑138 (MQ=255)
                ttCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGa                                                                                                             <  3:134663/139‑1 (MQ=255)
                ttCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGATTTTTATCGAGCACCCTGa                                                                                                             <  5:305060/139‑1 (MQ=255)
                            tcaaaGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACa                                                                                                   <  6:230282/135‑1 (MQ=255)
                            tcaaaGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACa                                                                                                   <  3:144088/135‑1 (MQ=255)
                                  gggaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATgg                                                                                            >  6:92759/1‑138 (MQ=255)
                                    gaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAg                                                                                         <  3:167162/139‑1 (MQ=255)
                                           cAAAGCTTTTTACGATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAg                                                                                  <  3:23714/139‑1 (MQ=255)
                                              aGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGaa                                                                                >  7:50357/1‑138 (MQ=255)
                                                   tttACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCTCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCa                                                                                   <  5:233874/130‑1 (MQ=255)
                                                   tttACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCTCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCa                                                                                   >  6:233874/1‑130 (MQ=255)
                                                   tttACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     >  1:243665/1‑96 (MQ=255)
                                                   tttACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACtt                                                                            <  5:92759/137‑1 (MQ=255)
                                                   tttACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTCCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  2:243665/96‑1 (MQ=255)
                                                     tACCATGATTCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGAGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAAt                                                                         <  5:126392/138‑1 (MQ=255)
                                                        cATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATcccc                                                                     >  6:308822/1‑139 (MQ=255)
                                                        cATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATcccc                                                                     >  6:303981/1‑139 (MQ=255)
                                                          tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  5:223271/89‑1 (MQ=255)
                                                          tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     >  6:223271/1‑89 (MQ=255)
                                                          tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGCCACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                   >  3:234700/1‑139 (MQ=255)
                                                          tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                   >  5:105679/1‑139 (MQ=255)
                                                          tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                   >  1:95616/1‑139 (MQ=255)
                                                          tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                   >  7:124019/1‑139 (MQ=255)
                                                          tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                                                   >  4:260956/1‑139 (MQ=255)
                                                                         tCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCt                                                    >  2:226099/1‑139 (MQ=255)
                                                                                ctttATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCt                                                                                                               >  1:338238/1‑73 (MQ=255)
                                                                                ctttATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCt                                                                                                               <  2:338238/73‑1 (MQ=255)
                                                                                ctttATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                              <  2:119110/138‑1 (MQ=255)
                                                                                ctttATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACcgc                                              <  2:271039/138‑1 (MQ=255)
                                                                                     tttCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                         <  6:105679/138‑1 (MQ=255)
                                                                                     tttCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                         <  3:7831/138‑1 (MQ=255)
                                                                                     tttCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                         <  8:124019/138‑1 (MQ=255)
                                                                                           gCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTAACGCTCGGCAATCTGACCGCGGGAATAGTTat                                  >  6:84684/1‑139 (MQ=255)
                                                                                            cgttgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatg                                 <  4:310179/135‑1 (MQ=255)
                                                                                               tgAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTatga                                <  3:309067/136‑1 (MQ=255)
                                                                                                 aaCACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGATACGCTCGGCAATCTGACCGCGGGAATAGTTATGATggg                            <  8:50357/139‑1 (MQ=255)
                                                                                                  acacAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGc                           <  4:234700/139‑1 (MQ=255)
                                                                                                  acacAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGc                           <  2:95616/139‑1 (MQ=255)
                                                                                                   cacaGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCt                          <  1:226099/139‑1 (MQ=255)
                                                                                                   cacaGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCt                          <  2:314985/139‑1 (MQ=255)
                                                                                                     caGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTgg                        <  1:332760/139‑1 (MQ=255)
                                                                                                      aGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     >  5:16709/1‑45 (MQ=255)
                                                                                                      aGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  6:16709/45‑1 (MQ=255)
                                                                                                      aGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACACTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGa                       >  5:174552/1‑139 (MQ=255)
                                                                                                      aGCATTGCCAATATGTGCAAATTATCCATCTCGATTTTGATCGAGCAACCTTATACAGTTACACTGATGGGAGAAGTCAGAAACTTAATCACAGTTACGCTCTGCAATCTGCACGCGGGAATAGTTATGATGGTCTGGa                       >  2:223709/1‑139 (MQ=255)
                                                                                                       gCATTGCCAATATGTTCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                      <  5:303981/139‑1 (MQ=255)
                                                                                                       gCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                      <  5:308822/139‑1 (MQ=255)
                                                                                                              cAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac               <  6:120760/139‑1 (MQ=255)
                                                                                                                atatGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     <  8:305278/35‑1 (MQ=255)
                                                                                                                atatGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                                                     >  7:305278/1‑35 (MQ=255)
                                                                                                                atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCTGAAACTTAATCCCCGTTAAGCTCGGCAATCTGACCGCGGGa                                          <  3:126637/110‑1 (MQ=255)
                                                                                                                atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGa                                          >  4:126637/1‑110 (MQ=255)
                                                                                                                atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTaca              >  8:134594/1‑138 (MQ=255)
                                                                                                                  atGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAg                                                                                                        <  1:274172/46‑1 (MQ=255)
                                                                                                                  atGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAg                                                                                                        >  2:274172/1‑46 (MQ=255)
                                                                                                                  atGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTacacac           >  1:57128/1‑137 (MQ=255)
                                                                                                                     tgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                         >  5:104785/1‑106 (MQ=255)
                                                                                                                     tgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa                                         <  6:104785/106‑1 (MQ=255)
                                                                                                                       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAAtag                                      <  3:34530/107‑3 (MQ=255)
                                                                                                                       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAAtag                                      >  4:34530/1‑105 (MQ=255)
                                                                                                                       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                      <  6:295624/123‑1 (MQ=255)
                                                                                                                       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                      <  2:5928/123‑1 (MQ=255)
                                                                                                                       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                      >  1:5928/1‑123 (MQ=255)
                                                                                                                       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGAt                      >  5:295624/1‑123 (MQ=255)
                                                                                                                       cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt      >  2:210991/1‑139 (MQ=255)
                                                                                                                       cacaTTCGCCATCTCGCTTTAGATCGAGCTCCCTGATACAGTCACACTGATGGGAGCAGTCAGAAACTTAATCCCCGATACGCTCGGCAAGCTGACCTCGCGAATAGTGATGATGGGCTGGATGTACTACACACTGAAt      >  7:146813/1‑139 (MQ=255)
                                                                                                                           ttCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGAGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATcctg  <  6:174552/139‑3 (MQ=255)
                                                                                                                                      |                                                                                                                               
TGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCG  >  CP006881/3723688‑3723949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑