Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 1,969,141 T→C I259V (ATC→GTC)  U712_09725 ← Pyrroline‑5‑carboxylate reductase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,969,1410TC97.7% 142.1 / NA 43I259V (ATC→GTC) U712_09725Pyrroline‑5‑carboxylate reductase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (20/22);  minor base A (0/1);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGAATGCTGATTTCTCCATGAAGGCTTGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCTT  >  CP006881/1969016‑1969274
                                                                                                                             |                                                                                                                                     
ttCGAATGCTGATTTCTCCATGAAGGCTTGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGAt                                                                                                                           <  8:114955/138‑1 (MQ=255)
        ctcagttCTCCATGAAGGCTTGTAAATGACCTGCTGCCAATCTGCACACCGACTCTTTTCATGCGTGAATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTg                                                                                                                   <  3:193227/133‑1 (MQ=255)
        cTGATTTCTCCATGAAGGCTTGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTg                                                                                                                   <  7:280188/138‑1 (MQ=255)
              tctcCATGAAGGCTTGTTAATGACCTCCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCt                                                                                                             <  7:218621/138‑1 (MQ=255)
                  cATGAAGGCTTGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGcc                                                                                                        >  5:70256/1‑139 (MQ=255)
                    tGAAGGCTTGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGc                                                                                                      <  7:79401/139‑1 (MQ=255)
                    tGAAGGCTTGTAAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTCTCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGc                                                                                                      <  2:70368/139‑1 (MQ=255)
                     gAAGGCTTGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCa                                                                                                     <  2:225474/139‑1 (MQ=255)
                      aaGGCTTGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCa                                                                                                     <  6:314615/138‑1 (MQ=255)
                                        gCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGaaa                                                                                  >  7:212360/1‑139 (MQ=255)
                                        gCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATATTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGaaa                                                                                  >  8:219831/1‑139 (MQ=255)
                                         cTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCCCTCCTGATAGCGGCGCTGCAGTTTTTTGGCCATCTTGGCTGCGTTCCTTTGTCCGGATAGCCGCCTGCTGTTTTGGTTGAGaaag                                                                                 >  5:266957/1‑138 (MQ=255)
                                         cTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGGTGATTTCCTTTGAACGCTTAGCCGCCTGCGTTATTGCTTGAGaaa                                                                                  >  2:8632/1‑138 (MQ=255)
                                         cTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAAt                                                                                 >  1:222416/1‑139 (MQ=255)
                                                       aaCGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTccgccgc                                                                   <  2:222416/139‑1 (MQ=255)
                                                       aaCGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTccgccgc                                                                   <  4:101368/139‑1 (MQ=255)
                                                                   cATGCTTGTCTCCGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTGCGACATCTTCGCTGATTTCCTTTCAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTc                                                        <  2:187359/138‑1 (MQ=255)
                                                                   cATGCTTGTAGCCGGCGTCACTTTATCACTCCTGATAGCGGCGCGGCAGTTTTTTCGACATCTTCGCGGATTTCCTTTCAACGCGTCGCCGCATGCTTTATTGCTTGCGAAATCGCTTCTCCGCCGCCGCTCTTTTTc                                                        <  8:41246/138‑1 (MQ=255)
                                                                    aTGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCa                                                       <  1:15114/138‑1 (MQ=255)
                                                                       cTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGc                                                   >  5:234150/1‑139 (MQ=255)
                                                                       cTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTACTTTGAACGCTTAGCCGCATGCTTTATTGCTTTAGAAATAGCTTCTCAGCCGCCGCGCGTTTTAAAAGc                                                   >  1:271623/1‑139 (MQ=255)
                                                                         tGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAAAGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCtt                                                 >  6:158264/1‑139 (MQ=255)
                                                                         tGTATCAAGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCGCTTTTTCAAGGCtt                                                 >  5:150599/1‑139 (MQ=255)
                                                                           tATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCTCTTCTCCTCCGCCGCTCTTTTTCAAGGCTTg                                                <  7:51025/138‑1 (MQ=255)
                                                                           tATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGGTTTTTCGACATCTTAGCGGATTTCCGTTGAACCCTTAGCCGCATGCTGTTTTTCTTGATAAATGCCTTCTCCGCCGCCCCACTTTTTCAACGCATgg                                               >  4:219677/1‑138 (MQ=255)
                                                                                ggCGTCACTTTATCACTCCTGATAGCGGCGCTGGAGTTTTTTAGACAACTTCGCTTATTTGCTTTTAACGCTTAGCCGCATGCTTTATTTCTTTAGAAATATCTTCACCGCCCCCCCTCTATTAAAAGGCTTGCAAccc                                          >  8:51025/1‑139 (MQ=255)
                                                                                ggCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGccc                                          <  7:82138/139‑1 (MQ=255)
                                                                                ggCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGccc                                          <  5:158264/139‑1 (MQ=255)
                                                                                ggCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGccc                                          <  1:62605/139‑1 (MQ=255)
                                                                                   gTCACTATATCACACCGGATAGCGGCGCGGCAGTTTTTTCGACATCTGCGCTCATTGCCTTTGAACGCTTAGCCGAATGCTTTATTGCTTTAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCgc                                       <  6:72089/139‑1 (MQ=255)
                                                                                    tCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCGCTTTTTAAAGGCTTGCAGCCCCgcg                                      >  5:76018/1‑138 (MQ=255)
                                                                                     cACTTTATCACTCCTGATAGCGGCGCTGCACTTTTTTGGTCATCTTCGCGGATTTCCGTTGTACGCTTATCCGCCTGCCTTTTTGCTTGAGAAAACGCTGCGCCGCCACCCCTCGTTTTGAAGGGTTGCAGCCCCgcgg                                     >  6:107258/1‑137 (MQ=255)
                                                                                           aTCACTCCTGATAGCGGCGCTTCAGTTTTTTCGCCCTAGTAGCTGATTTACTTTGAACGCGTAGACGCATGCGTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTAAAGGCTTTCCGCCCCgcggcagggg                               >  6:243417/1‑134 (MQ=255)
                                                                                               cTCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTcgttcc                           <  6:150599/139‑1 (MQ=255)
                                                                                                tCCTGATAGCGGCGCTGCAGTTTTTTCGACATCTTAGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCGTTTTCAAGGCTTTCAGCCCCGCGGCGGTcgtgacg                          >  8:70931/1‑135 (MQ=255)
                                                                                                  cTGATAGCGGCGCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTcgttccgtt                        >  5:259036/1‑139 (MQ=255)
                                                                                                          ggcgCTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCTCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACg                <  4:134396/139‑1 (MQ=255)
                                                                                                            cgcTGCAGTTTTTTCGACATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTg              >  3:289588/1‑139 (MQ=255)
                                                                                                            cgcTGCAGTTTTTTCGACATCTTCGCTGAGTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTATCCGCCGCCGCTCTTTTTCAAGGCTTGCCTCCCCGCGGCTGTCGTTCCGTTCGGGGGCGTg              >  7:221616/1‑139 (MQ=255)
                                                                                                            cgcTGCAGTTTTTTCGACATCTTCGATGAATTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTATTTTTCAACGCTAGCCGCCCCCCTGCGTTCGTTACGTTAGGTTAc                 >  6:319594/1‑136 (MQ=255)
                                                                                                                   gTTTTTTCGACATCTTCGCTGATTTCCTTTGACCGCTTAGCCGCATGATTAATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGAtattat       <  5:215336/139‑1 (MQ=255)
                                                                                                                    ttttttCGAAATCTTCGCTGATTTCCTTGTAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGAtattat       <  2:176449/138‑1 (MQ=255)
                                                                                                                         tCGACATCTTCGCTGATTTCCTTTTAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCtt  <  6:70256/138‑1 (MQ=255)
                                                                                                                             |                                                                                                                                     
TTCGAATGCTGATTTCTCCATGAAGGCTTGTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCTT  >  CP006881/1969016‑1969274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑