Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,933	T→C	Y249Y (TAT→TAC)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,933	0	T	C	100.0%	190.9 / NA	51	Y249Y (TAT→TAC)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/19);  total (32/19)

CAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAA  >  CP006881/3723798‑3724062                                                                                                                                        |                                                                                                                                  cAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac                                                                                                                                <  6:120760/139‑1 (MQ=255)   atatGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTaca                                                                                                                               >  8:134594/1‑138 (MQ=255)     atGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTacacac                                                                                                                            >  1:57128/1‑137 (MQ=255)          cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                       >  2:210991/1‑139 (MQ=255)          cacaTTCGCCATCTCGCTTTAGATCGAGCTCCCTGATACAGTCACACTGATGGGAGCAGTCAGAAACTTAATCCCCGATACGCTCGGCAAGCTGACCTCGCGAATAGTGATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                       >  7:146813/1‑139 (MQ=255)              ttCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGAGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATcctg                                                                                                                   <  6:174552/139‑3 (MQ=255)                         gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTacaca                                                                                                                             <  8:332413/117‑2 (MQ=255)                         gcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTacaca                                                                                                                             >  7:332413/2‑117 (MQ=255)                             ttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACt                                                                                                    >  6:46032/1‑139 (MQ=255)                                     gCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCGCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACAAACTGAATCCTGATCAATAAAAAAACTTCCAgaga                                                                                            <  3:260956/139‑1 (MQ=255)                                          cTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACt                                                                                       <  5:84684/139‑1 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac                                                                                                                                >  5:44465/1‑89 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac                                                                                                                                <  6:44465/89‑1 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTa                                                                                                                                 <  8:48126/88‑1 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTa                                                                                                                                 >  7:48126/1‑88 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCag                                                                                               <  4:112476/122‑1 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCag                                                                                               >  3:112476/1‑122 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                              >  4:64065/1‑139 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                              >  1:209776/1‑139 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACGGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                              >  1:252793/1‑139 (MQ=255)                                                   tGACACTGATGGGAGCAGTCAGAAACTTAATCCCAGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                              >  8:27872/1‑139 (MQ=255)                                                     acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGt                                                                             >  8:187926/7‑138 (MQ=255)                                                     acactgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGt                                                                             >  6:244645/7‑138 (MQ=255)                                                       actgaTGGGAGCAGTCAGAAACTTAATCCCCGATACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                          <  5:156148/135‑1 (MQ=255)                                                           aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTcc                                                                                                 >  8:924/1‑112 (MQ=255)                                                           aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTcc                                                                                                 <  7:924/112‑1 (MQ=255)                                                           aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAcgcg                                                                      >  6:191707/1‑139 (MQ=255)                                                           aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg                                                                      >  3:56258/1‑139 (MQ=255)                                                            tGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCgg                                                                     <  1:210991/139‑1 (MQ=255)                                                                     tCAGAAACATAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTCGATGTACTACACACTGAATCCTGATCAATAAAAAAACGTCCAGAGAACACTCCCTGCAAGTCAAAGCGGTTATTcc                                                              >  7:54851/1‑137 (MQ=255)                                                                       aGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATATTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgag                                                           <  7:27872/138‑1 (MQ=255)                                                                         aaaCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCga                                                            >  6:212998/1‑135 (MQ=255)                                                                         aaaCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCga                                                            >  5:229833/1‑135 (MQ=255)                                                                         aaaCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCga                                                            >  5:260015/1‑135 (MQ=255)                                                                               aaTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTg                                                   >  1:42553/1‑138 (MQ=255)                                                                               aaTCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTg                                                   >  8:223357/1‑138 (MQ=255)                                                                                atccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa                                                 <  7:134594/136‑1 (MQ=255)                                                                                     gtttACGCTCCGCAATGTGACCGCGGGATTAGGTATGATGGGCTGGTTGTACTAGACACGGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTGCGAGTGATCTTGAAGACGc                                            <  8:54851/137‑1 (MQ=255)                                                                                      gTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGtactac                                                                                                                                <  4:24196/54‑1 (MQ=255)                                                                                      gTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAACGCGGTTATTCCGAGAGATCTTGAAGACGCt                                           >  8:105304/1‑139 (MQ=255)                                                                                             tCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAACGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                    <  5:244645/139‑1 (MQ=255)                                                                                               ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                          <  2:3078/99‑1 (MQ=255)                                                                                               ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                          >  1:3078/1‑99 (MQ=255)                                                                                               ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                    >  4:309729/1‑137 (MQ=255)                                                                                               ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATg                                    <  5:212998/137‑1 (MQ=255)                                                                                                gCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                 <  4:56258/139‑1 (MQ=255)                                                                                                  aatcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCg                               <  6:229833/136‑1 (MQ=255)                                                                                                              ggAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                          >  4:151003/1‑84 (MQ=255)                                                                                                              ggAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                          <  3:151003/84‑1 (MQ=255)                                                                                                                  tagttatgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTc               >  6:227606/6‑139 (MQ=255)                                                                                                                          atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCg       >  7:294300/1‑139 (MQ=255)                                                                                                                             ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGc     >  5:152347/1‑138 (MQ=255)                                                                                                                               cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGc                      <  6:152376/119‑1 (MQ=255)                                                                                                                               cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGc                      >  5:152376/1‑119 (MQ=255)                                                                                                                               cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTCCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa  >  3:41503/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                  CAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAA  >  CP006881/3723798‑3724062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑