| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,239 | 2 bp→AA | intergenic (‑71/‑457) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,239 | 0 | G | A | 100.0% | 139.1 / NA | 43 | intergenic (‑71/‑458) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/18); total (25/18) | |||||||||||
| * | CP006881 | 3,725,240 | 0 | G | A | 97.6% | 111.6 / NA | 43 | intergenic (‑72/‑457) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (25/16); minor base C (0/1); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑C‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAG > CP006881/3725119‑3725363 || ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAACCATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑C‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAttt > 5:94324/1‑139 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAgttcttt > 7:155857/1‑139 (MQ=255) aTCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCaaa > 7:140267/1‑138 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctg > 5:157389/1‑136 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCGTTTCTCGCAAACTAGCGGctc > 1:276090/1‑137 (MQ=255) tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCGTTTCTCGCAAACTAGCGGctc > 7:274320/1‑137 (MQ=255) tAAAGATACAACCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTATGGCAGG‑CTG‑C‑CTTTTTA‑GCAGCCCGATATCTCCGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCGAACTAGCGGCTCTa > 6:234321/1‑139 (MQ=255) acaTACAACAAAACGTTTAATATAAAAACATCCCTCCGTTTTTTTTGGGCCGT‑CTG‑CTT‑TTTTC‑GACGCCGTTTTTTTCCGCCCCCGCCCCAAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 4:220454/137‑1 (MQ=255) aGATACAAGCAAAGTTTTCATTGAAAAACTGCCCACCGCTTCTTTTGGCCTGG‑CAG‑C‑CTTTTTA‑GCTGCGGTTTTTCACAGCCGCCGCCCGCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 8:54389/139‑1 (MQ=255) gaaaaaaGCACACGTTTAATACAAAAACAGCCCTCCGCTTCTTTTTGCCCGC‑CTG‑C‑CTTTTTC‑GCCGCCGTTTTTCTCCGCCCCACCCCCCAAGAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaa < 6:117983/134‑1 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTGGCAAACTAGCGTCTCTAGCAAAAc > 5:54903/1‑139 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGGGCTAGCaaaa > 2:290331/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTATCGGCTCAAGCaaaa > 3:220454/1‑138 (MQ=255) aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGGTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCATTTCTCGCAAACTAGCGGCGCTAGCaaaa > 5:318709/1‑138 (MQ=255) aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCCGCCGCAGCCCCAAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa < 4:284000/139‑1 (MQ=255) gCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCACCCCGCATGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:59620/139‑1 (MQ=255) ccAAACGTTTAATATAAAAACATCCCTCCGCTTATTTTTGGCCGG‑AAGAC‑T‑TTTTC‑GCAGCCCGTTTTCTACGCCGCAGCCCCAAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 6:157389/138‑1 (MQ=255) aaaGGTTTAATACAAAAACAGCCCCCCGCTTCTTTTGGGCCGG‑CCC‑C‑CTTTTTA‑CCCCCCGGTTTTCTCCGCCCCCCCCCGAAACAACCCACGGCCGATTTCTTTTCTCGCAAACTCGCGGCTCTAGCAAAACTCATGa < 1:316627/139‑1 (MQ=255) aaaCGTTTCATCTAAAAACAACCCTCGGTTTCTTTTTGGCCGC‑CTG‑CTT‑TTTTA‑GCCGCCCGTTTTCTCTGCCGCTGCCCGCAAGAATCGACGGCCGTTTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 4:153597/139‑1 (MQ=255) aaaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCCGCCCCCAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 7:206937/139‑1 (MQ=255) aaaCGTTTAATATAAAAACATCCCACCGATGCTTTTTGGCCGC‑CAG‑C‑CTTTTTA‑GCACCCCGTTTGCTCCGCCCCCCCCCCCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 3:157817/139‑1 (MQ=255) aaaCGTTCAATATAAAAACAACCCTCCGATTTTTTTGGGCTGC‑CAG‑CAT‑TTTTC‑CCAGCCGGTTTACACCGCCGCCGCCCGAAATAAAACACGGCCTATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 5:291930/139‑1 (MQ=255) ccGTTTAAAAAAAAAACATCCCTCCGTTTCTTTTGGGCTGC‑CCG‑CAT‑TTTTA‑GACGCCGTTTTTACCACCCGCCGCCCCAAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 6:195497/138‑1 (MQ=255) ccGTTACAAACAAAAACATCCCCCGGGTGCTTTTGGGCGGG‑CAG‑CGT‑TTTTC‑GCCGCCCGTTTGCCCCGCCGCCCCCCCAAAAAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 1:226990/138‑1 (MQ=255) acttttCATACAAAACCACCCCGCCGCTTATTTTGGCCCGG‑CCG‑C‑CTTTTTC‑GCCGCCGGTTTCCCCCGCCGCCCCCCGCAAAAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 1:42571/136‑1 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATTTCTTTTCTCGCAAACTAGCGGCGCGAGCCAAACTCATGAc > 7:123259/1‑138 (MQ=255) aCGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATTTCTTTTCTCGCAAACTAGCCGCGCAAGCAAAACACATgcc > 5:177339/1‑136 (MQ=255) aCGTTTCATACAAAAACATCCCTCCGATTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCCGCCCCAGCCCGAACTAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 8:257323/139‑1 (MQ=255) caatccaaaacCATCCGCCCGATTATTTTTGCCAGC‑CCT‑C‑T‑TTTTACGCAGCCCTTTTGCTCCGCCGACCCCCCCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATg < 4:290724/133‑1 (MQ=255) cATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CCG‑C‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGTTTTATCGCAAACTAGCCGCGCTAGCAAAACTCATGACGCTATGa > 4:122532/1‑139 (MQ=255) atatAAAAAAATCCGTCCGTTTTTTTTTGGATGGACGA‑T‑T‑TTTTA‑GCCCCCGTTTTTCTCACCCCCAGCCCCAAAAAAACCAGGGCCGATTTCTTTTCTCTCAAAGTAGCGGCTATAGCAAAACTCATGACTCTATGAt < 8:140267/139‑1 (MQ=255) aaaaCATCCCTCCGCTTATTTTTGGCCGG‑CAG‑C‑CGTTTTA‑GCATCCCGTTTTCTCAGCCCCACCCCGCAACAAAAGCCCGCCGATTTCTTATCGCCCAAACTAGCGGCGCTACCAAAAACCATCACACCATTATAGAcc > 4:183984/1‑139 (MQ=255) aaaCATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTTTTTA‑GCACCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAATTCAAGTCTCGCAAACTACCGGCTCTAGCAAAACTCATGACTCTATGATAGACCg > 5:130828/1‑139 (MQ=255) a‑gcagcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaaaaaa > 6:294405/1‑139 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTTGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAcc > 2:89381/1‑138 (MQ=255) agcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGTAAGAGAATTCTTTGTCACAAAAAGAGTGAAAAAAcc > 3:131201/1‑138 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGATTTATCGCAAACTTGCGGCTGGAGCAAATCTCATGGCTCTATGATAGCCCCGGGAAAGAGATTTCTTTGGGGGCATTTGAGTGaaaaaagcta > 4:63901/1‑135 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCa > 7:29698/1‑55 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCa < 8:29698/55‑1 (MQ=255) gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACGCATGTATCGATGATAGTCCGTGGTAAGAGAATTCTATGTCACAATATGTGTGAAAAAACCaa > 8:166851/1‑139 (MQ=255) cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAATAGAATTCTTTTTCACAATATGATTTAAAAAACCaa > 1:147010/1‑138 (MQ=255) cAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGtttt > 3:225632/1‑136 (MQ=255) aGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAATAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGTAAAGAGAATTCTTTGTCTAAATATGAGTGAAAAAACAAACTAGTTTTTAg > 6:99217/1‑138 (MQ=255) || TTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑C‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAG > CP006881/3725119‑3725363 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |