| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | BS168_reference_genome | 3,935,825 | Δ1 bp | coding (1190/1191 nt) | rlmI ← | large subunit ribosomal RNA m5C1962 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 3,935,823 | 0 | T | . | 99.6% | 1341.5 / ‑0.4 | 269 | intergenic (+35/+1) | ywbE/rlmI | conserved protein of unknown function/large subunit ribosomal RNA m5C1962 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (127/140); total (128/141) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
Only 100 of 292 total aligned reads displayed.
GACGAGAGGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCATTTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATAAAGCCTTTG > BS168_reference_genome/3935674‑3935968 | aCGAGAGGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGGTTTTTCatt > 1:3038733/1‑150 (MQ=255) cGAGAGGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCat < 1:3574948/148‑1 (MQ=255) gagaGGGACAGTGAAGGATATTTTGCCGAAATCAAACTTTCCACCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCattat < 2:1153181/150‑3 (MQ=255) gagaGGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCattat < 2:1577237/150‑3 (MQ=255) agagGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTACAGTCCGTCTTGAAGATGCCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCattatt < 2:613098/150‑4 (MQ=255) agagGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCattatt < 2:2092819/150‑4 (MQ=255) agagGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTCTCTACATAAAAAAAAGCTGCCCCTGCACAGGGCAGGGCTTTCattatt > 1:2322955/1‑147 (MQ=255) agGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTAtttt < 2:33634/150‑1 (MQ=255) aCAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCGTTTTCA‑TTATTTTTTCt > 1:2254834/1‑150 (MQ=255) gTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCa < 1:1597425/150‑1 (MQ=255) gTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTGTTCA‑TTACTTTATCTGCa > 1:834512/1‑150 (MQ=255) aaGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTTTTTTTTTTTTCTTCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGaa > 1:996529/1‑150 (MQ=255) aaGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGaa > 1:2495072/1‑150 (MQ=255) gATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTATTTCA‑TTATATTTTCTGCTGAAGGa > 1:2390138/1‑150 (MQ=255) atatTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGAc < 2:2741141/150‑1 (MQ=255) tatTTTGTCGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAAATCGTTTCTACATAAAAAAAAAGTGGCCCTGAACAGGGCAGCTTTTCCCTTT‑TTTTTTTTGCAGAAGGAcc > 1:2452697/1‑150 (MQ=255) tatTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGAcc < 1:1363954/150‑1 (MQ=255) atTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGGACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGGCCa > 1:1910833/1‑150 (MQ=255) ttttGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCCGAACAGGGCAGCTTTTTCA‑TTATTGTTTCTGCGGGAGGACCAc > 2:367482/1‑150 (MQ=255) tttGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACt > 2:2698237/1‑150 (MQ=255) tGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGAACGTTTCTACATAAAAAAAAGATGACCCTGTACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGAGCCACttt > 1:3485056/1‑150 (MQ=255) tGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCACATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGGAGGACCACttt > 1:2579932/1‑150 (MQ=255) tGACGAAACCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGTCCACttt < 1:3683804/150‑1 (MQ=255) tGACGAAAACAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACttt < 1:1209709/150‑1 (MQ=255) aCGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGCTGGCCGCATCGCCCGCGACCAGCAGATCGTTTCTACAGAGAAAAAAGCTGCCCCTGACCAGGGCAGCTCTTTCA‑TTATTTTTTCTGCAGAAGGACCCCTTTCa < 2:2242489/150‑1 (MQ=255) cgacctCAACCTTTCATCCGCATGGCAGTAAAGCCCGACTTGAAGATGGCCCCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAg < 2:2378140/145‑1 (MQ=255) aaaTCCAACTTTCATCCGCATGGCTTTAAAGCCCGCCTTGGAGATGGCCGCATCGGCCGCGACCAGCAGGTCGTTTCTACATAAAAAAATGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGt < 2:1726598/150‑1 (MQ=255) aaaTCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTTTGCAGAAGGACCACTTTCAGGt > 2:37689/1‑150 (MQ=255) aaaTCAAACTTTCACCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAAAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGt < 1:3973445/150‑1 (MQ=255) aTCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAg > 2:294944/1‑150 (MQ=255) aTCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAg < 2:1845423/150‑1 (MQ=255) aTCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGACCGTTTCTACATAAAAAAAATCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAg < 1:642317/150‑1 (MQ=255) cAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGtt > 2:2489690/1‑150 (MQ=255) aaaCTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTg < 2:3524422/150‑1 (MQ=255) accTTCCATCCGCATGGCATTAAAGTCCGTCTTGCAGATGGCCGCAACGGCCGCGCCCAGCAGATCGTTACTACATAAAAAAAACCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGc < 2:3684698/148‑1 (MQ=255) ccTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGACCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGcc < 2:2273974/149‑1 (MQ=255) ccTTCCATCCGCATGGCATTAAAGTCCGCCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGcc < 2:2866397/149‑1 (MQ=255) cTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCt > 2:3241622/1‑150 (MQ=255) tttCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCtt < 2:2787855/150‑1 (MQ=255) ttcttcCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGTTCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTc < 2:1321350/150‑1 (MQ=255) aTCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCCGGAGGACCACTTTCAGGTAGGTGCCTTCCgg > 2:458895/1‑150 (MQ=255) tCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGGAGGACCACTTTCAGGTAGTTGCCTTCCGGa > 1:1513645/1‑150 (MQ=255) tCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTGGTTGCCTTCCGGa > 1:908152/1‑150 (MQ=255) gCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGTACAGGGCAGCATTTTCA‑TTATTTTTTCTGCAGAAGGTCCACTTTCAGGTAGGTGCCTTCCCGAAAt > 1:3112763/1‑150 (MQ=255) gCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAAt > 2:3851838/1‑150 (MQ=255) cATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGTCCACTTTCAGGTAGTTGCCTTCCGGAAATg > 1:1430266/1‑150 (MQ=255) cATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTATCAGGTAGTTGCCTTCCGGAAATg > 1:1654959/1‑150 (MQ=255) tGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGcc > 2:1541546/1‑150 (MQ=255) ggCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCg > 2:3426769/1‑150 (MQ=255) cATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGaa > 2:3893838/1‑150 (MQ=255) tAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATc < 2:97827/150‑1 (MQ=255) aaGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGt < 2:1379268/150‑1 (MQ=255) aaGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCACCTTTTTCA‑TTATTTGTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGt < 1:433052/150‑1 (MQ=255) cGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGa < 2:2184003/150‑1 (MQ=255) tGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAAtcc > 1:338998/1‑150 (MQ=255) aaGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGATCAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAAtcctc < 2:2927185/150‑1 (MQ=255) gATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCg > 1:3207825/1‑150 (MQ=255) aTGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGATGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGGAATCCTCCgg > 1:3253259/1‑150 (MQ=255) aTGGCCCGATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCgg < 2:2535726/150‑1 (MQ=255) cGCATGGGCCGCGACCAGCAGATCTTTTCTACATAAAAATAAGCTGCCCCTGAACAGGGCATCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCg < 1:2773273/150‑1 (MQ=255) gCCGCGACCAGCAGATCGTTTCCACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTc < 2:1813667/150‑1 (MQ=255) gcgACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTc < 2:401322/150‑1 (MQ=255) gcgACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTc > 2:1643387/1‑150 (MQ=255) gACCAGCAGATCGTTTCTCCATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCaa < 1:3765485/150‑1 (MQ=255) ccAGCAGATCGTTTCTCCATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATg < 2:2994401/150‑1 (MQ=255) ccAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATg > 1:726795/1‑150 (MQ=255) cagcagATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGa > 2:3370359/1‑150 (MQ=255) agATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGATATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCgt < 1:2698237/150‑1 (MQ=255) agATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCgt > 2:195612/1‑150 (MQ=255) gATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCgtg > 2:2668032/1‑150 (MQ=255) aTCGTTTCTACATAAAAAAAAGCTGGCCCTGAACAGGCCATCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTCCAGGTAGTTGCCTCCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCgtgt < 1:3926757/150‑1 (MQ=255) aTCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGGAATCCTCCGGCAGCGGAAATTCCTCAATGATCgtgt > 2:2894397/1‑150 (MQ=255) tttCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCg < 1:3426769/150‑1 (MQ=255) cATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCAtt > 1:406837/1‑150 (MQ=255) aTAAACAAAAGCTGCCCCTGACCAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGAGCACTTTCAGGTAGTTGCCTGCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCAttt < 1:511090/150‑1 (MQ=255) aTAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCAttt < 2:937983/150‑1 (MQ=255) tAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTg < 2:1475486/150‑1 (MQ=255) aaaaaaaaGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGt > 1:2231679/1‑150 (MQ=255) aGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCTGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGGTTCTTTa > 2:2264088/1‑150 (MQ=255) gCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTaa > 1:1049024/1‑150 (MQ=255) gCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTaa < 2:3038733/150‑1 (MQ=255) cTGCCCCTGAACAGGGCAGCTGTTTCA‑TGATTGTTTCTGCAGAAGGACCCCTTTCAGGTAGGTGCCTTCCGGACATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTGAATTCGCCAATGATCGTGTAGCGTTCATTTGTTTCTTTaaa < 2:1550972/150‑1 (MQ=255) tGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAg < 1:2978403/150‑1 (MQ=255) ccTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggcgg < 2:3340998/150‑1 (MQ=255) cTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggcggc > 1:4005618/1‑150 (MQ=255) tGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCa > 2:807466/1‑150 (MQ=255) gAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCAt > 2:1110140/1‑150 (MQ=255) gAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGGAAGTTCCTCAATGACCGTGTAGGGTTCATTGGTTTCTTTAAAGGCTGCAt > 2:2215226/1‑150 (MQ=255) taCAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATc > 2:3104671/2‑150 (MQ=255) aaCAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTCGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATc > 1:2330842/1‑150 (MQ=255) cAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCaa < 1:1833394/150‑1 (MQ=255) cAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCaa > 1:1009081/1‑150 (MQ=255) gggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATa > 2:3627019/1‑150 (MQ=255) gtcAGCTTTTTCA‑TTACTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCAGGAAATGACGAAAATGTTTTGAAATCCTCCGGCAGCGTAAATTCCGCAATGATCGTGTCGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATaa < 2:1504426/148‑1 (MQ=255) ggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAGTCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTATAGGCGGCATCAATaa > 2:2576397/1‑150 (MQ=255) gCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATaaa < 2:2334875/150‑1 (MQ=255) aGCGTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTAGCCTACCGGAAATGCAGAAATCGTCTTGAAATCCCCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATAAAGc < 1:253465/150‑1 (MQ=255) tttttCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATAAAGCCtt < 2:3963656/150‑1 (MQ=255) ttttCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATAAAGCCttt < 2:1198558/150‑1 (MQ=255) ttttCA‑TTATTTTTCCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATAAAGCCttt < 2:529889/150‑1 (MQ=255) | GACGAGAGGGACAGTGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACATAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCATTTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCATCAATAAAGCCTTTG > BS168_reference_genome/3935674‑3935968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |