Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 2,387,885 (A)7→8 coding (704/735 nt) yphF ← putative lipoprotein of unknown function

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome2,387,8781.A94.3% 726.3 / 35.1 211coding (711/735 nt)yphFputative lipoprotein of unknown function
Reads supporting (aligned to +/- strand):  ref base . (5/7);  new base A (114/85);  total (119/92)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 235 total aligned reads displayed.

AACACAGGACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAAGAAGCCAGCTCAGT  >  BS168_reference_genome/2387729‑2388024
                                                                                                                                                      |                                                                                                                                                       
aaCACAGGACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCt                                                                                                                                                          <  1:138901/150‑1 (MQ=255)
aaCACAGGACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCt                                                                                                                                                          >  2:3054176/1‑150 (MQ=255)
aaCACAGGACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCt                                                                                                                                                          <  2:3819578/150‑1 (MQ=255)
   acaGGACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑a‑a‑a                                                                                                                                                   >  2:3547338/1‑150 (MQ=255)
      ggACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑a‑a‑a‑aaa                                                                                                                                               <  1:4049984/150‑1 (MQ=255)
      ggACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑a‑a‑a‑aaa                                                                                                                                               >  2:2148650/1‑150 (MQ=255)
       gACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATATCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑a‑a‑a‑aaa‑a                                                                                                                                             <  2:3988189/150‑1 (MQ=255)
        acacTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCAATCCTTTTTCATATCATTTTCT‑‑a‑a‑a‑aaa‑aa                                                                                                                                            >  1:1917322/1‑149 (MQ=255)
          acTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGGAAGGGAGAAGGGTTCCTTCCTTTTTCATATCCTTTTCT‑‑g‑a‑a‑aaagaca                                                                                                                                           >  1:690694/1‑146 (MQ=255)
           cTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑a‑a‑a‑aaa‑aacag                                                                                                                                         <  2:3718519/150‑5 (MQ=255)
           cTATATGTGTATGACGTTGTCCTATTTGAATATGACAACTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑a‑a‑a‑aaa‑aacag                                                                                                                                         <  2:1012345/150‑5 (MQ=255)
             gtatGTGCATGACGTTGTCCTATGTGGATAGGACACCTTTAGAAGATTGCTCACGCCCAACCTACTACTATGAAGCAACCTTTCTCCCTGTTTGTATGCAGGGGAGCAACCTTCATTCCTTTTTCATATCATTTTCT‑‑A‑A‑AGAAA‑GCAGGt                                                                                                                                        <  2:690694/149‑1 (MQ=255)
             atatGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGt                                                                                                                                        <  2:451949/150‑1 (MQ=255)
             atatGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGt                                                                                                                                        >  2:1402465/1‑150 (MQ=255)
                   gtATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGt                                                                                                                                  >  2:560350/1‑150 (MQ=255)
                    taagACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGtt                                                                                                                                 <  1:1904915/147‑1 (MQ=255)
                    tATGACGTTTTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGtt                                                                                                                                 <  1:1516284/150‑1 (MQ=255)
                         cGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ATAGGTTCCTGTTTGTCa                                                                                                                            <  1:3141259/150‑1 (MQ=255)
                          gTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCAt                                                                                                                           >  2:3903992/1‑150 (MQ=255)
                          gTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑A‑A‑A‑AAATACAGGTTCCTGTTTGTCAt                                                                                                                           >  1:357156/1‑150 (MQ=255)
                            tGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAACCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCAttt                                                                                                                         <  2:2692474/150‑1 (MQ=255)
                             gTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTa                                                                                                                        <  2:1714434/150‑1 (MQ=255)
                             gTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAAACTTTCTCCCTATTTGTATGCAAGGGGGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTTGCATTTa                                                                                                                        >  1:3668827/1‑150 (MQ=255)
                              tCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTTTGCAAGGGAGAAAGGTTCATTCCTTTTTTATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGGTCCTGTTTGGCTTTTAc                                                                                                                       >  1:2251382/1‑150 (MQ=255)
                                     tGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTatat                                                                                                                >  1:2773788/1‑150 (MQ=255)
                                      gAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATAtt                                                                                                               >  2:2362406/1‑150 (MQ=255)
                                       aaTATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATAttt                                                                                                              <  2:3207107/150‑1 (MQ=255)
                                          atGCCATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAA‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTa                                                                                                            <  2:3479017/150‑1 (MQ=255)
                                           tGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACa                                                                                                          <  1:1011011/150‑1 (MQ=255)
                                           tGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCAATTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACa                                                                                                          >  1:1257309/1‑150 (MQ=255)
                                            gACATCTTTAGTAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGGTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAg                                                                                                         >  2:1463676/1‑150 (MQ=255)
                                            gACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAg                                                                                                         >  2:1347929/1‑150 (MQ=255)
                                            gACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑A‑ATA‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAg                                                                                                         >  1:1111031/1‑150 (MQ=255)
                                             aCATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGa                                                                                                        <  1:2084268/150‑1 (MQ=255)
                                              cATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACTCTTCTTCCTATGTGTATGCAAGGGAGGAAAGATCATTCCTTTTTCTTATTATTTTCT‑‑AGA‑A‑AAA‑ACAGGTTACTGCTTGTCATTTACCGTATCTTTTActgca                                                                                                       >  1:2545567/1‑146 (MQ=255)
                                              cATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGaa                                                                                                       >  2:75272/1‑150 (MQ=255)
                                                tCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGaaaa                                                                                                     >  2:95480/1‑150 (MQ=255)
                                                 cTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAc                                                                                                    <  1:2521911/150‑1 (MQ=255)
                                                     aGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCgg                                                                                                <  2:2107656/150‑1 (MQ=255)
                                                     aGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCgg                                                                                                <  1:32372/150‑1 (MQ=255)
                                                      gAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCggg                                                                                               >  2:904712/1‑150 (MQ=255)
                                                       aaaaTTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCgggg                                                                                              >  2:3583077/1‑150 (MQ=255)
                                                        aaaTTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgtttaaa                                                                                             <  2:1111031/150‑7 (MQ=255)
                                                        aaaTTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGa                                                                                             <  2:2773788/150‑1 (MQ=255)
                                                         aaTTTCCCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGAc                                                                                            <  1:973273/150‑1 (MQ=255)
                                                          aTTTCTCATCCTCTATCTATTTATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACa                                                                                           <  1:750604/150‑1 (MQ=255)
                                                              ctcATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAGGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAgg                                                                                       <  1:1667994/150‑1 (MQ=255)
                                                                 aTCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAggggg                                                                                    <  2:223132/150‑1 (MQ=255)
                                                                  tCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGt                                                                                   <  1:2818484/150‑1 (MQ=255)
                                                                    cTCAATCTATTAATCTAAAAAATCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGttt                                                                                 <  1:2989797/150‑1 (MQ=255)
                                                                    cTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGttt                                                                                 <  2:3448866/150‑1 (MQ=255)
                                                                      cAATCTATTAATATAAAAAAACTTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCa                                                                               >  1:2185230/1‑150 (MQ=255)
                                                                          cTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGcc                                                                           >  2:3792550/1‑150 (MQ=255)
                                                                             ttAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCaaa                                                                        <  1:1092069/150‑1 (MQ=255)
                                                                             ttAATATAAAAAAAACTTTCTCCCTATTTGTAAGCAAGGGAGAAAGGTTCATTCCTTTCTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCaaa                                                                        <  1:3013283/150‑1 (MQ=255)
                                                                              tAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAAt                                                                       >  2:2330125/1‑150 (MQ=255)
                                                                                atatAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCa                                                                     >  1:1602278/1‑150 (MQ=255)
                                                                                    aaaaaaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATc                                                                 >  2:323341/1‑150 (MQ=255)
                                                                                        aaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTgg                                                             >  2:3322765/1‑150 (MQ=255)
                                                                                         aaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTATCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGa                                                            >  1:1697849/1‑150 (MQ=255)
                                                                                         aaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGa                                                            <  1:90448/150‑1 (MQ=255)
                                                                                         aaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGa                                                            >  2:2014489/1‑150 (MQ=255)
                                                                                              ttCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCa                                                       >  1:3441051/1‑150 (MQ=255)
                                                                                              ttCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCa                                                       >  1:3637453/1‑150 (MQ=255)
                                                                                                ctcCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGt                                                     >  2:758010/1‑150 (MQ=255)
                                                                                                ctcCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGt                                                     <  1:2428917/150‑1 (MQ=255)
                                                                                                ctcCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGt                                                     <  2:2919190/150‑1 (MQ=255)
                                                                                                  cccTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTATCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTcc                                                   >  1:1621258/1‑150 (MQ=255)
                                                                                                     tATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCgg                                                >  2:308481/1‑150 (MQ=255)
                                                                                                     tATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCgg                                                >  2:1561613/1‑150 (MQ=255)
                                                                                                        ttGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCCTATATTTTACAGAAAACGCGGGGACAAAGGGGGGTTCATGCCAAATCTAATCCTGGATTTCAGTCCCGGGtt                                             >  2:3805955/1‑150 (MQ=255)
                                                                                                          gTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTGTATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTa                                           >  2:3385835/1‑150 (MQ=255)
                                                                                                             tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAAc                                        >  1:831996/1‑150 (MQ=255)
                                                                                                             tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAAc                                        >  1:578241/1‑150 (MQ=255)
                                                                                                             tGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAAc                                        <  1:4034466/150‑1 (MQ=255)
                                                                                                               cAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGt                                      <  1:3276682/150‑1 (MQ=255)
                                                                                                                aaGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCAGGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACggt                                     >  2:1968150/1‑148 (MQ=255)
                                                                                                                    gagaAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATTCTGGATTTCAGTCCCGGGTTTAAACGtttttt                                 <  1:2418553/150‑1 (MQ=255)
                                                                                                                     agaAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGttttttt                                >  2:2546300/1‑150 (MQ=255)
                                                                                                                      gaAAGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGttttt                                  <  1:3305933/147‑1 (MQ=255)
                                                                                                                       aaaGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGc                              <  1:3492751/150‑1 (MQ=255)
                                                                                                                        aaGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCt                             <  1:1097741/150‑1 (MQ=255)
                                                                                                                         aGGTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCtt                            <  1:968368/150‑1 (MQ=255)
                                                                                                                          ggTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCttt                           <  2:1912515/150‑1 (MQ=255)
                                                                                                                           gTTCATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCtttt                          >  2:1211636/1‑150 (MQ=255)
                                                                                                                            tccATTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCttttt                         <  2:3637453/148‑1 (MQ=255)
                                                                                                                               aTTCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTg                      <  2:2385082/150‑1 (MQ=255)
                                                                                                                                 tCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAg                    <  1:2571495/150‑1 (MQ=255)
                                                                                                                                 tCCTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAg                    >  2:508631/1‑150 (MQ=255)
                                                                                                                                  ccTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAgg                   >  2:2640499/1‑150 (MQ=255)
                                                                                                                                  ccTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAgg                   >  2:327542/1‑150 (MQ=255)
                                                                                                                                   cTTTTTCATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGc                  <  1:2754239/150‑1 (MQ=255)
                                                                                                                                         cATATCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCaagaag            >  1:1434166/1‑150 (MQ=255)
                                                                                                                                            atCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGGTAAAACGTTTTTTGGCTTTTTTTGGGGCAAGAAGCCa         >  2:2705729/1‑150 (MQ=255)
                                                                                                                                             tCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGGGGCAAGGAGCCAg        >  2:317553/1‑150 (MQ=255)
                                                                                                                                             tCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAAGAAGCCAg        <  1:1861888/150‑1 (MQ=255)
                                                                                                                                             tCATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAAGAAGCCAg        >  1:3805223/1‑150 (MQ=255)
                                                                                                                                              cATTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTATTTTTGCTTTATTTGAGGCAAGAAGCCAGc       >  2:1070228/1‑150 (MQ=255)
                                                                                                                                               aTTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAAGAAGCCAGCt      <  1:90513/150‑1 (MQ=255)
                                                                                                                                               aTTTTCTA‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAAGAAGCCAGCt      >  2:624683/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
AACACAGGACACTATATGTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑‑A‑A‑A‑AAA‑ACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAAGAAGCCAGCTCAGT  >  BS168_reference_genome/2387729‑2388024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑