BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	165,826	+C	intergenic (+1/‑4)	trnI‑Asn → / → trnI‑Thr	tRNA‑Asn/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,825	1	.	C	100.0%	678.7 / NA	200	noncoding (72/72 nt)	trnI‑Asn	tRNA‑Asn
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (89/111); total (89/111)

Only 100 of 200 total aligned reads displayed.

GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTA  >  BS168_reference_genome/165693‑165967

ggACGCCGGCAAGCAGATTTATTAATTCTATCATGTTTGTTTATGAAGGTTTATAT‑T‑ATTCCGCAGTAGCCCAGTGGTAGAGCTATCGGCTGTTGACCGATCGGACGTAGGTTCGAATCCTAGCTGCGGAGCCATTGCTTCCATAGcaca                                                                                                                              <  1:3350701/150‑1 (MQ=18)

     ccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                                                                                                                         <  2:867123/150‑1 (MQ=21)

     ccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                                                                                                                         <  2:1589837/150‑1 (MQ=21)

     ccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                                                                                                                         <  1:3220390/150‑1 (MQ=21)

      cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgga                                                                                                                        >  2:2004387/1‑149 (MQ=21)

      cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGt                                                                                                                        <  1:3420709/150‑1 (MQ=21)

       gccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTa                                                                                                                       >  1:3612940/1‑150 (MQ=21)

       gccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTa                                                                                                                       <  2:260530/150‑1 (MQ=21)

        ccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTag                                                                                                                      <  2:2345110/150‑1 (MQ=21)

         cAAGCAGATTTTTTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTCCCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTaga                                                                                                                     <  2:1063510/150‑1 (MQ=21)

           aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGc                                                                                                                   >  2:3311605/1‑150 (MQ=21)

            gCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTGTAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCa                                                                                                                  <  2:2981565/150‑1 (MQ=21)

            gCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCa                                                                                                                  <  2:3882219/150‑1 (MQ=21)

               gATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCCCtt                                                                                                               >  2:3040631/1‑150 (MQ=21)

               gATTTATTAATTCTACCATGTTTGTTTATGAAGCTT‑‑TATCTTATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                               <  2:2974160/150‑1 (MQ=21)

                aTTTATTACTTCTACCATGTCTGTTTATGAAGCTTTATAT‑T‑ACTCCGCAGTAGCCCAGTGGTAGAGCTATCGGCTGTTACCCGATCGGTCGAAGGTTCGCATCCCACCTGCGGAGCCCTTGCTCCCATAGCTCAGCAGGTAGAGCACTTc                                                                                                              <  2:4033190/150‑1 (MQ=18)

                aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                                                                                                              >  2:52319/1‑150 (MQ=21)

                aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                                                                                                              <  2:800981/150‑1 (MQ=21)

                aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                                                                                                              >  2:734793/1‑150 (MQ=21)

                aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                                                                                                              <  1:2892004/150‑1 (MQ=21)

                   tattaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCAt                                                                                                           >  1:937201/1‑150 (MQ=21)

                    attaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGGGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATg                                                                                                          >  1:1704390/1‑150 (MQ=21)

                      taATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGt                                                                                                        >  1:2943756/1‑150 (MQ=21)

                       aaTTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa                                                                                                       >  2:2411630/1‑150 (MQ=21)

                       aaTTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa                                                                                                       >  1:1965252/1‑150 (MQ=21)

                        aTTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTaa                                                                                                      <  2:3964979/150‑1 (MQ=21)

                        aTTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGAAGGTTCGAATCCTACCTGCGGAGCCATTGCTACCATAGCTCAGCAGGTAGAGCACTTCCTAGGTaa                                                                                                      >  1:2849838/1‑150 (MQ=21)

                         ttCTACCATTTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCCCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAg                                                                                                     <  1:3618025/150‑1 (MQ=21)

                         ttCTACCATGTTTGTTTATGAAGCTTTATAT‑TC‑TTCCGCAGTACCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAg                                                                                                     <  1:3960128/150‑1 (MQ=21)

                          tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCAAAGCTCACCAGGGAGGGCACTTCCATGGTAAgg                                                                                                    >  1:2998180/1‑150 (MQ=18)

                           cTACCATGTGTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCCCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGCCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGa                                                                                                   <  1:2120702/150‑1 (MQ=21)

                            tACCGTGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTTACCGATCGGTGGTAGGTTCGAATCCTCCCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAggta                                                                                                  <  2:2657071/150‑3 (MQ=21)

                            tACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                                  <  2:3381869/150‑1 (MQ=21)

                              ccATGTTTGTTTATGAAGCTTTATAT‑T‑GTTCCGCAGTAGCTCAGTGGTAGAGCTATGGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAaga                                                                                                <  1:3821161/150‑1 (MQ=21)

                                aTGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAgg                                                                                              <  2:1350847/150‑1 (MQ=21)

                                 tgtttgttTATGAAGCTTTATAT‑T‑ATTCCGCATTAGCCCAGTGGTAGAGTTATTGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGt                                                                                             <  1:1283638/150‑1 (MQ=21)

                                 tgtttgttTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGt                                                                                             <  2:2224317/150‑1 (MQ=21)

                                  gtttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTc                                                                                            <  2:3746638/150‑1 (MQ=21)

                                  gtttgtttAGGAAGCTTTATAT‑T‑ATTCCGCAGTAGCCCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATGCTACCTGGGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACGTCCATGGTAAGGAAGAGGTc                                                                                            <  1:2196140/150‑1 (MQ=21)

                                   tttgtttATGAAGCTTTATAT‑T‑ATCCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCa                                                                                           <  2:668056/150‑1 (MQ=21)

                                    ttgtttgtGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAg                                                                                          <  1:2628607/150‑1 (MQ=21)

                                    ttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAg                                                                                          <  2:216719/150‑1 (MQ=21)

                                      gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGGGGTCAGCg                                                                                        >  2:1315505/1‑150 (MQ=21)

                                      gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCg                                                                                        <  1:879350/150‑1 (MQ=21)

                                      gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGCAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCg                                                                                        >  1:2484009/1‑150 (MQ=21)

                                        ttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCGGTTACCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGt                                                                                      <  2:2500121/150‑1 (MQ=21)

                                         tATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGtt                                                                                     <  1:2677742/150‑1 (MQ=21)

                                         tATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGtt                                                                                     >  2:1268731/1‑150 (MQ=21)

                                         tATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGtt                                                                                     >  2:1799155/1‑150 (MQ=21)

                                          aTGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCCTAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTc                                                                                    <  2:173256/150‑1 (MQ=21)

                                           tGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCg                                                                                   <  2:1649073/150‑1 (MQ=21)

                                            gAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGa                                                                                  <  1:3072927/150‑1 (MQ=21)

                                             aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCTTAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAg                                                                                 >  1:1100360/1‑150 (MQ=21)

                                             aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAg                                                                                 >  2:2389028/1‑150 (MQ=21)

                                              aGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGGGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGc                                                                                >  1:71426/1‑150 (MQ=21)

                                                      atattaTTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGGTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAACAGGTCAGCGGTTCGAGCCCGCtt                                                                          >  1:1066729/6‑150 (MQ=25)

                                                        attaTTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                                        <  2:3422845/147‑1 (MQ=255)

                                                        attaTTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                                        >  1:193464/4‑150 (MQ=255)

                                                          taTTCCGCAGTAGCTCAGTGGTAGAGCCATCGGCTGTTACCCGGTCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCTCGCTTGGaa                                                                      <  1:1408757/149‑1 (MQ=25)

                                                             tCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCt                                                                   <  2:325620/150‑1 (MQ=255)

                                                               cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTa                                                                 <  2:1046898/150‑1 (MQ=255)

                                                               cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTa                                                                 >  1:1847914/1‑150 (MQ=255)

                                                                 cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGTTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                               >  1:449892/1‑150 (MQ=255)

                                                                 cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                               <  2:224922/150‑1 (MQ=255)

                                                                 cAGTAGCTCAGTGCTAGAGCAATCGGCTGTTTACCGATCGGTCGTCGGGTCGAATCCTACCGGCGGAGCCATTGCTTCCGTAGCCCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGCCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                               <  2:3136563/150‑1 (MQ=255)

                                                                   gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGGGCCATTGCTTCCCTAGCTCCGCAGGAAGCGCACTGCCACGGTAAGGCAGAGGTCAGCGGTTCGAGCCCGCTTGGGAGCTTAAATg                                                             >  2:3898056/1‑150 (MQ=21)

                                                                      gCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGCATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGGGCACTTCCATGGTAAGGAAGAGGTCAGCGGGTCGAGCCCGCTTGGAAGCTCAAGTGgat                                                          >  1:2691178/1‑150 (MQ=25)

                                                                       cTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtatt                                                         <  2:3799017/150‑1 (MQ=255)

                                                                           gTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                                     <  2:2054753/150‑1 (MQ=255)

                                                                              gTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCa                                                  <  2:899567/150‑1 (MQ=255)

                                                                               tAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCaa                                                 >  2:2471617/1‑150 (MQ=255)

                                                                               tAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCaa                                                 >  1:498761/1‑150 (MQ=255)

                                                                               tAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCaa                                                 >  1:2347641/1‑150 (MQ=255)

                                                                               tAGAGCTACCGGCTGTTAACCGGTCGGCCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCaa                                                 <  2:3534790/150‑1 (MQ=38)

                                                                                agagCTCTCGGCTGTTACCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCCCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAg                                                <  2:3361402/150‑1 (MQ=38)

                                                                                agagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGGGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAg                                                >  1:3980911/1‑150 (MQ=255)

                                                                                agagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGGGCACTTCCATGGTAAGGAAGGGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAg                                                >  2:1413935/1‑150 (MQ=255)

                                                                                agagCTATCGGCTGGTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGACCGCTTGGAAGCTTAAATGTATTATTACCAAg                                                >  1:606235/1‑150 (MQ=255)

                                                                                  agCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                                              <  2:78277/150‑1 (MQ=255)

                                                                                   gCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGtt                                             <  2:953089/150‑1 (MQ=255)

                                                                                    cTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGttt                                            >  1:1198462/1‑150 (MQ=255)

                                                                                    cTATCGGCTGTTAACCGATCGGACGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGttt                                            >  1:3571822/1‑150 (MQ=255)

                                                                                     tATCGGCTGTTAACCGATCGGTCGTAGGTTCGAGTCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTtc                                           >  1:1236450/1‑150 (MQ=255)

                                                                                     tATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTtc                                           <  2:2867126/150‑1 (MQ=255)

                                                                                         ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                                       <  2:3212878/150‑1 (MQ=255)

                                                                                                aaCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                                <  2:3635835/150‑1 (MQ=255)

                                                                                                aaCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                                <  2:2127890/150‑1 (MQ=255)

                                                                                                 aCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGaa                               >  2:3664687/1‑150 (MQ=255)

                                                                                                       cGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttt                         >  2:1011385/1‑150 (MQ=255)

                                                                                                            gTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTa                    >  1:845891/1‑150 (MQ=255)

                                                                                                              aGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTAtt                  <  2:937201/150‑1 (MQ=255)

                                                                                                               ggTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTg                 >  2:1034909/1‑150 (MQ=255)

                                                                                                                gTTCGAATCCTACCTGGGGAGCCTTTGCATCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTACTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGc                <  2:1945635/150‑1 (MQ=255)

                                                                                                                 ttCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg               <  2:3612940/150‑1 (MQ=255)

                                                                                                                 ttCGAAGCCTCCCTGCGGAGCCATTGCTCCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg               <  2:1701466/150‑1 (MQ=255)

                                                                                                                   cGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTATGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGat             >  1:133404/1‑150 (MQ=255)

                                                                                                                     aaTCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCTGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGTAAGCTTTTTTTATTGCGagat           >  1:2046135/1‑150 (MQ=255)

                                                                                                                     aaTCCTACCTGCGGAGCCATTGCTCCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGatat           <  2:120092/150‑1 (MQ=255)

                                                                                                                      tcccTCCCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATg          <  2:3155021/148‑1 (MQ=255)

                                                                                                                          tACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGa      >  2:1548876/1‑150 (MQ=255)

GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTA  >  BS168_reference_genome/165693‑165967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`