BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,049	C→T	intergenic (+318/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,049	0	C	T	96.8%	18500.6 / NA	5576	intergenic (+318/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base C (5/170); new base T (3189/2178); total (3211/2365)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5659 total aligned reads displayed.

TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGA  >  pSIJ663_pHT315_araE_xy/4902‑5198

  gagaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttcttc                                                                                                                                                   <  2:3853516/150‑1 (MQ=255)

   agaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTtcttct                                                                                                                                                  <  2:2443307/150‑1 (MQ=255)

    gaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTa                                                                                                                                                 <  1:3604270/150‑1 (MQ=255)

      tCCTTTTTCTCTGCGCGTAACCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgt                                                                                                                                               <  2:791839/150‑1 (MQ=255)

        ttttttttCTGCGCGTAATATGCTGCTTGCAAACAAAAAAACCACCCCTACCACCGGTTGGTTGCTTGCCGGATCAAAGCCTACCAACTCTTTTGCCGGAGGTAACTGGCTTCCGCTTGGCGCAAGAACCAAAATCTGTTCCTCTAgtgc                                                                                                                                             >  2:1530491/2‑149 (MQ=255)

        cTTTTTTGCTGCGCGTCATCTGCTGCTTGCAAACAAAAAAGCCACCGCTACGAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtgt                                                                                                                                             <  1:2748280/150‑1 (MQ=255)

          ttttttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCCCAGATACCAAATACTGTTCTTCTAggggag                                                                                                                                           >  1:2816871/1‑145 (MQ=255)

            ttttCTGCGCGTAACCTGCTGCCTGCAAACAAAAAACCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                                         <  2:2529587/150‑1 (MQ=255)

             tttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCg                                                                                                                                        >  1:2175571/1‑150 (MQ=255)

               tCTGGGCGTAACCTGCTGCTAGCAGACAAAAAACCCACCGCTAGCAGGGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTa                                                                                                                                      <  1:918577/150‑1 (MQ=255)

                cTGCGCGTAATCTGCTGCTTGCAAACAAAAAAGCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAg                                                                                                                                     <  1:720920/150‑1 (MQ=255)

                 tGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTTGTGTAGCCGTAGt                                                                                                                                    >  1:3402797/1‑150 (MQ=255)

                  gcgcGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGtt                                                                                                                                   >  1:857440/1‑150 (MQ=255)

                   cgcgTAATCTGCTGCTTGCAAACAAAAAAACCATCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGtt                                                                                                                                   <  2:3093431/149‑1 (MQ=255)

                   cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGGTa                                                                                                                                  >  1:1398463/1‑150 (MQ=255)

                   cgcgTAACCTGCTGCATGAAAACAAAAAACCCCCCGCTACCAGCGTTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTTGTGTAGCCGTAGTTa                                                                                                                                  <  1:2410212/150‑1 (MQ=255)

                     cgTAACCCGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTCCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAgg                                                                                                                                <  2:3792533/150‑1 (MQ=255)

                      gTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGc                                                                                                                               >  1:2601129/1‑150 (MQ=255)

                      gTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGc                                                                                                                               >  2:709569/1‑150 (MQ=255)

                      gTAAGCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGGGGTTTGTTTGCCGGATCAAGAGCTGCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGATCTTCTAGTGGAGCCGTAGTTAGGc                                                                                                                               >  2:2118081/1‑150 (MQ=255)

                       tatcctcctgctTGCAAACAAAAAATCCACCGGTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCACCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcc                                                                                                                              <  2:3188449/143‑1 (MQ=255)

                       tAATCTGCTGCTTGCAAACAAAAAAAGCACCGCTACCAGCGGCGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCCGCAGTGCGCAGGTACCAAAATCTGTTCTTCTAGTGTAGCCGTAGTTAGGcc                                                                                                                              >  1:2330487/1‑150 (MQ=255)

                        aaTCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTTGGcca                                                                                                                             >  1:650313/1‑150 (MQ=255)

                         acctgctgCTTGCACACAAAACACCCCGCGCTACCAGCGGTGGTTTGTTTGCCGGACCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                                            <  2:2700515/148‑1 (MQ=255)

                          tCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTTGTGTAGCCGTAGTTAGGccacc                                                                                                                           >  1:2673978/1‑150 (MQ=255)

                            tgctgcTTGCAAACAAACAACCCCCCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTGGTTAGGCcaccac                                                                                                                         <  2:3014515/150‑1 (MQ=255)

                               tgctTGCAAACAAAAACACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc                                                                                                                      <  1:1475341/150‑1 (MQ=255)

                               tgctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc                                                                                                                      >  2:3397672/1‑150 (MQ=255)

                               tgctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACCTc                                                                                                                      >  2:401454/1‑150 (MQ=255)

                                 ctTGCAAACAAAAAAAACACCGCTACCAGCGGTGGTTTGTTTGCCGGATCGAGAGCTACCAACTCTTTTTCCGTAGGTAACTGGCTTCAGCAGCGCGCAGATACCAAATACTGTTCTTCTAGGGTAGCCGGTGGTAGGCCACCACTTCaa                                                                                                                    >  2:1129355/1‑150 (MQ=255)

                                  ttccaaacaaaAACACCTCCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                                   <  1:796311/147‑1 (MQ=255)

                                  ttGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                                   >  2:87741/1‑150 (MQ=255)

                                  ttGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                                   >  1:1549806/1‑150 (MQ=255)

                                     caaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCCCTTCAAGAAc                                                                                                                >  1:3041597/1‑150 (MQ=255)

                                      aaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGCAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAct                                                                                                               >  2:2039468/1‑150 (MQ=255)

                                         caaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTg                                                                                                            >  2:1689082/1‑150 (MQ=255)

                                          aaaaaaaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTgg                                                                                                           >  1:2034675/1‑149 (MQ=255)

                                           aaaaacCCACCGCTACCAGGGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTa                                                                                                          <  2:2371549/150‑1 (MQ=255)

                                           aaaaaaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATTCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACGTCAAGGACTCTGTa                                                                                                          >  1:121242/1‑150 (MQ=255)

                                            aaaccccaccCCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                                         <  2:2932629/145‑1 (MQ=255)

                                             aacaccaccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGc                                                                                                        <  2:1054789/147‑1 (MQ=255)

                                               aaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc                                                                                                      >  1:247603/1‑150 (MQ=255)

                                                accaccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                                     >  1:3882895/1‑150 (MQ=255)

                                                accaccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGGAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                                     >  1:3538606/1‑150 (MQ=255)

                                                accaccGCTACCAGCGGTGATTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAcc                                                                                                     >  1:2275384/1‑150 (MQ=255)

                                                   accGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGGTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCCAGGACTCTGTAGCACCGcc                                                                                                  >  1:3948047/1‑150 (MQ=255)

                                                   accGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACCTCAAGAACTCTGTTGCACCGcc                                                                                                  >  1:980859/1‑150 (MQ=255)

                                                     cGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCCGATACCAAATACTGTTCTTCTAGTGTGGCCCTAGTTTGGCCACCACTTCTAGAACTCTGTAGCACCGCCTa                                                                                                >  1:2095957/1‑150 (MQ=255)

                                                      gCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAGGGTAACTGGCTTCAGCCGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTCCAAGAACTCTGTAGCACCGCCTAc                                                                                               >  1:3737713/1‑150 (MQ=255)

                                                      gCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAc                                                                                               >  2:3257669/1‑150 (MQ=255)

                                                       cTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCTGCAGAGCGCAGATACCAAATACTGATCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACa                                                                                              >  1:1749733/1‑150 (MQ=255)

                                                        tACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAt                                                                                             >  2:1907555/1‑150 (MQ=255)

                                                         aCCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGGGTAGCCGAAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATa                                                                                            >  1:2963325/1‑150 (MQ=255)

                                                          ccAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAc                                                                                           >  2:3533666/1‑150 (MQ=255)

                                                           cAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc                                                                                          >  2:1471888/1‑150 (MQ=255)

                                                            aGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCt                                                                                         >  1:500509/1‑150 (MQ=255)

                                                            aGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCt                                                                                         >  2:2850870/1‑150 (MQ=255)

                                                             gCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTCCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATCCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTc                                                                                        <  2:1605869/150‑1 (MQ=255)

                                                               ggtggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                                      >  2:3334777/1‑150 (MQ=255)

                                                                gtggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGct                                                                                     >  1:2526995/1‑150 (MQ=255)

                                                                  ggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGGAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctct                                                                                   >  1:3680677/1‑150 (MQ=255)

                                                                   gtttgtttGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTg                                                                                  >  1:1631043/1‑150 (MQ=255)

                                                                   gtttgtttGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCAAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTg                                                                                  <  2:1756020/150‑1 (MQ=255)

                                                                           gCCGGATCAAGGTCTCCCAACTCTTTTTCCGAAGGTATCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCt                                                                          <  2:2272810/150‑1 (MQ=255)

                                                                                 tCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAcc                                                                    >  1:429851/1‑150 (MQ=255)

                                                                                    agagCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGt                                                                 >  2:3471660/1‑150 (MQ=255)

                                                                                        cTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATTCCTCGCTCTGCTAATCCTGTTACCAGTGgct                                                             >  1:581339/1‑150 (MQ=255)

                                                                                          aCCAACTCTTTTTCCGAAGGTACCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgctgc                                                           <  2:1825837/150‑1 (MQ=255)

                                                                                             aaCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGGTACCAGGGGCtgctgc                                                        >  1:1324636/1‑150 (MQ=255)

                                                                                              aCTCTTTTTCCGAAGGTCACTGGCTACAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGcc                                                       <  1:1810603/150‑1 (MQ=255)

                                                                                              aCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGcc                                                       >  1:1059426/1‑150 (MQ=255)

                                                                                              aCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGcc                                                       <  1:1671763/150‑1 (MQ=255)

                                                                                              aCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGcc                                                       <  1:3242672/150‑1 (MQ=255)

                                                                                               ctctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCa                                                      >  1:1000372/1‑150 (MQ=255)

                                                                                                tctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGGCAg                                                     >  1:3808875/1‑150 (MQ=255)

                                                                                                tctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAg                                                     >  1:12675/1‑150 (MQ=255)

                                                                                                     ttCCGAAGGTACCTGGCTCCAGCAGAGCGCAGATACCAATTACTGTCCTTCTAGTGTAGCCGTAGTTCGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCg                                                <  2:373036/150‑1 (MQ=255)

                                                                                                     tcccGAAGGTACCTGGCTCCAGCAGAGCGCAGATCCCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCCCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCg                                                <  2:940751/148‑1 (MQ=255)

                                                                                                      tCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGa                                               <  2:1360498/150‑1 (MQ=255)

                                                                                                       ccGAAGGTAACTGGCTTCAGCAGAGCGCAGATCCCAAATCCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAt                                              <  1:1145094/150‑1 (MQ=255)

                                                                                                            ggTAACTGGCTCCAGCAGAGCCCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTc                                         <  2:603928/150‑1 (MQ=255)

                                                                                                              taccTGGCTCCAGCAGAGCGCAGATACCAATTCCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGACCTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgt                                       <  2:422816/147‑1 (MQ=255)

                                                                                                                   ggCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCtt                                  <  2:1271877/150‑1 (MQ=255)

                                                                                                                   ggCTACAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTTGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCtt                                  <  1:3112943/150‑1 (MQ=255)

                                                                                                                        cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTGAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGGCGGGTCCTACCgg                             >  2:2207545/1‑150 (MQ=255)

                                                                                                                         agcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTTGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCggg                            >  1:1887982/1‑150 (MQ=255)

                                                                                                                         agcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCggg                            >  2:1190364/1‑150 (MQ=255)

                                                                                                                           cagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGtt                          >  2:2787220/1‑150 (MQ=255)

                                                                                                                                 gcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGACGTGTCTTACCCGGTTGGACTc                    >  1:402777/1‑150 (MQ=255)

                                                                                                                                     atcccAAATACTGTTCTTCTAGGGTGGCCGTAGTTAGCCCCCCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGa                <  1:3181565/147‑1 (MQ=255)

                                                                                                                                     aTACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGa                >  2:510376/1‑150 (MQ=255)

                                                                                                                                         cAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTCATCCTGCTACCAGTGGCTGCTGCCAGTGGCGGTAAGTCGTGACTTACTGGGGTGGACTCAAGACGAt            >  2:1958461/1‑150 (MQ=255)

                                                                                                                                         cAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAt            >  2:3934349/1‑150 (MQ=255)

                                                                                                                                         cAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAt            <  2:2617019/150‑1 (MQ=255)

                                                                                                                                          aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTTGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATa           >  1:3316792/1‑150 (MQ=255)

                                                                                                                                             tccTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGtt        <  2:3661644/148‑1 (MQ=255)

                                                                                                                                              aCTGTCCTCCTAGTGTAGCCGTAGTTGGGCCACCACTTCAAGATCTCTGTAGCCCCGCCTACATCCCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTa       <  1:1968387/150‑1 (MQ=255)

                                                                                                                                               cTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCCCTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAc      <  1:2896464/150‑1 (MQ=255)

                                                                                                                                                tGTTCTTCTGGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAcc     <  1:3471660/150‑1 (MQ=255)

                                                                                                                                                tGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGACCTCTGTAGCACCGCCTACATCCCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAcc     <  2:3584591/150‑1 (MQ=255)

TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGA  >  pSIJ663_pHT315_araE_xy/4902‑5198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`