Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,317,152	+GT	intergenic (+23/+5)	phrA → / ← yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,317,152	1	.	G	97.3%	274.0 / ‑4.2	75	intergenic (+23/+5)	phrA/yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base G (33/39);  minor base T (0/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	BS168_reference_genome	1,317,152	2	.	T	97.3%	225.6 / NA	75	intergenic (+23/+5)	phrA/yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base T (33/39);  minor base A (0/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑G‑‑C‑TTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTC  >  BS168_reference_genome/1317017‑1317275                                                                                                                                           ||                                                                                                                             gTTTGGTGCTCGGTGCGGTCGGGTTCAGCTTTACTCAGGGGATGGGTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAg‑g‑ggtc                                                                                                                             <  2:527004/139‑4 (MQ=255)         cTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTAATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTAATATTGCGGCACGCAATAAAACATGATGCATAAAAAAAGACCCTTAG‑G‑G‑‑G‑TCttttttat                                                                                                                  >  6:167884/1‑137 (MQ=255)                  gTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTTCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGc                                                                                                            >  4:461221/1‑139 (MQ=255)                    cGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTc                                                                                                         <  4:409610/139‑1 (MQ=255)                     gggTTCAGCTTTTCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGCAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTc                                                                                                         <  5:332399/139‑1 (MQ=255)                     gggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTc                                                                                                         <  6:208423/139‑1 (MQ=255)                       gTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCa                                                                                                       <  1:413459/139‑1 (MQ=255)                       gTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCa                                                                                                       <  6:177710/139‑1 (MQ=255)                                tACTCAGGTGATGGTTCATGCAGGTGAAACAGGAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCtttt                                                                                                <  7:99154/137‑1 (MQ=255)                                tACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTa                                                                                               >  6:317162/1‑138 (MQ=255)                                tACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTAt                                                                                              >  7:331765/1‑139 (MQ=255)                                 aCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATc                                                                                             >  4:101786/1‑139 (MQ=255)                                 aCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTATTCAGCTTCCATTGATTTa                                                                                               >  2:500033/1‑137 (MQ=255)                                     aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                           <  4:349745/137‑1 (MQ=255)                                     aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                           <  2:69534/137‑1 (MQ=255)                                     aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCa                                                                                         <  6:370184/139‑1 (MQ=255)                                     aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGAAGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                           <  2:483040/137‑1 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCa                                                                                                       <  1:519614/123‑1 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCa                                                                                                       >  2:519614/1‑123 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCAtt                                                                                                     <  4:517985/125‑1 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCAtt                                                                                                     >  3:517985/1‑125 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                           <  8:343641/135‑1 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                           >  7:343641/1‑135 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAg                                                                                        <  6:460715/138‑1 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAg                                                                                        >  3:26404/1‑138 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                       >  1:408780/1‑139 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                       >  5:337262/1‑139 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                       >  5:455945/1‑139 (MQ=255)                                       gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTATTCAGCTTCCATTCTTTTATGGTCAGc                                                                                       >  5:460715/1‑139 (MQ=255)                                          aTGGTTCATGCAGGTGAATCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                     <  7:352893/138‑1 (MQ=255)                                          aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGAGGCACGCAATCAAACATGAAGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTATATTATAGTCAGCt                                                                                      >  5:483367/1‑137 (MQ=255)                                              ttCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCagaag                                                                                <  4:395459/139‑1 (MQ=255)                                                 aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                           <  4:209928/125‑1 (MQ=255)                                                 aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                           >  3:209928/1‑125 (MQ=255)                                                       gTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                             >  7:475386/1‑133 (MQ=255)                                                       gTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc                                                                             <  8:475386/133‑1 (MQ=255)                                                         gAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGcc                                                                     <  3:183634/139‑1 (MQ=255)                                                                       aGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCg                                                        >  4:469651/1‑138 (MQ=255)                                                                           gggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATgg                                                   >  7:57750/1‑139 (MQ=255)                                                                                     ttCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGC‑TCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                         >  4:88822/1‑138 (MQ=255)                                                                                      tCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                         <  2:408780/138‑1 (MQ=255)                                                                                      tCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                         <  4:26404/138‑1 (MQ=255)                                                                                      tCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGc                                        >  8:125551/1‑139 (MQ=255)                                                                                       cATATTGCGGCACGAAAT‑AAAAATTAGTCATACAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTATATTATCGTCATCTCAGAAGATCCACTTGCCACCCGCGGATCCGCATGGCCGATTTCCGCt                                       <  5:44288/138‑1 (MQ=255)                                                                                        atatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGTG‑GTAT‑TTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGATCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                       <  6:483367/138‑1 (MQ=255)                                                                                         ttttGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGc                                     <  1:500033/137‑1 (MQ=255)                                                                                         tatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGc                                     <  3:101786/139‑1 (MQ=255)                                                                                           ttGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                       >  5:103122/1‑135 (MQ=255)                                                                                           ttGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                       <  6:103122/135‑1 (MQ=255)                                                                                            tGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAACGGATCCGCATGGCCGATTTCCGCTGCctc                                  >  2:29354/1‑139 (MQ=255)                                                                                              cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCa                                                                           <  7:164246/96‑1 (MQ=255)                                                                                              cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCa                                                                           >  8:164246/1‑96 (MQ=255)                                                                                              cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTGCGCTGCgtcgt                                >  3:86625/1‑137 (MQ=255)                                                                                                  aCGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAg                            <  8:57750/139‑1 (MQ=255)                                                                                                   cGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGt                           >  8:7216/1‑139 (MQ=255)                                                                                                       aTCAAAGATGATGCATAAAAACAGACCCTTAG‑GTGTAT‑TTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAAt                       <  3:337205/139‑1 (MQ=255)                                                                                                               tgatgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg               <  6:243626/139‑1 (MQ=255)                                                                                                               tgatgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg               <  3:420341/139‑1 (MQ=255)                                                                                                               tgatgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg               <  2:404420/139‑1 (MQ=255)                                                                                                               tgatgCATAAAAAAAGAAGCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTCTCGTCAGCTCAGAAGATCCACTTGCCACCAGCTGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg               <  2:520184/139‑1 (MQ=255)                                                                                                                gatgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCCTGGCCGATTTCCGCTGCCTCTGCCAGTGAATCTGCTTCGa              >  7:306623/1‑139 (MQ=255)                                                                                                                gatgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGc                                     <  8:41913/116‑1 (MQ=255)                                                                                                                gatgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGc                                     >  7:41913/1‑116 (MQ=255)                                                                                                                gatgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGa              >  8:307887/1‑139 (MQ=255)                                                                                                                 atgCATAAAAACAGTCCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCATATCAGCAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTGGAt             <  1:516913/139‑1 (MQ=255)                                                                                                                 atgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt             <  1:175079/139‑1 (MQ=255)                                                                                                                 atgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGATTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGa              <  5:317162/138‑1 (MQ=255)                                                                                                                  tgCATAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt             >  2:175079/1‑138 (MQ=255)                                                                                                                     aTAAAAAAAGACCCTTCG‑G‑GGTCTTTTTTATTGCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACa         <  4:237723/139‑1 (MQ=255)                                                                                                                     aTAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACa         <  2:69201/139‑1 (MQ=255)                                                                                                                      tAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCCCCAGCGGGTCCGCATGGCCGATTTCCGCTGCCTCGTGCAGGGAATCGGCTTCGATGACa         >  2:180686/1‑138 (MQ=255)                                                                                                                      tAAAAAAAGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGATGCCTCTTCCAGTGAATCTGCTTCGATGACa         >  2:21854/1‑138 (MQ=255)                                                                                                                       aaaaaaaGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATa       >  2:401041/1‑139 (MQ=255)                                                                                                                          aaaaGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACg     >  4:480360/1‑138 (MQ=255)                                                                                                                           aaaGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGC‑TCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTc  <  3:88822/139‑1 (MQ=255)                                                                                                                            aaGACCCTTAG‑G‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTc  <  1:18096/139‑1 (MQ=255)                                                                                                                                           ||                                                                                                                             GTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑G‑G‑‑C‑TTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTC  >  BS168_reference_genome/1317017‑1317275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑