Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,037	+T	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,037	1	.	T	93.5%	275.8 / 10.8	93	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base . (5/1);  new base T (24/63);  total (29/64)
Fisher's exact test for biased strand distribution p-value = 1.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑A‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAG  >  BS168_reference_genome/165900‑166162                                                                                                                                               |                                                                                                                              gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑at                                                                                                                               <  2:77530/139‑2 (MQ=255) gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑at                                                                                                                               <  2:281458/139‑2 (MQ=255)    tAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTAGAATCCCGTCTTACGCG‑CCA‑A‑TT‑atgc                                                                                                                             >  2:480625/1‑135 (MQ=21)      aaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCTATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTaa                                                                                                                          <  8:68804/139‑1 (MQ=255)          tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACggg                                                                                                                      <  1:306271/139‑1 (MQ=255)          tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACggg                                                                                                                      <  7:304522/139‑1 (MQ=255)          tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACggg                                                                                                                      <  1:170333/139‑1 (MQ=255)            ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGCTTCCCGTCTTCCCCT‑CCA‑TATT‑T‑CCGAACGGGat                                                                                                                    >  6:46279/1‑139 (MQ=21)            ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGat                                                                                                                    <  6:25598/139‑1 (MQ=255)            ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGGGTCCGCG‑CCATT‑TT‑A‑CCTAACGGGat                                                                                                                    >  3:17434/1‑139 (MQ=25)            ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTGGAATCCCGGCGTCCGCG‑CCC‑T‑TT‑ATCCGAACggggt                                                                                                                    >  2:177306/1‑137 (MQ=21)             tattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGata                                                                                                                   >  3:370510/1‑139 (MQ=255)               ttaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATAtt                                                                                                                 <  6:306951/139‑1 (MQ=255)                  ccAAGGTTTCTCATAAGGATCAAGCTTTTTTTATTGCTATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGt                                                                                                               <  8:521883/138‑1 (MQ=21)                  ccAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGt                                                                                                               >  5:174950/1‑138 (MQ=255)                    aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTaaa                                                                                                            >  1:308913/1‑139 (MQ=255)                     aGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAaat                                                                                                           <  7:388652/139‑1 (MQ=255)                              aTAAGGAGAATGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAaattgaattg                                                                                                    <  2:139015/137‑1 (MQ=255)                              aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCTAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAaattgaattg                                                                                                    <  2:181509/137‑1 (MQ=255)                              aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAaattgaattg                                                                                                    <  8:236145/137‑1 (MQ=255)                              aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGCTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTGCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGTATATTGTAAATTGAATTgc                                                                                                   <  6:499476/138‑1 (MQ=21)                                 aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGt                                                                                                               >  7:382405/1‑123 (MQ=255)                                 aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGt                                                                                                               <  8:382405/123‑1 (MQ=255)                                 aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGccc                                                                                               >  8:369403/1‑139 (MQ=255)                                  ggAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGAGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                              <  7:369403/139‑1 (MQ=255)                                     tgaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                           <  7:287918/137‑1 (MQ=255)                                     gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                           <  4:370510/139‑1 (MQ=255)                                     gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                           <  7:169955/139‑1 (MQ=255)                                     gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                           <  3:143237/139‑1 (MQ=255)                                     gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                           <  5:275460/139‑1 (MQ=255)                                     agaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                           <  1:239131/137‑1 (MQ=255)                                      aaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCAAAGGTGGGGGTCGCGGGGTCGAAAGCCCGCTTACCCTCCCA‑T‑TA‑T‑ACTAACGGGTAATTGTAGATTGAATTTCGCCCCTacc                                                                                          >  5:197742/1‑137 (MQ=12)                                       aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                         <  6:174950/139‑1 (MQ=255)                                       aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                         <  5:499186/139‑1 (MQ=255)                                       aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                         <  4:437449/139‑1 (MQ=255)                                         gCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCa                                                                                       <  6:33387/139‑1 (MQ=255)                                          cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                      <  3:396438/139‑1 (MQ=255)                                          cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                      <  2:518492/139‑1 (MQ=255)                                          cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                      <  6:248974/139‑1 (MQ=255)                                          cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                      <  7:86463/139‑1 (MQ=255)                                          cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                      <  3:18297/139‑1 (MQ=255)                                             tttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCCCCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATGCTAACGGGATATTGTATATTGAATTTCGCCCGTAGCTCAAttt                                                                                   >  5:96794/1‑138 (MQ=21)                                             tttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGGAAATTGAATTTCGCCCGTAGCTGAATTg                                                                                   >  4:174101/1‑139 (MQ=21)                                             tttttATTGCGATATGCGGAAGGAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAt                                                                                     <  5:97991/137‑1 (MQ=255)                                                  aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCTCCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                               <  1:145297/138‑1 (MQ=255)                                                  aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                               <  1:480625/138‑1 (MQ=255)                                                  aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                               <  2:308913/138‑1 (MQ=255)                                                  aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                               <  3:38345/138‑1 (MQ=255)                                                  aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                               <  1:48381/138‑1 (MQ=255)                                                  aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                               <  5:46279/138‑1 (MQ=255)                                                  aTTGCGATATGCGGAAGTAGGTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                               <  5:319187/138‑1 (MQ=255)                                                   ttGCGATATGCGGAAGTAGATCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGTTaga                                                                             <  8:185634/139‑1 (MQ=255)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGa                                                                                                                     <  5:118/98‑1 (MQ=255)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGa                                                                                                                     >  6:118/1‑98 (MQ=255)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCGCAATTGGATaga                                                                             <  2:166916/138‑1 (MQ=255)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCG‑CCA‑A‑TT‑ATCCTAACGGGATATTTTAAATTGAATTGCGCCCGTAGCTCAATTGGATagct                                                                            >  3:144557/1‑137 (MQ=21)                                                     gCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGTGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATagag                                                                            <  1:130751/138‑1 (MQ=255)                                                         tatGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGttt                                                                       <  4:458094/139‑1 (MQ=255)                                                              ggAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTa                                                                  <  5:390672/139‑1 (MQ=255)                                                                 agtagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTAGAGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACg                                                                <  4:30732/138‑1 (MQ=255)                                                                     gtTCAGTGGTAGAACACCACCTTGCCCAGGTGGGGGTCGCGGGTGCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTACCGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGCTAGAGCGTTTGACTACGGATCa                                                           <  6:309425/139‑1 (MQ=25)                                                                     gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATc                                                            >  5:489173/1‑138 (MQ=255)                                                                     gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCa                                                           <  4:502880/139‑1 (MQ=255)                                                                     gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCa                                                           <  5:389531/139‑1 (MQ=255)                                                                      ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGt                                                                                                               >  1:167313/1‑86 (MQ=255)                                                                      ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGt                                                                                                               <  2:167313/86‑1 (MQ=255)                                                                      ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                          <  7:465343/139‑1 (MQ=255)                                                                      ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                          <  7:462024/139‑1 (MQ=255)                                                                           tGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATGCCGTCTTGCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCGCAATTGGTTATAGCGTTTGAATAAGGATCAAAAGGt                                                     >  6:374467/1‑139 (MQ=25)                                                                             gTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGtt                                                    <  3:478726/138‑1 (MQ=255)                                                                             gTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGtt                                                    <  1:17055/138‑1 (MQ=255)                                                                             gTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTACGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGt                                                     >  3:472987/1‑137 (MQ=255)                                                                              tAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGTATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAg                                                  <  8:219976/139‑1 (MQ=255)                                                                                     ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAaattgaattg                                                                                                    <  4:525418/82‑1 (MQ=255)                                                                                     ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAaattgaattg                                                                                                    >  3:525418/1‑82 (MQ=255)                                                                                     ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTTCGGATCAAAAGGTTAGGGGTTCg                                           >  7:344522/1‑139 (MQ=255)                                                                                      caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                          >  6:250195/1‑139 (MQ=255)                                                                                      caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                          <  4:155821/139‑1 (MQ=255)                                                                                      caccTTGCCAAGGTGGGGGTCGCGGGTGCGAAGCCCGTCTTCCGCT‑CCA‑T‑TTCT‑CCTCACGGGATATTGTAAAGTGAATTGCGCCCGGAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGTGTTCGa                                          <  6:296976/139‑1 (MQ=25)                                                                                        ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTGTTGCGCG‑CCATT‑TT‑T‑CCTAACGGGATATTTTAAATTGAATTGGGCCCGTAGCTCAATTGGATTTAGCGTTTGACTACGGATCAATAGGTTAGGGGttgggct                                        >  2:438202/1‑134 (MQ=25)                                                                                        ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                        <  2:75785/139‑1 (MQ=255)                                                                                        ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCGAACGGGATATTGTAAATTGAATTGCGCCCGTTGCTCAAGTGGATTGAGCGTTTGCCTACGGATCAAAAGGTTAGGGGTTCGAct                                        >  5:234869/1‑139 (MQ=37)                                                                                        ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATACTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                        >  7:438906/1‑139 (MQ=255)                                                                                        ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATACCGTCTTACGCT‑CCA‑T‑TT‑ATACTAACGGGATATTGTTAATTTAATTGCGCCCGTAGCGCAAATGGATATAGCGTTTGACTACGGATCAAAAGGGTAGGGGTTAGaat                                        >  8:373585/1‑137 (MQ=25)                                                                                           tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCATT‑TT‑T‑CCAAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcct                                     >  8:39346/1‑139 (MQ=255)                                                                                           tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcct                                     >  7:472539/1‑139 (MQ=255)                                                                                                aGGTGGGGGTCGCGGGTTCGAATCCCGTCTTACGCT‑CCA‑T‑TT‑ATACTAACGGGATATTGTTAATTTAATTTCGCCCGTATCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCATCTAgg                                >  3:225226/1‑139 (MQ=21)                                                                                                     ggggTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcgcg                           <  6:71494/139‑1 (MQ=255)                                                                                                        gTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCa                        <  8:17150/139‑1 (MQ=255)                                                                                                        gTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCa                        <  3:384644/139‑1 (MQ=255)                                                                                                        gTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCa                        <  8:472539/139‑1 (MQ=255)                                                                                                                  cGATTCCCGGCTTCCGCT‑CCA‑T‑TT‑ATGCTAACGGGATTTTGTAGATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaa              <  6:197742/139‑1 (MQ=17)                                                                                                                   gAATCCCGTCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAATAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaa              <  3:423712/138‑1 (MQ=255)                                                                                                                    aaTCCCGTCTTCCTCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaata            <  4:17434/139‑1 (MQ=255)                                                                                                                           tCTTCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaa         >  8:495162/1‑135 (MQ=255)                                                                                                                              tCCGCT‑CCA‑T‑TT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatagatag  >  1:311899/1‑139 (MQ=255)                                                                                                                                               |                                                                                                                              GCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCT‑CCA‑T‑TT‑A‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAG  >  BS168_reference_genome/165900‑166162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑