Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,589,333	G→A	A145T (GCC→ACC)	murD →	UDP‑N‑acetylmuramoylalanyl‑D‑glutamate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,589,333	0	G	A	100.0%	219.2 / NA	64	A145T (GCC→ACC)	murD	UDP‑N‑acetylmuramoylalanyl‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/28);  total (36/28)

TTTGGACGGAAATTGAGCTCGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTGCCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGACCGGAAATCAGCCT  >  BS168_reference_genome/1589199‑1589457                                                                                                                                       |                                                                                                                             tttGGACGGAAATTGAGCTCGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCg                                                                                                                           >  7:510551/1‑138 (MQ=255) tttGGACGGAAATTGAGCTCGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCg                                                                                                                           >  5:157741/1‑138 (MQ=255)   tgtaCGTAATTTGAGCTCGCTTACTACCTTACCAGCGCCAATTTTTTGGGTTTTACGGGATCGAACGTAAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGa                                                                                                                         <  6:522800/135‑1 (MQ=255)   tGGACGGAAATTGAGCTCGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGa                                                                                                                         >  3:263745/1‑138 (MQ=255)     gACGGAAATTGAGCTCGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGT‑AAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACa                                                                                                                     >  4:524438/1‑139 (MQ=255)       cGGAAATTGAGCTCGCTTACTACCTTACCAGCGCCAAGTTTATGGGGATAACCGGATCTAACGGAAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACAt                                                                                                                    <  6:152525/139‑1 (MQ=255)                aGCTCGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGt                                                                                                           <  8:510551/139‑1 (MQ=255)                  cTCGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGCCAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCg                                                                                                         >  4:232474/1‑139 (MQ=255)                    cGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGc                                                                                                                <  5:218545/130‑1 (MQ=255)                    cGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGc                                                                                                                >  6:218545/1‑130 (MQ=255)                    cGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGc                                                                                                        >  4:357161/1‑138 (MQ=255)                            acCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGCCCACGACAACGCTAATTTACGAAATGCTCAAGGCTGCCTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGt                                                                                               >  8:493548/1‑139 (MQ=255)                                gACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGcc                                                                                           >  3:485421/1‑139 (MQ=255)                                 aCCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGCCCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGcc                                                                                           >  2:180016/1‑138 (MQ=255)                                 aCCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAATACGCACTGATTACCGGAAACATCGCCACAGTCGCAAGTGAGGTTGcc                                                                                           >  2:497580/1‑138 (MQ=255)                                 aCCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGcc                                                                                           >  5:39348/1‑138 (MQ=255)                                 aCCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGcc                                                                                           >  7:62763/1‑138 (MQ=255)                                 aCCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGcc                                                                                           >  3:455784/1‑138 (MQ=255)                                 aCCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGcc                                                                                           >  3:327836/1‑138 (MQ=255)                                     gcgcCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATc                                                                                       >  8:370476/1‑138 (MQ=255)                                     gcgcCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCa                                                                                      >  8:452194/1‑139 (MQ=255)                                     gcgcCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCa                                                                                      <  6:150457/139‑1 (MQ=255)                                     gcgcCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCa                                                                                      >  4:273657/1‑139 (MQ=255)                                     gcgaCAAGTTTATCGGGATTACCGGATCGAACGGAAAAACTACAACAACAACACTAATTAACGAAAAGCTCAACGCAGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTACAAAGATAGTTTGCCTATCa                                                                                      >  6:166060/1‑139 (MQ=255)                                       gccattttttTCGGTATTCCGGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCGCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATg                                                                                    <  5:166060/133‑1 (MQ=255)                                                       ttACCGGATCGAACGGTAAAACGACCACGCCAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATg                                                                    >  7:132685/1‑139 (MQ=255)                                                       ttACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATg                                                                    >  4:121375/1‑139 (MQ=255)                                                         aCCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGa                                                                                                                         >  6:168342/1‑84 (MQ=255)                                                         aCCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGa                                                                                                                         <  5:168342/84‑1 (MQ=255)                                                          ccGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGAt                                                                 <  3:341780/139‑1 (MQ=255)                                                          ccGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGAt                                                                 <  1:175772/139‑1 (MQ=255)                                                            ggAACGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCg                                                               <  1:57258/139‑1 (MQ=255)                                                               tCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTa                                                            >  2:159869/1‑139 (MQ=255)                                                               tCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTa                                                            >  3:25350/1‑139 (MQ=255)                                                               tCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACAGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTa                                                            >  2:405303/1‑139 (MQ=255)                                                                    cGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAAGCTCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGaa                                                       <  4:327836/139‑1 (MQ=255)                                                                    cGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGaa                                                       <  7:370476/139‑1 (MQ=255)                                                                    cGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGaa                                                       <  1:159869/139‑1 (MQ=255)                                                                      gTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACt                                                     <  6:157741/139‑1 (MQ=255)                                                                         aaaCGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTc                                                  <  3:357161/139‑1 (MQ=255)                                                                               ccACGCCAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGtt                                            >  1:353208/1‑139 (MQ=255)                                                                               ccACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCgat                                            >  6:125378/1‑137 (MQ=255)                                                                               ccACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGtt                                            >  4:435841/1‑139 (MQ=255)                                                                                cACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGttt                                           <  3:273657/139‑1 (MQ=255)                                                                                               ttACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAaca                            >  6:493243/1‑139 (MQ=255)                                                                                                  cGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAacaca                          <  1:180016/138‑1 (MQ=255)                                                                                                  cGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAacaca                          <  4:25350/138‑1 (MQ=255)                                                                                                       tGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATg                                                                              >  8:135029/1‑81 (MQ=255)                                                                                                       tGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATg                                                                              <  7:135029/81‑1 (MQ=255)                                                                                                       tGCTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCttt                    >  8:128360/1‑139 (MQ=255)                                                                                                         cTCAAGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCttt                    >  6:290856/1‑137 (MQ=255)                                                                                                            aaGGCTGACTCGCAAAAAGCACTTATTACCGGAAACTTCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAAAACACGCTTTCAGAc               <  5:357881/139‑1 (MQ=255)                                                                                                            aaGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGAc               >  8:323039/1‑139 (MQ=255)                                                                                                             aGGCTGACTCGCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGAcc              <  5:290856/139‑1 (MQ=255)                                                                                                                   aCTCGCAAAAAGCACTGATTACCGGAAACATCGGCAAAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGACCACACGCTTTCAGCCCGGaaag        >  2:397036/1‑138 (MQ=255)                                                                                                                       gCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGACCGGAAATCAGc    <  7:452194/139‑1 (MQ=255)                                                                                                                       gCAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGACCGGAAATCAGc    <  5:419635/139‑1 (MQ=255)                                                                                                                        cAAAAAGCACTGATTACCGGAAACATCGGCAGAGTCGCAAGTGAGGTTGCCTTTCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGACCGGAAATCAGcc   <  4:121262/139‑1 (MQ=255)                                                                                                                        cAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAg                                                                                                 >  6:81568/1‑45 (MQ=255)                                                                                                                        cAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAg                                                                                                 <  5:81568/45‑1 (MQ=255)                                                                                                                        cAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACt                                                     >  5:115868/1‑89 (MQ=255)                                                                                                                        cAAAAAGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACt                                                     <  6:115868/89‑1 (MQ=255)                                                                                                                         aaaaaGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGACCGGAAATCAGCCt  <  7:493548/139‑1 (MQ=255)                                                                                                                         aaaaaGCACTGATTACCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGACCGGAAATCAGCCt  <  2:353208/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                             TTTGGACGGAAATTGAGCTCGCTTACTACCTGACCAGCGCCAAGTTTATCGGGATTACCGGATCGAACGGTAAAACGACCACGACAACGCTAATTTACGAAATGCTCAAGGCTGACTCGCAAAAAGCACTGATTGCCGGAAACATCGGCACAGTCGCAAGTGAGGTTGCCTATCATGCTGATGGAGATGAATGGATCGTTACCGAACTGTCTTCGTTTCAGCTGATGGGAACACACGCTTTCAGACCGGAAATCAGCCT  >  BS168_reference_genome/1589199‑1589457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑