BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,271,424	T→C	R78R (AGA→AGG)	uvrX ←	lesion bypass phage DNA polymerase; phage SPbeta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,271,424	0	T	C	100.0%	341.9 / NA	110	R78R (AGA→AGG)	uvrX	lesion bypass phage DNA polymerase; phage SPbeta
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (41/69); total (41/69)

Only 100 of 110 total aligned reads displayed.

TTTCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAA  >  BS168_reference_genome/2271290‑2271559

tttCCTGGATCTAAAATAGATTCATCAATTGAATACGTATGGACAGATTTCTCAGGAACATATCTGTAAAAGAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCtt                                                                                                                                      <  8:266644/138‑1 (MQ=14)

 ttCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCtt                                                                                                                                      <  1:377550/137‑1 (MQ=255)

    cTGGATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGAt                                                                                                                                  <  4:280972/138‑1 (MQ=255)

    cTGCATCTAATAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGAt                                                                                                                                  <  1:270467/138‑1 (MQ=255)

       cATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAAAAGCTTTGTAATTTCAGTTGAAACTATGATGGAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCtt                                                                                                                               >  1:132691/1‑138 (MQ=255)

           tAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGt                                                                                                                          <  3:27902/139‑1 (MQ=255)

           tAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGt                                                                                                                          >  5:542990/1‑139 (MQ=255)

            aaaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTa                                                                                                                         <  5:387444/139‑1 (MQ=255)

             aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg                                                                                                                            <  7:290093/135‑1 (MQ=255)

             aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGt                                                                                                                          <  4:79817/137‑1 (MQ=255)

             aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTa                                                                                                                         <  1:107763/138‑1 (MQ=255)

             aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTa                                                                                                                         <  3:348845/138‑1 (MQ=255)

                   aTTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCaaa                                                                                                                  <  1:243853/139‑1 (MQ=255)

                   aTTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCaaa                                                                                                                  <  3:43513/139‑1 (MQ=255)

                    ttCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAAt                                                                                                                 <  8:251974/139‑1 (MQ=255)

                          aaTTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGtct                                                                                                            <  2:507237/138‑1 (MQ=255)

                               aaTACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATcc                                                                                                      >  4:91549/1‑139 (MQ=255)

                                 tACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTg                                                                                                    >  1:317836/1‑139 (MQ=255)

                                 tACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTg                                                                                                    >  3:429962/1‑139 (MQ=255)

                                 tACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTg                                                                                                    >  8:376971/1‑139 (MQ=255)

                                      aTGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGtttt                                                                                                >  5:383690/1‑138 (MQ=38)

                                      aTGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGtttt                                                                                                >  6:264169/1‑138 (MQ=38)

                                       tGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTg                                                                                               <  3:397503/138‑1 (MQ=37)

                                       tGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTg                                                                                               <  3:174024/138‑1 (MQ=37)

                                         gACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGAtt                                                                                            >  1:125244/1‑139 (MQ=37)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATc                                                                                                       >  8:414344/1‑123 (MQ=38)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATc                                                                                                       <  7:414344/123‑1 (MQ=38)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                        >  2:33950/1‑138 (MQ=37)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                        >  7:6417/1‑138 (MQ=37)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                        >  2:234292/1‑138 (MQ=37)

                                               tttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAGTGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaaaa                                                                                      >  8:394996/1‑139 (MQ=255)

                                               tttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaaac                                                                                      >  6:467439/1‑138 (MQ=37)

                                                ttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAAt                                                                                     >  2:48970/1‑139 (MQ=37)

                                                ttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAAt                                                                                     >  5:508844/1‑139 (MQ=37)

                                                       gAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                              <  7:327306/139‑1 (MQ=21)

                                                       gAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                              <  6:383690/139‑1 (MQ=21)

                                                       gAACAAATCTGTAAAACAGATTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                              <  7:123681/139‑1 (MQ=21)

                                                       gAAAAAATCTTTAAAACAGCTTTGTTATTTCAGTTGAAACTCTTATGAAAATATTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTAAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                              <  6:287315/139‑1 (MQ=11)

                                                          cAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCATATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAg                                                                           >  4:322604/1‑139 (MQ=21)

                                                          cAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAg                                                                           <  6:21503/139‑1 (MQ=21)

                                                          cAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAg                                                                           >  7:537003/1‑139 (MQ=21)

                                                                  tAAAACAGCTTTGTTATTTCAGGTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAgg                                                                     <  4:12940/137‑1 (MQ=255)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCaaa                                                                                                                  <  7:8162/92‑1 (MQ=37)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                      <  8:257019/136‑1 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                      <  8:159058/136‑1 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                      >  7:257019/1‑136 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                      >  7:159058/1‑136 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                      >  3:417338/1‑136 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTAGCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGAAg                                                                      >  6:487784/1‑136 (MQ=255)

                                                                    aaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCTTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                 <  7:131379/139‑1 (MQ=21)

                                                                    aaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                 <  4:406411/139‑1 (MQ=21)

                                                                         gcgatGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGAGCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                            <  1:201206/135‑1 (MQ=255)

                                                                         gCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                            <  5:176664/139‑1 (MQ=21)

                                                                         gCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGGCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                            <  6:542990/139‑1 (MQ=21)

                                                                         gCTATGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                            <  1:340747/139‑1 (MQ=21)

                                                                          cTTTGTAATTTCAGTCGAAACTCTGATGAAAAGCTTGATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                 <  1:419135/133‑1 (MQ=255)

                                                                          cTTTGTAATTTCAGTCGAAACTCTGATGAAAAGCTTCATATGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                 >  2:419135/1‑133 (MQ=255)

                                                                            ttGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                 >  8:44779/1‑131 (MQ=21)

                                                                             tGTTATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACTTTGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTATTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaa                                                         <  1:520493/138‑1 (MQ=11)

                                                                             tGTAATTTCAGTTGAAACTCTGATGGAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATTGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaa                                                         <  2:132691/138‑1 (MQ=255)

                                                                               tAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaata                                                       <  2:125244/138‑3 (MQ=21)

                                                                                    tCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTg                                                                                                                                     <  5:530045/55‑1 (MQ=255)

                                                                                    tCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAatactacac                                                 >  6:131737/1‑139 (MQ=17)

                                                                                    tCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAatactaca                                                  <  4:215502/138‑1 (MQ=17)

                                                                                    tCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAatactaca                                                  <  2:317836/138‑1 (MQ=17)

                                                                                      acttGAAAATATTATGAAAAGATTCATTTGTTTATTTACAATGTGTATCCTTGTATCTTCAGGTATCACAAATAGTCTCGATCCTTTTTTGATTACAAAATCTTTTTTAATTGCAGGAGATGCAGTTAATACTACACtt                                               <  8:230854/137‑1 (MQ=11)

                                                                                             aCTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTc                                        <  4:417338/139‑1 (MQ=17)

                                                                                             aCTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTc                                        >  1:331106/1‑139 (MQ=17)

                                                                                             aCTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTc                                        <  1:48970/139‑1 (MQ=17)

                                                                                                  gatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                 >  7:513334/1‑109 (MQ=17)

                                                                                                  gatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                 <  8:513334/109‑1 (MQ=17)

                                                                                                  gatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaa                                                         >  4:423176/1‑117 (MQ=17)

                                                                                                  gatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaa                                                         <  3:423176/117‑1 (MQ=17)

                                                                                                   atgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCAAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCtct                                     <  4:314685/136‑3 (MQ=255)

                                                                                                      aaaaGCTTCTTTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                                <  1:203771/138‑1 (MQ=255)

                                                                                                      aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                                <  2:55316/138‑1 (MQ=12)

                                                                                                      aaaaGCTTCATTTGTGGATTTAAAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACAACACTTCCCTGTCTCTCCGTAt                               <  5:76285/139‑1 (MQ=11)

                                                                                                           cTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATg                                                                <  2:272886/101‑1 (MQ=17)

                                                                                                              cATTTGTGGATTTACAATGTGTATCCTTGGATCTTCATGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                              <  8:307443/84‑1 (MQ=255)

                                                                                                              cATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                              >  7:307443/1‑84 (MQ=18)

                                                                                                              cATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGAGCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac                        <  6:508844/138‑1 (MQ=255)

                                                                                                              cATTTGGGGATTTACTATGTGTACCCTTGGAGCTTCTGGTAGCTCAAATAGTCTCGATCCGGTTTTGATTCCAAAATCTTTTTTCAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac                        <  1:212852/138‑1 (MQ=11)

                                                                                                                   gtgGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTATTACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAg                   <  7:376971/138‑1 (MQ=255)

                                                                                                                   gtgGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAg                   <  4:189672/138‑1 (MQ=255)

                                                                                                                   gtgGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGGCTCGATCGTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAg                   <  8:526187/138‑1 (MQ=11)

                                                                                                                      gATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCCGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                                <  2:245733/122‑1 (MQ=255)

                                                                                                                      gATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                                >  1:245733/1‑122 (MQ=12)

                                                                                                                            cAATGTGTATCCTTGGATCTTCTGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc          <  1:33950/138‑1 (MQ=255)

                                                                                                                            cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc          <  8:537003/138‑1 (MQ=255)

                                                                                                                            cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc          <  4:364524/138‑1 (MQ=255)

                                                                                                                            cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc          <  1:234292/138‑1 (MQ=255)

                                                                                                                                gtgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGt      >  3:249507/1‑138 (MQ=255)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCTATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGtt     >  1:420737/1‑138 (MQ=255)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTacaa                      >  5:440033/1‑121 (MQ=9)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTacaa                      <  6:440033/121‑1 (MQ=9)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAg                   <  8:61517/124‑1 (MQ=255)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAg                   >  7:61517/1‑124 (MQ=255)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa    <  8:6417/139‑1 (MQ=255)

                                                                                                                                   tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaaa  <  6:246147/139‑1 (MQ=255)

                                                                                                                                   tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa   <  1:329373/138‑1 (MQ=255)

TTTCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAA  >  BS168_reference_genome/2271290‑2271559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`