BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	165,751	Δ1 bp	intergenic (+44/‑3)	rrnI‑5S → / → trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,751	0	C	.	100.0%	445.9 / NA	124	intergenic (+44/‑3)	rrnI‑5S/trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (57/67); total (57/67)

Only 100 of 124 total aligned reads displayed.

GTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAA  >  BS168_reference_genome/165621‑165871

gTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                  >  2:356945/1‑138 (MQ=255)

gTTCCCATACCGAACACGGAAGTTAAGCTCTACAGCGCCGATGGAAGTCGGGGGTTT‑CCCCCAGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGATTGTATATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                  >  2:216592/1‑138 (MQ=18)

 ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                  >  1:185334/1‑137 (MQ=255)

 ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                  >  6:173662/1‑137 (MQ=255)

 ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                  >  7:18536/1‑137 (MQ=255)

  tCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                               >  8:252845/1‑139 (MQ=255)

  tCCCATACCGAACACGGAAGGTAAGCGCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                               <  4:65669/139‑1 (MQ=21)

   cccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                               <  8:196269/138‑1 (MQ=255)

    ccATACCGACCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                             <  1:179988/139‑1 (MQ=255)

    ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                             <  7:252845/139‑1 (MQ=255)

    ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                             <  6:203990/139‑1 (MQ=255)

    ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                             <  6:32479/139‑1 (MQ=255)

    ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGGCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                             <  5:31246/139‑1 (MQ=255)

    ccATACCGAAAACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCGCTGTGAGTGTAGGACGCCGACAAGCAGATTTATTTATTCTACCATGTTTGTTTCTGAAGCTTTATAT‑T‑ATTCCGCAGTTGCt                                                                                                             <  4:250794/139‑1 (MQ=18)

     cATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTc                                                                                                            <  6:292065/139‑1 (MQ=255)

      aTACCGAACACGGAAGTTAATCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGATTTATAT‑T‑ATTCCGCAGTAGcg                                                                                                             >  6:241478/1‑136 (MQ=21)

      aTACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGc                                                                                                              <  5:358326/136‑1 (MQ=255)

          cGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                               <  8:28596/131‑1 (MQ=255)

          cGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                               >  7:28596/1‑131 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATTT‑‑TTCCGCAGTAGCTCAGTgg                                                                                                       >  2:143932/1‑137 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                             >  7:107901/1‑131 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                       >  1:25527/1‑137 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                       >  3:347047/1‑137 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                       >  6:167494/1‑137 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                       >  7:248356/1‑137 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                       >  7:204460/1‑137 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                       >  1:385735/1‑137 (MQ=255)

            aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                     <  3:392402/139‑1 (MQ=255)

             acacGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                     <  1:119605/139‑1 (MQ=25)

              cacGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                     >  8:307967/1‑137 (MQ=255)

               acGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTagag                                                                                                  <  1:356945/139‑1 (MQ=255)

                 ggAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                             >  7:299507/1‑126 (MQ=255)

                 ggAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                                 >  8:136468/1‑138 (MQ=255)

                   aaGTTAAGCTCTTCCGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                              <  1:170108/139‑1 (MQ=255)

                   aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                              <  6:387001/139‑1 (MQ=255)

                   aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGGTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                              <  1:164615/139‑1 (MQ=255)

                   aaGTTAAGCTCTGCAGCGCCGATGGTAGGCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                              <  6:152471/139‑1 (MQ=25)

                   aaGTTAAGCGCTTCAGCGCCGATGGTAGTCGGGGGGTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                              <  4:243659/139‑1 (MQ=25)

                   aaGGTAAGCGCGTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                              <  2:345156/139‑1 (MQ=25)

                        aaGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGc                                                                                          <  4:274829/138‑1 (MQ=255)

                           ctctTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGtt                                                                                      <  4:25569/139‑1 (MQ=255)

                           ctctTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGt                                                                                       >  7:31958/1‑138 (MQ=255)

                                        aTGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                         >  4:31941/1‑139 (MQ=255)

                                        aTGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                         >  2:124284/1‑139 (MQ=255)

                                        aTGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                         >  7:13975/1‑139 (MQ=255)

                                              gTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGt                                                                    <  6:133765/138‑1 (MQ=255)

                                                      ttt‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCt                                                           <  7:38334/139‑1 (MQ=255)

                                                         tgccccTGTGTCAGTAGGTCGCCGCCAAGCAGATTTATTAATTATACCATGTTTGTTTTTTAAGCTTTATAT‑T‑ATTCCGCCGTAGCTCAGGGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTGGAAtactac                                                         >  1:291913/3‑139 (MQ=21)

                                                              cTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCg                                                    <  7:183080/139‑1 (MQ=255)

                                                                gtgAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCg                                                    >  2:342092/1‑137 (MQ=255)

                                                                gtgAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGa                                                  <  2:385735/139‑1 (MQ=255)

                                                                 tgAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAg                                                 >  6:282574/1‑139 (MQ=255)

                                                                 tgAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAg                                                 >  3:325164/1‑139 (MQ=255)

                                                                     agTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                            >  3:195546/1‑136 (MQ=255)

                                                                      gTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                           <  5:173662/139‑5 (MQ=21)

                                                                          gACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCtt                                        >  7:356232/1‑139 (MQ=21)

                                                                          gACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCtt                                        >  5:153416/1‑139 (MQ=21)

                                                                           aCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCt                                                                                                >  7:306264/1‑82 (MQ=38)

                                                                           aCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCt                                                                                                <  8:306264/82‑1 (MQ=38)

                                                                            cgccgcCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTc                                       >  7:174136/1‑138 (MQ=21)

                                                                             gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                     <  1:342092/139‑1 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                     >  7:136919/1‑137 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                     >  7:223704/1‑137 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                     >  7:271548/1‑137 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                     >  4:22091/1‑137 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                     >  5:75925/1‑137 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                     >  5:159408/1‑137 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATa                                   <  8:13975/139‑1 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                           >  3:96396/1‑126 (MQ=18)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                           <  4:96396/130‑5 (MQ=18)

                                                                                    aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑AATCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGGTAACCGATCGGTCGTAGGTTCGAATCCAACCTGCGGAGCCATTGCTTCCATAGCTca                              <  2:227457/139‑1 (MQ=21)

                                                                                      cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                            <  5:282574/139‑1 (MQ=21)

                                                                                      cAGATTTATTAATGCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                            <  1:214300/139‑1 (MQ=18)

                                                                                         aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCTGCAgg                         >  8:305413/1‑139 (MQ=18)

                                                                                         aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                         <  7:56316/139‑1 (MQ=21)

                                                                                         aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                         <  3:22091/139‑1 (MQ=21)

                                                                                         aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                         >  2:132802/1‑139 (MQ=21)

                                                                                          tttATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                         <  6:153416/138‑1 (MQ=21)

                                                                                          tttATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGATCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                         <  8:248356/138‑1 (MQ=21)

                                                                                              ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                    <  4:347047/139‑1 (MQ=21)

                                                                                                 aTTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCa                  <  8:18536/138‑1 (MQ=21)

                                                                                                   tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTca                              <  5:207418/124‑1 (MQ=18)

                                                                                                   tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTca                              >  6:207418/1‑124 (MQ=18)

                                                                                                   tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt               >  8:250234/1‑139 (MQ=21)

                                                                                                   tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACt                >  3:267782/1‑138 (MQ=21)

                                                                                                     tACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTc                                       <  3:366935/113‑1 (MQ=17)

                                                                                                     tACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTc                                       >  2:215891/1‑113 (MQ=17)

                                                                                                     tACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTc                                       <  1:215891/113‑1 (MQ=17)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa            <  7:381017/138‑1 (MQ=21)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa            <  5:167494/138‑1 (MQ=21)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa            <  5:144740/138‑1 (MQ=21)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCGTCCATAGCGCATCAGGTAGAGCACTTCCa            <  5:28253/138‑1 (MQ=18)

                                                                                                            tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                     <  2:70574/108‑1 (MQ=14)

                                                                                                            tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGt        >  1:91825/1‑137 (MQ=21)

                                                                                                            tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa       <  7:250234/138‑1 (MQ=21)

                                                                                                            tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa       <  8:356232/138‑1 (MQ=21)

                                                                                                               gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAgg    >  3:280194/1‑138 (MQ=21)

                                                                                                                tttATGATGCTTTATAT‑T‑ATTCCGCAGTCGCTCAGTGGAAGAGCTATCGGCTGTTAACCGCTCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCGCAGCAGGTAGAGCACTTCCATGGTAAGGaa  <  4:55975/139‑1 (MQ=18)

                                                                                                                    tGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa            >  2:226937/1‑125 (MQ=18)

                                                                                                                    tGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa            <  1:226937/125‑1 (MQ=18)

GTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAA  >  BS168_reference_genome/165621‑165871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`