Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	376,025	(G)8→7	intergenic (‑227/‑7)	hxlA ← / → hxlR	3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	376,018	0	G	.	100.0%	346.1 / NA	76	intergenic (‑220/‑14)	hxlA/hxlR	3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (35/41);  total (35/41)

TTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAGGGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTC  >  BS168_reference_genome/375884‑376151                                                                                                                                       |                                                                                                                                      ttACTTTGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  2:187338/139‑1 (MQ=255) ttACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  7:6036/139‑1 (MQ=255) ttACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  5:316537/139‑1 (MQ=255) ttACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGATTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  1:24836/139‑1 (MQ=255)  tACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  6:125961/138‑1 (MQ=255)    cTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  1:228801/139‑2 (MQ=255)    cTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  4:129852/139‑2 (MQ=255)    cTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  2:127869/139‑2 (MQ=255)    cTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  6:347957/139‑2 (MQ=255)    cTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGGCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  4:318372/139‑2 (MQ=255)    cTTGGAGGATACAGTGAGGAGAGACAATCAGATTTCTTTCGCGTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  7:223446/139‑2 (MQ=255)     ttGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggggat                                                                                                                               >  4:75778/1‑137 (MQ=255)     ttGGAGGATACTGTGAGGAGAGACAATCAGATTTCTAACGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGTTAACTAAgggggggat                                                                                                                               >  1:249060/1‑137 (MQ=255)       ggaggaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggggattc                                                                                                                             >  4:100568/1‑135 (MQ=255)       ggaggaTACTGGGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggggattc                                                                                                                             <  2:284358/139‑5 (MQ=255)         aggaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTa                                                                                                                          <  4:170288/139‑1 (MQ=255)           gaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGGTACTAA‑GGGGGGGATTCTAtt                                                                                                                        <  8:359264/139‑1 (MQ=255)              aCTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAg                                                                                                                     >  4:187642/1‑139 (MQ=255)               cTGTGAGTAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAATATTCTGCAAAAAATTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTAGTAA‑GGGGGGGATTCTATTGAg                                                                                                                     <  2:249060/138‑1 (MQ=255)               cTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAg                                                                                                                     <  3:283972/138‑1 (MQ=255)                  tgAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCg                                                                                                                  >  4:202125/1‑138 (MQ=255)                     ggagAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATg                                                                                                              <  5:177702/139‑1 (MQ=255)                      gagagaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATgg                                                                                                             <  1:38258/139‑1 (MQ=255)                       agagaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGga                                                                                                            >  6:126589/1‑139 (MQ=255)                         agaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgac                                                                                                           >  6:343685/1‑138 (MQ=255)                          gaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgacga                                                                                                         >  2:356797/1‑139 (MQ=255)                           aCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgacga                                                                                                         >  6:35966/1‑138 (MQ=255)                            cAATCAGATTTCTTTCGCTTGATTTTATGAGTGACTAAAAAGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACAAACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACa                                                                                                       <  1:371579/139‑1 (MQ=255)                             actCAGATTGCTTTCGCTTGATATATTGAGAGATTCTCATCAAAAAAGAGTCTCCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGCTGGACGACaa                                                                                                      <  7:334997/137‑1 (MQ=255)                              aTCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  3:324223/139‑1 (MQ=255)                              aTCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  5:343685/139‑1 (MQ=255)                              aTCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  5:126589/139‑1 (MQ=255)                              aTCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  4:68802/139‑1 (MQ=255)                              aTCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  4:291591/139‑1 (MQ=255)                              aTCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  3:202125/139‑1 (MQ=255)                                                gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  8:163576/137‑1 (MQ=255)                                                gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  6:194157/137‑1 (MQ=255)                                                gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  1:356797/137‑1 (MQ=255)                                                gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  7:268777/137‑1 (MQ=255)                                                 aTTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGCATGGACGACAAAAGGTTTAATTGTCAGAAg                                                                                   >  5:85507/1‑138 (MQ=255)                                                           tgagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCt                                                                        >  3:135818/1‑139 (MQ=255)                                                           tgagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCt                                                                        >  2:389700/1‑139 (MQ=255)                                                           tgagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCt                                                                        <  3:75778/139‑1 (MQ=255)                                                            gagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCtt                                                                       >  4:209930/1‑139 (MQ=255)                                                            gagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCtt                                                                       >  4:214999/1‑139 (MQ=255)                                                             agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  5:42388/108‑1 (MQ=255)                                                             agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     >  6:42388/1‑108 (MQ=255)                                                             agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  5:289664/124‑1 (MQ=255)                                                             agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     >  6:289664/1‑124 (MQ=255)                                                             actataATGAAAAAAGAGTATGCAAAAAATTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTAATAA‑GGGTGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCt                                                                        <  5:331061/135‑1 (MQ=255)                                                               tataATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTg                                                                      >  7:211671/1‑137 (MQ=255)                                                                ataATGAAAAAAGAGTCTTCAAAAAAGTAAGTAAGTGATGATACGTATATAACATACAAAAAGGTTAATAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCa                                                                    <  8:337969/138‑1 (MQ=255)                                                                 taATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGCGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGt                                                                  >  4:300226/1‑139 (MQ=255)                                                                     gAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTg                                                                      <  4:304985/131‑1 (MQ=255)                                                                     gAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTg                                                                      >  3:304985/1‑131 (MQ=255)                                                                         aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     >  4:99814/1‑96 (MQ=255)                                                                         aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  3:99814/96‑1 (MQ=255)                                                                         aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     >  5:275344/1‑112 (MQ=255)                                                                         aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     >  5:280989/1‑112 (MQ=255)                                                                         aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  6:275344/112‑1 (MQ=255)                                                                         aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  6:280989/112‑1 (MQ=255)                                                                         aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCg                                                          >  3:292945/1‑139 (MQ=255)                                                                           agagTCTGCAAAAAAGTAAGTTAGTGATTATTCGTATCTAAAATTCATTAAGGTTTCTAA‑GGGGGGGATTCTATTGAGCGGTATTGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGt                                                        <  4:306421/139‑1 (MQ=255)                                                                            gagTCTGCAAAAAAGTTAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTa                                                       <  1:209336/139‑1 (MQ=255)                                                                                cTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAACAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTACCGGTTGCAGTGATTGGCGGTAAATg                                                   >  6:17082/1‑139 (MQ=255)                                                                                         agtaagtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCt                                          <  1:389700/139‑1 (MQ=255)                                                                                          gtacgtaactGATGATACGAATCTAACATACAAAAAGGTTACTAA‑GGGGGGGAGTCTATTGAGCCGGATGGACGACAAACGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTc                                         <  8:274806/139‑1 (MQ=255)                                                                                                 agtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGGTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTa                                   <  3:326236/138‑1 (MQ=255)                                                                                                   ttaagatACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGCGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATgg                                <  7:2426/135‑1 (MQ=255)                                                                                                     atgatACGGATCTAACATACAAAAAGGGTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCa                              <  2:316942/139‑1 (MQ=255)                                                                                                       gatACGTATCTAACATACAAAAAGGTTACTAA‑‑GGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTgg                                                            <  1:245378/106‑1 (MQ=255)                                                                                                       gatACGTATCTAACATACAAAAAGGTTACTAA‑‑GGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTgg                                                            >  2:245378/1‑106 (MQ=255)                                                                                                        atACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTa                                   <  8:157918/131‑1 (MQ=255)                                                                                                        atACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTa                                   >  7:157918/1‑131 (MQ=255)                                                                                                           cGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAg                         >  5:205812/1‑138 (MQ=255)                                                                                                             tATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGc                                           >  2:81611/1‑118 (MQ=255)                                                                                                             tATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGc                                           <  1:81611/118‑1 (MQ=255)                                                                                                                   aCATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAaaga                  <  3:220814/137‑1 (MQ=255)                                                                                                                     aTACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAgg               >  5:66538/1‑138 (MQ=255)                                                                                                                      tACAAAAGGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             <  1:343137/139‑1 (MQ=255)                                                                                                                      tACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  3:57138/1‑139 (MQ=255)                                                                                                                      tACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  5:199758/1‑139 (MQ=255)                                                                                                                       aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGTTAAATGGAAAATGCt                                          >  2:59019/1‑109 (MQ=255)                                                                                                                       aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGATAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  2:155229/1‑138 (MQ=255)                                                                                                                       aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGCTAAATGGAAAATGCt                                          <  1:59019/109‑1 (MQ=255)                                                                                                                         aaaaaGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGTTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGTcaca           >  3:27450/1‑138 (MQ=255)                                                                                                                         aaaaaGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGcaca           >  4:231489/1‑138 (MQ=255)                                                                                                                           aaaGGTTACTAA‑TGGGGGGACTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGTCGGTAAATGGAAAATGGTCATTTTATGGCATTTAGGAAAAGAAGGCACaaaa        <  6:263020/139‑1 (MQ=255)                                                                                                                              ggTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACgg     >  7:298247/1‑139 (MQ=255)                                                                                                                                  aCTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTc  >  7:315641/1‑138 (MQ=255)                                                                                                                                  aCTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTc  >  3:110184/1‑138 (MQ=255)                                                                                                                                       |                                                                                                                                      TTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAGGGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTC  >  BS168_reference_genome/375884‑376151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑