Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,097,087	(A)7→8	intergenic (‑111/+39)	azoJ ← / ← yocK	FMN‑dependent NADH‑azoreductase/putative general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,097,080	1	.	A	100.0%	128.8 / NA	38	intergenic (‑104/+46)	azoJ/yocK	FMN‑dependent NADH‑azoreductase/putative general stress protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (19/19);  total (19/19)

AATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTC‑‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATA  >  BS168_reference_genome/2096960‑2097202                                                                                                                          |                                                                                                                            aaTAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCAAAAAAAAAGCAAGTACa                                                                                                            <  2:88333/139‑1 (MQ=255)     aaaCTGTAGACATTTGTATTACTCCAGGTAAACTTTATATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAATTACATCTaa                                                                                                       <  5:17488/139‑1 (MQ=255)     aaaCTGTAGACATCTGTATTCCTCCCGGTAATCTATTTATATTTACTTACAAATAGTAGGTTACTTTCATTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTaa                                                                                                       <  6:395309/139‑1 (MQ=255)        cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAatat                                                                                                    <  6:107830/139‑1 (MQ=255)        cTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAatat                                                                                                    <  7:334732/139‑1 (MQ=255)                   tGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTActtg                                                                                         >  7:268667/1‑139 (MQ=255)                          ctccCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCa                                                                                  >  8:77075/1‑139 (MQ=255)                            cccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtt                                                                                >  1:377553/1‑139 (MQ=255)                            cccGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCAtt                                                                                >  8:374647/1‑139 (MQ=255)                                      tttttATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGTTGTACTTGCTTGGCATTTTTACTTCGa                                                                      <  2:377553/139‑1 (MQ=255)                                                 acttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa                                                            <  8:268667/138‑1 (MQ=255)                                                 acttacAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGCACTTGCTTGGCATTTTTACTTCGAATCTTGTTCa                                                            <  8:371889/138‑1 (MQ=255)                                                          tAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGa                                                  <  4:395702/139‑1 (MQ=255)                                                             tATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATcc                                                      <  3:88020/132‑1 (MQ=255)                                                             tATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATcc                                                      >  4:88020/1‑132 (MQ=255)                                                             tATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGAtt                                                >  8:374032/1‑138 (MQ=255)                                                               tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCa                                             >  5:123036/1‑139 (MQ=255)                                                               tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCa                                             >  3:106707/1‑139 (MQ=255)                                                               tGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCa                                             >  2:88063/1‑139 (MQ=255)                                                                 ttACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGa                                                  <  2:269495/132‑1 (MQ=255)                                                                 ttACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGa                                                  >  1:269495/1‑132 (MQ=255)                                                                    ctttctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGt                                        >  8:250761/1‑139 (MQ=255)                                                                       tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATcc                                                      >  2:335910/1‑122 (MQ=255)                                                                       tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATcc                                                      <  1:335910/122‑1 (MQ=255)                                                                       tctttTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACTTCTg                                     >  4:152774/1‑139 (MQ=255)                                                                         ttttttACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGc                                    >  1:206250/1‑138 (MQ=255)                                                                            tttACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAg                                 <  7:94138/138‑1 (MQ=255)                                                                                          aTCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAAtt                  >  1:255997/1‑139 (MQ=255)                                                                                           tCACTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTg                 >  4:134843/1‑139 (MQ=255)                                                                                              cTTTATTTTGAATATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGttt              <  2:255997/139‑1 (MQ=255)                                                                                                   ttttGAATATGAGATTTTTATTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTttg         <  2:348123/139‑1 (MQ=255)                                                                                                        aaTATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGa    >  5:393779/1‑139 (MQ=255)                                                                                                        aaTATGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGa    >  3:385348/1‑139 (MQ=255)                                                                                                         atatGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGa    >  1:309855/1‑138 (MQ=255)                                                                                                          tatGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa  <  7:77075/139‑1 (MQ=255)                                                                                                          tatGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa  <  3:225535/139‑1 (MQ=255)                                                                                                          tatGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa  <  3:181026/139‑1 (MQ=255)                                                                                                          tatGAGATTTTTTTTCA‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATa  <  2:206250/139‑1 (MQ=255)                                                                                                                          |                                                                                                                            AATAAAACTGTAGACATTTGTATTCCTCCCGGTAAACTTTTTATATTTACTTACAAATAGTATGTTACTTTCTTTTTTACGTAAAGTCAATCACTTTATTTTGAATATGAGATTTTTTTTC‑‑AAAAAAAGCAAGTACATCTAATATCGGTGTACTTGCTTGGCATTTTTACTTCGAATCTTGTTCACGATCCAAGATTTCATACGTCTGCGAGCTTTTTTGATCAATTGTTTCTTTGTTGGATA  >  BS168_reference_genome/2096960‑2097202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑