Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,345	Δ1 bp	intergenic (+17/‑155)	trnI‑Ala → / → rrnH‑16S	tRNA‑Ala/ribosomal RNA‑16S

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,344	0	G	.	100.0%	83.2 / NA	43	intergenic (+16/‑156)	trnI‑Ala/rrnH‑16S	tRNA‑Ala/ribosomal RNA‑16S
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (22/21);  total (22/21)

CATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTA  >  BS168_reference_genome/166208‑166462                                                                                                                                         |                                                                                                                       cATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgt                                                                                                                       >  7:189819/1‑137 (MQ=18)   tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat                                                                                                                    >  3:338796/1‑135 (MQ=17)   tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgatat                                                                                                                    >  3:262630/1‑135 (MQ=18)        tCGCAGGTTCGAATCCTGTCTTCCCTACCATTTTTTATGGGGCCTTATCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATAAAGCTATGCTCCACCAAAAGTTTTTAAATAA‑GTTATTGACt                                                                                                              <  1:130695/139‑1 (MQ=11)        tCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACt                                                                                                              >  7:116879/1‑139 (MQ=17)         cGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACtt                                                                                                             >  2:217191/1‑139 (MQ=17)            aGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTga                                                                                                          <  2:174814/139‑1 (MQ=17)            aGGTGCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTga                                                                                                          <  8:85024/139‑1 (MQ=12)                  gAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGGTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGGTCCACCAAAAGTTTTTAAATAA‑GTTATTGACTTTGAAGAAGt                                                                                                    >  7:231740/1‑139 (MQ=11)                  gAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGt                                                                                                    >  7:335091/1‑139 (MQ=17)                   aaTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg                                                                                                   <  1:177785/139‑1 (MQ=17)                    aTCCTGTCTTCCTGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGa                                                                                                  <  2:338592/139‑1 (MQ=12)                    aTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGa                                                                                                  <  4:278010/139‑1 (MQ=17)                       cTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACa                                                                                                <  7:243150/138‑1 (MQ=18)                       cTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACa                                                                                                <  7:221106/138‑1 (MQ=18)                       cTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACa                                                                                                <  2:161639/138‑1 (MQ=18)                       cTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACa                                                                                                <  6:251441/138‑1 (MQ=18)                        tGTCTTCCAGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACAtt                                                                                              >  8:209293/1‑139 (MQ=255)                            ttCCCTACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCTGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta                                                                                           <  1:345328/138‑1 (MQ=255)                              cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGCGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATACCGCTAGGCTCCCCCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGATGAAGTGACATTGTAGAc                                                                                        >  2:69391/1‑139 (MQ=11)                              cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGtat                                                                                          <  8:116879/137‑1 (MQ=21)                              cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta                                                                                           <  7:97949/136‑1 (MQ=21)                              cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATAc                                                                                        >  6:336251/1‑139 (MQ=21)                              cccGAACATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta                                                                                           <  2:82396/136‑1 (MQ=255)                                     aTTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATaaa                                                                                 <  8:75877/139‑1 (MQ=14)                                     aTTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATaaa                                                                                 <  7:297424/139‑1 (MQ=14)                                                           aGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACt                                                                                       >  6:273990/1‑111 (MQ=11)                                                           aGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACt                                                                                       <  5:273990/111‑1 (MQ=11)                                                                       gcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAGCt                                               >  6:10629/1‑139 (MQ=2)                                                                               tGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaaaa                                        <  1:8517/138‑1 (MQ=11)                                                                                   cGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTaaa                                   <  5:65804/139‑1 (MQ=11)                                                                                      aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                  >  7:188241/1‑137 (MQ=11)                                                                                      aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                  >  7:114766/1‑137 (MQ=11)                                                                                      aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                  >  5:111679/1‑137 (MQ=11)                                                                                      aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc                                  >  7:316589/1‑137 (MQ=11)                                                                                        gaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGa                              <  8:189819/139‑1 (MQ=11)                                                                                              aGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAAc                        >  2:245976/1‑139 (MQ=11)                                                                                                cGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACg                       >  1:345333/1‑138 (MQ=11)                                                                                                cGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACg                       >  8:195799/1‑138 (MQ=11)                                                                                                cGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACg                       >  4:61222/1‑138 (MQ=11)                                                                                                     cGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAATGCGGCCACACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTg                 >  7:4116/1‑139 (MQ=11)                                                                                                     cGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAGCTTTGAAAACTAAACAAGACAAAACGTACCTg                 >  2:314142/1‑139 (MQ=2)                                                                                                           cGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAtt           <  7:195799/139‑1 (MQ=11)                                                                                                             cTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAACACGTACCTGTTAAtt           >  4:327181/1‑137 (MQ=2)                                                                                                                     caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa  >  3:178443/1‑138 (MQ=11)                                                                                                                     caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGAACCTGTTAATTCAGTTTTTa  >  3:94081/1‑138 (MQ=2)                                                                                                                                         |                                                                                                                       CATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTA  >  BS168_reference_genome/166208‑166462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑