| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | BS168_reference_genome | 1,317,152 | +GT | intergenic (+23/+5) | phrA → / ← yjpA | secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,317,152 | 1 | . | G | 96.2% | 194.3 / 0.5 | 54 | intergenic (+23/+5) | phrA/yjpA | secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (25/26); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
| * | BS168_reference_genome | 1,317,152 | 2 | . | T | 94.3% | 150.5 / 3.2 | 54 | intergenic (+23/+5) | phrA/yjpA | secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base T (24/26); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑G‑‑C‑TTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGC > BS168_reference_genome/1317023‑1317273 || tgCTCGTTGCGGTCGGGTTGAGCTTTTCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTTCGGCACGCAATCAAACATGATGCAAAAAAAAAGACCCTTAGG‑G‑‑G‑TCtttttt > 1:158574/1‑139 (MQ=255) cGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑G‑‑G‑TCttttttattt > 8:84656/1‑135 (MQ=255) gTTCAGCTTTACTCAGGTGATGGGTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCa < 3:126098/139‑1 (MQ=255) aCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGTGT‑CTTTTTTATTTCTTCAGCTTCCATTCTTTTATc > 4:225879/1‑139 (MQ=255) cTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCTGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGATTCCATTCTTTTATCg > 2:308744/1‑139 (MQ=255) cAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt > 5:90906/1‑138 (MQ=255) aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt < 7:219382/137‑1 (MQ=255) aGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt < 7:83679/137‑1 (MQ=255) gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGc > 5:259779/1‑139 (MQ=255) gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGc > 6:39044/1‑139 (MQ=255) gATGGATCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc < 2:188117/139‑1 (MQ=255) ggTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCag > 4:30528/1‑138 (MQ=255) ggTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTC‑TTTTTTATTCTTCAGCTTCCATTCTTTTATCGTCAGCTCag > 3:318641/1‑137 (MQ=255) cATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCagaaga < 4:158642/138‑1 (MQ=255) aTGCAGGTGAAACAGCAAACACAGCAGGGAAAACATTACATATATCGGCACGCAATCAAACATGATCCATAAAAAAAGACCCTAAGG‑GGACTTTTTTATTACTTCAGCTTCCATTCTTATATCTTCATATCTGAAGATc > 8:158418/1‑139 (MQ=255) aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt > 8:275951/1‑125 (MQ=255) aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGt < 7:275951/125‑1 (MQ=255) aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc > 6:235073/1‑139 (MQ=255) aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc > 7:72008/1‑139 (MQ=255) aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc > 3:331771/1‑139 (MQ=255) aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATc > 7:59853/1‑139 (MQ=255) taaaCAGCAAACACAGCATGGAAAACAATTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGcc < 8:203909/138‑1 (MQ=255) aaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGccac < 7:250252/139‑1 (MQ=255) aaaCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTTTTTATTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATACACTTGcccccccccg > 2:173251/1‑134 (MQ=255) aaCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGa < 1:173251/139‑1 (MQ=255) acacAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATCATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGAt < 3:263583/139‑1 (MQ=255) gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa > 2:97259/1‑139 (MQ=255) gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa < 1:97259/139‑1 (MQ=255) ttCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATgg < 7:346422/129‑1 (MQ=255) ttCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATgg > 8:346422/1‑129 (MQ=255) ttCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg > 3:181205/1‑139 (MQ=255) tatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGc < 3:70112/139‑1 (MQ=255) tGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt < 4:42025/134‑1 (MQ=255) tGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt > 3:42025/1‑134 (MQ=255) gCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCctct > 1:264863/1‑139 (MQ=255) aTCAAACATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAAt < 1:80437/139‑1 (MQ=255) aCATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCt < 3:116023/139‑1 (MQ=255) cATGATGCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCtt < 6:63971/139‑1 (MQ=255) tgattCATAAAAAAAGACCCCTAGG‑GGTCTTTTTTATTTCTTCAGCCTACATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg < 1:308744/139‑1 (MQ=255) tgatgCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg < 3:59375/139‑1 (MQ=255) tgatgCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg < 8:59853/139‑1 (MQ=255) tgatgCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg < 8:72008/139‑1 (MQ=255) gatgCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGa > 1:232735/1‑139 (MQ=255) gatgCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGa > 5:293555/1‑139 (MQ=255) gatgCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGa > 5:236982/1‑139 (MQ=255) atgCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt < 2:158574/139‑1 (MQ=255) tgCATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGAGCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt > 1:79842/1‑138 (MQ=255) cATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg > 7:177324/1‑134 (MQ=255) cATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg < 8:177324/134‑1 (MQ=255) cATAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGa > 6:126705/1‑138 (MQ=255) aTAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACa < 2:232735/139‑1 (MQ=255) tAAAAAAAGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATACACTTGCCACCCGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACa > 6:332572/1‑138 (MQ=255) aaaaaaGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATa < 6:236982/138‑1 (MQ=255) aaaaGACCCTTAGG‑GGTCTTTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGc < 1:128725/139‑1 (MQ=255) || TGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGG‑G‑‑C‑TTTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGC > BS168_reference_genome/1317023‑1317273 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |