Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	165,826	+C	intergenic (+1/‑4)	trnI‑Asn → / → trnI‑Thr	tRNA‑Asn/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,825	1	.	C	97.8%	296.4 / 0.2	89	noncoding (72/72 nt)	trnI‑Asn	tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (51/36);  total (52/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

TAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTG  >  BS168_reference_genome/165691‑165952                                                                                                                                        |                                                                                                                                tAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccattg                                                                                                                           >  7:173428/1‑134 (MQ=21)   ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                                                                                                           <  8:173428/138‑1 (MQ=21)     aCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTc                                                                                                                        >  8:231004/1‑139 (MQ=21)       gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                      <  8:283373/139‑1 (MQ=21)       gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                      >  2:40092/1‑139 (MQ=21)       gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                      <  1:6177/139‑1 (MQ=21)         cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                      >  5:339193/1‑137 (MQ=21)         cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                      >  1:183562/1‑137 (MQ=21)         cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                      >  8:213720/1‑137 (MQ=21)                ccccattataaATCCTACCCAGTTTGTATATCAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                                                                                                             <  6:284351/134‑1 (MQ=14)                cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                                                                                                             <  6:199250/139‑1 (MQ=21)                cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                                                                                                             <  3:105779/139‑1 (MQ=21)                cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                                                                                                             <  3:327715/139‑1 (MQ=21)                 aGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagca                                                                                                            <  7:96314/139‑1 (MQ=21)                   aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                                                                                                          >  2:29394/1‑139 (MQ=21)                        ttaATTCTTCCATGTTTGTTTAAGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGCGCTATCGGCTGTTAACCGATCGGGCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                     <  8:148703/139‑1 (MQ=18)                        ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                     <  7:231004/139‑1 (MQ=21)                             tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                >  6:101502/1‑139 (MQ=21)                             tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                >  1:299868/1‑139 (MQ=21)                             tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                >  1:16278/1‑139 (MQ=21)                                 ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                             <  8:84602/138‑1 (MQ=21)                                 ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                             <  8:176273/138‑1 (MQ=21)                                 ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                             <  1:29394/138‑1 (MQ=21)                                         gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAgg                                                                                     >  4:119014/1‑138 (MQ=21)                                          tttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                   <  1:280910/139‑1 (MQ=21)                                                aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATa                                                                                                                    >  5:78555/1‑100 (MQ=11)                                                aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATa                                                                                                                    <  6:78555/100‑1 (MQ=11)                                                aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                   >  7:238263/1‑133 (MQ=18)                                                aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                   <  8:238263/133‑1 (MQ=18)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                     <  1:219409/102‑1 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                     >  2:219409/1‑102 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGCGcc                                                                >  3:198896/1‑139 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  1:281960/1‑139 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  1:53826/1‑139 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  8:29489/1‑139 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  7:214280/1‑139 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  4:184187/1‑139 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGGAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  4:107861/1‑139 (MQ=255)                                                               ttCCGCAGTAGCTCAGTGGTAGAGCAATAGGCTGTTAACCGATCGGTCGTAGGTTCCAAACCAAACTGCCGAGCCATTTCTTCCATAGCTCACCAGGTAGAGCACTTCCATGGGAATGGAGAGGTCAGCCGATCCAGcc                                                                >  2:192728/1‑139 (MQ=17)                                                                    cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                      >  1:236843/1‑80 (MQ=9)                                                                    cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                      <  2:236843/80‑1 (MQ=9)                                                                    cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATgg                                                                                          <  7:250364/108‑1 (MQ=255)                                                                    cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATgg                                                                                          >  8:250364/1‑108 (MQ=255)                                                                    cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCtt                                                           >  4:304943/1‑139 (MQ=255)                                                                    cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCtg                                                           >  1:311490/1‑138 (MQ=255)                                                                      gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                         <  7:213720/139‑1 (MQ=255)                                                                      gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                         <  2:53826/139‑1 (MQ=255)                                                                           gcAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCt                                                    <  6:219720/138‑1 (MQ=255)                                                                             aGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCt                                                    <  5:101502/137‑1 (MQ=255)                                                                               tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                >  8:111903/1‑139 (MQ=255)                                                                               tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                >  2:193362/1‑139 (MQ=255)                                                                               tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTa                                                  >  2:140896/1‑137 (MQ=255)                                                                               tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTa                                                  <  1:140896/137‑1 (MQ=255)                                                                               tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTAAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                >  7:227955/1‑139 (MQ=255)                                                                                 gTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATg                                              >  8:179854/1‑139 (MQ=255)                                                                                   agagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGta                                            >  3:189761/1‑139 (MQ=255)                                                                                    gagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                <  6:177215/118‑1 (MQ=255)                                                                                    gagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  5:177215/1‑118 (MQ=255)                                                                                    gagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtat                                           <  1:193362/139‑1 (MQ=255)                                                                                     agCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtat                                           >  4:296328/1‑138 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCTAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  8:347783/1‑139 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                             >  4:238416/1‑84 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                             <  3:238416/84‑1 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  5:43330/1‑139 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  4:202028/1‑139 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  8:55479/1‑139 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  3:274625/1‑139 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  3:294929/1‑139 (MQ=255)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CAt                                                                                                                              >  4:57709/1‑50 (MQ=35)                                                                                         aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CAt                                                                                                                              <  3:57709/50‑1 (MQ=35)                                                                                                 ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa                                                                                        <  7:175044/81‑1 (MQ=255)                                                                                                 ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa                                                                                        >  8:175044/1‑81 (MQ=255)                                                                                                 ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                               <  7:111903/138‑1 (MQ=255)                                                                                                   aaCCGATCGGTCGTAGGGTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGttt                             <  6:45038/138‑1 (MQ=255)                                                                                                      cGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCa                         >  4:337268/1‑139 (MQ=255)                                                                                                              cGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                 <  2:183562/139‑1 (MQ=255)                                                                                                              cGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                 <  3:184187/139‑1 (MQ=255)                                                                                                               gTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                 <  6:339193/138‑1 (MQ=255)                                                                                                                  ggTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGc             >  3:325711/1‑139 (MQ=255)                                                                                                                       gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTGCCAAGGTTTCTCATAAGGAGAAAGCttttt        >  3:305573/1‑139 (MQ=255)                                                                                                                       gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttttt        >  3:114302/1‑139 (MQ=255)                                                                                                                       gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttttt        >  7:299842/1‑139 (MQ=255)                                                                                                                       gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttttt        >  2:5039/1‑139 (MQ=255)                                                                                                                       gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttttt        >  6:50100/1‑139 (MQ=255)                                                                                                                       gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACAAAGGTTTCTCATAAGGAGAAAGCttttt        >  8:192316/1‑139 (MQ=255)                                                                                                                              aCCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCTGCTTGGAAGCtt                                                   <  2:281245/89‑1 (MQ=255)                                                                                                                              aCCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCGGCTTGGAAGCtt                                                   >  1:281245/1‑89 (MQ=255)                                                                                                                               ccTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTg  <  2:281960/137‑1 (MQ=255)                                                                                                                                   cGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGc                                                                 >  8:101405/1‑70 (MQ=255)                                                                                                                                   cGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGc                                                                 <  7:101405/70‑1 (MQ=255)                                                                                                                                        |                                                                                                                                TAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTG  >  BS168_reference_genome/165691‑165952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑