Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,580,038	(T)9→8	intergenic (+43/‑83)	bshC → / → mraZ	malate glucosamine cysteine ligase/inhibitor of RsmH and transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,580,030	0	T	.	100.0%	250.2 / NA	55	intergenic (+35/‑91)	bshC/mraZ	malate glucosamine cysteine ligase/inhibitor of RsmH and transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/36);  total (19/36)

TCATGTATTATTTGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGTTTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATCATGTTTATGGGTGAATATCAGCATACCATCGATGCGAAAGG  >  BS168_reference_genome/1579898‑1580161                                                                                                                                     |                                                                                                                                    tCATGTATTATTTGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGttttttt                                                                                                                               >  1:41360/1‑139 (MQ=255) tCATGTATTATTTGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGttttttt                                                                                                                               >  1:44246/1‑139 (MQ=255) tCATGTATTATTTGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGttttttt                                                                                                                               <  2:41360/139‑1 (MQ=255)   aTGTATTATTTGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGtttttttta                                                                                                                             <  6:196463/139‑2 (MQ=255)   aTGTATTATTTGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGtttttttta                                                                                                                             <  8:214074/139‑2 (MQ=255)   aTGTATTATTTGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGtttttttta                                                                                                                             <  2:232617/139‑2 (MQ=255)           tttGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTaaa                                                                                                                    >  5:116889/1‑139 (MQ=255)           tttGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTaa                                                                                                                     >  6:267765/1‑138 (MQ=255)           tttGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTACAGGG‑TTTTTTTTATGGGTaaa                                                                                                                    >  7:291914/1‑139 (MQ=255)           tttGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTATCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTaaa                                                                                                                    <  7:76975/139‑1 (MQ=255)              gAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCACGG‑TTTTTTTTATTGGTAAACAg                                                                                                                 >  8:246511/1‑139 (MQ=255)                aCAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAgtg                                                                                                               >  8:179727/1‑139 (MQ=255)                aCAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAgtg                                                                                                               >  5:310968/1‑139 (MQ=255)                aCAGGTACGGTCCAAAATTCATCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTTTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAgtg                                                                                                               <  7:214338/139‑1 (MQ=255)                    gTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTaa                                                                                                            >  2:272383/1‑138 (MQ=255)                       cGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATg                                                                                                        <  4:214374/139‑1 (MQ=255)                         gTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATg                                                                                                        >  3:123936/1‑137 (MQ=255)                          tCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAg                                                                                                     <  1:272383/139‑1 (MQ=255)                              aaaTTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAg                                                                                                     <  8:29947/135‑1 (MQ=255)                              aaaTTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAg                                                                                                     >  1:85868/1‑135 (MQ=255)                              aaaTTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAg                                                                                                     <  2:85868/135‑1 (MQ=255)                              aaaTTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAg                                                                                                     >  7:29947/1‑135 (MQ=255)                              aaaTTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTtgt                                                                                                 >  1:194904/1‑139 (MQ=255)                                    ttcACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGc                                                                                            >  6:85684/1‑138 (MQ=255)                                    ttcACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGc                                                                                            >  7:95582/1‑138 (MQ=255)                                      cACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGtttttttta                                                                                                                             <  8:177589/104‑2 (MQ=255)                                      cACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGtttttttta                                                                                                                             >  7:177589/1‑103 (MQ=255)                                      cACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCg                                                                                           <  5:73177/137‑1 (MQ=255)                                      cACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCg                                                                                           <  1:56583/137‑1 (MQ=255)                                      cACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCg                                                                                           <  6:310968/137‑1 (MQ=255)                                       aCGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGaa                                                                                         <  7:246511/138‑1 (MQ=255)                                       aCGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGaa                                                                                         <  2:97980/138‑1 (MQ=255)                                       aCGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGaa                                                                                         <  2:55093/138‑1 (MQ=255)                                       aCGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGaa                                                                                         <  5:85684/138‑1 (MQ=255)                                       aCGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAg                                                                                        <  7:110186/139‑1 (MQ=255)                                         gACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAgtg                                                                                      <  2:40363/139‑1 (MQ=255)                                              tCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAg                                                                                                     >  7:96166/1‑119 (MQ=255)                                              tCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAg                                                                                                     <  8:96166/119‑1 (MQ=255)                                               cAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGaa                                                                                 <  7:130684/138‑1 (MQ=255)                                               cAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGaa                                                                                 <  5:76527/138‑1 (MQ=255)                                                   aaTCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtg                                                                            <  6:116889/139‑1 (MQ=255)                                                   aaTCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtg                                                                            <  6:45050/139‑1 (MQ=255)                                                    aTCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtg                                                                            <  4:123936/138‑1 (MQ=255)                                                       tGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTa                                                                                                             <  7:181300/102‑1 (MQ=255)                                                       tGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTa                                                                                                             >  8:181300/1‑102 (MQ=255)                                                       tGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtggt                                                                          >  5:275337/1‑137 (MQ=255)                                                       tGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtggt                                                                          >  8:25686/1‑137 (MQ=255)                                                         ccATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGTGAAGTGGTGAATAgtggt                                                                          <  2:128765/135‑1 (MQ=255)                                                         ccATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtggt                                                                          <  8:95582/135‑1 (MQ=255)                                                                                  aaGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAgaga                                             <  8:257694/139‑1 (MQ=255)                                                                                    gTTGTCAAACTTTGAAATAAAGTTTTATAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATc                                           <  2:38683/139‑1 (MQ=255)                                                                                    gTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATc                                           <  2:44246/139‑1 (MQ=255)                                                                                    gTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGGGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATc                                           <  5:267765/139‑1 (MQ=255)                                                                                           aaCTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATCATGTTTa                                    <  7:179727/139‑1 (MQ=255)                                                                                                  aaaTAAAGTTTTAAAGAACCCTGACTAGTTCAGTG‑TTTTTTTTATGTGTATACAGTGTAAAATGAAGTTGCGGAGCGTAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATCATGTTTATGGGTg                              <  4:98002/138‑1 (MQ=255)                                                                                                       aaGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtggt                                                                          >  2:205493/1‑89 (MQ=255)                                                                                                       aaGTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtggt                                                                          <  1:205493/89‑1 (MQ=255)                                                                                                         gTTTTAAAGAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATTAAGTTGTGGAGCTAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGATATCATGTTTATGGGTGAATATCAg                      <  8:263043/139‑1 (MQ=255)                                                                                                                 gAACCCTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATCATGTTTATGGGTGAATATCAGCATACCAt              <  4:33943/139‑1 (MQ=255)                                                                                                                    cccTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtggt                                                                          >  3:262405/1‑76 (MQ=255)                                                                                                                    cccTGACTAGTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAgtggt                                                                          <  4:262405/76‑1 (MQ=255)                                                                                                                             gTTCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATCATGTTTATGGGTGAATATCAGCATACCATCGATGCGAAAgg  <  5:99594/139‑1 (MQ=255)                                                                                                                              ttCAGGG‑TTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATCATGTTTATGGGTGAATATCAGCATACCATCGATGCGAAAgg  <  8:291914/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    TCATGTATTATTTGAACAGGTACGGTCCAAAATTCTTCACGACATTCAAGAATCTGCCATTTTCTTTTCAAAATCAACATCAAGTTGTCAAACTTTGAAATAAAGTTTTAAAGAACCCTGACTAGTTCAGGGTTTTTTTTTATGGGTAAACAGTGTAAAATGAAGTTGTGGAGCGAAGTGGTGAATAGTGGTGAGTTAAGGAGAGAAAGTGGGGCAAGAGATCATGTTTATGGGTGAATATCAGCATACCATCGATGCGAAAGG  >  BS168_reference_genome/1579898‑1580161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑