Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,037	+T	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,037	1	.	T	95.5%	184.9 / 1.6	67	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base T (24/40);  total (27/40)
Fisher's exact test for biased strand distribution p-value = 6.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑A‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAA  >  BS168_reference_genome/165905‑166166                                                                                                                                         |                                                                                                                                  aaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTaa                                                                                                                              <  6:225322/139‑1 (MQ=255) aaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTaa                                                                                                                              <  3:307482/139‑1 (MQ=255)      attattACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACggg                                                                                                                          <  1:157459/138‑1 (MQ=255)      attattACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACggg                                                                                                                          <  7:130793/138‑1 (MQ=255)       ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGa                                                                                                                         >  3:152993/1‑138 (MQ=255)       ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCGAACGGGat                                                                                                                        >  7:41544/1‑139 (MQ=255)       ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGa                                                                                                                         <  5:4705/138‑1 (MQ=255)           taCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGGCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTg                                                                                                                    >  8:195472/1‑139 (MQ=255)            aCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATACCGTCGTCCGCGCC‑A‑TTTAA‑CATAACGGGATAttt                                                                                                                    >  2:106244/1‑137 (MQ=21)            aCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTAGAAAGCCGGCGTCCGCTCC‑C‑TTT‑ATACTTACGGGATATTGt                                                                                                                   >  2:236869/1‑139 (MQ=21)                 ggTTTCTCATAAGGAGAAAGCGTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGCGGGTCTCGGGTTCGAATCCCGTATTGCTCGAC‑A‑GTT‑ATAATAACAGGATAATGTAaatt                                                                                                              >  6:130787/1‑139 (MQ=18)                     tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAaattgaat                                                                                                          >  6:89850/1‑139 (MQ=255)                     tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTACGCTCC‑C‑TTT‑ATACTAAGGGGATATTTTAaatttatt                                                                                                          >  6:27798/1‑135 (MQ=18)                      ctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGAGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCATAACGGGATATTGTAaattgaatt                                                                                                         >  8:326822/1‑139 (MQ=255)                       tcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAaattgaattg                                                                                                        >  7:109470/1‑139 (MQ=255)                         aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTgc                                                                                                       <  8:85556/138‑1 (MQ=255)                            aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGTTATTGTAAATTGAATTGCGccc                                                                                                   >  7:207485/1‑139 (MQ=255)                                gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                               <  1:314423/139‑1 (MQ=255)                                gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                               <  8:316047/139‑1 (MQ=255)                                 aaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGc                                                                                              >  8:28314/1‑139 (MQ=255)                                  aggCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             <  5:277535/137‑1 (MQ=255)                                  aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             <  3:94704/139‑1 (MQ=255)                                  aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             <  5:8034/139‑1 (MQ=255)                                  aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             <  7:28314/139‑1 (MQ=255)                                  aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             <  2:237015/139‑1 (MQ=255)                                  aaGATTTTTTTATTGCGAAATGCGGAAGTAGTGCAGTGGAAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             <  4:12662/139‑1 (MQ=21)                                   aGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATGCAAACGGGATATTGTATATTGAAATGCGCCCGTAGCt                                                                                             >  8:194336/1‑138 (MQ=18)                                   aGCTTTTTTTATTGCGATATGCGGAAGTAGATCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAAACCCGTCTTCCGCTCCAA‑TTA‑A‑CCTAACGGGATATTTTAAATTGAATTGAGCCCGTAGCt                                                                                             >  6:332523/1‑138 (MQ=18)                                    gCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCa                                                                                           <  8:109470/139‑1 (MQ=255)                                     cTTTTTTTATTGCGATATGCGGAAGTAGTTCATTGGTAGAACACCACCTTGCCCAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                          <  5:130787/139‑1 (MQ=25)                                     cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                          <  5:89850/139‑1 (MQ=255)                                     cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                          <  3:120143/139‑1 (MQ=255)                                     cTTTTGTTATTGCGATATGCGGTAGTAGTTCAGTGGTAGAACACCACCTTTCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGTATTGCGCCCGTAGCTCaa                                                                                          <  1:180529/139‑1 (MQ=21)                                           ttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGa                                                                                     <  3:154629/138‑1 (MQ=255)                                             aTTGCGATATGCGGAATTAGTTCAGTGGTAGAACACCACCTTGCCATGGTGGGGGTCGCGGGTTCGATTCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                                   <  5:332523/138‑1 (MQ=25)                                               tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTGCCCGGC‑A‑TTT‑ATCCTAAAGGGATATTTTAAATTTAATTGCGCCCGTAGCTGAAATGGATagct                                                                                >  6:277535/1‑137 (MQ=18)                                               tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCAAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATagag                                                                                >  7:323516/1‑139 (MQ=255)                                                          gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTAc                                                                     <  5:219654/139‑1 (MQ=255)                                                          gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTAc                                                                     <  7:217324/139‑1 (MQ=255)                                                           aaGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTGCGCGCC‑A‑TTT‑ATCCGTACGGGATATTGGAAATTGAATTGCGCCCGGAGCTCAATTGGATAGAGCGGTTGACTaag                                                                    >  8:65639/1‑137 (MQ=21)                                                                gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCCATTGGATAGAGCGTTTGACTACGGATc                                                                >  3:152863/1‑138 (MQ=255)                                                                gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTAGAATCCCGTCTTCCGCTCC‑ATTTT‑T‑ACTAACGGGATATTTTAAATTTAATTTCGCCCGTAGCTCAATTGGATAGAGCGGTTGCCGACGGATc                                                                >  2:11511/1‑138 (MQ=18)                                                                 ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                              <  8:50987/139‑1 (MQ=255)                                                                 ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                              <  4:152993/139‑1 (MQ=255)                                                                 ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTGCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                              <  6:39333/139‑1 (MQ=255)                                                                 ttCAGTGGTACAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAAGCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                              <  4:43812/139‑1 (MQ=255)                                                                  tCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                              <  1:301634/138‑1 (MQ=255)                                                                        gTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGtt                                                        <  3:108104/138‑1 (MQ=255)                                                                        gTAGAACACCACATTGCCACGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATAGCGCCCGTAGCTCACTTGGATAGAGCGTTTGACTACGGATCAAAAGGtt                                                        <  6:304734/138‑1 (MQ=37)                                                                         tAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAg                                                      <  1:236869/139‑1 (MQ=255)                                                                         tAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAg                                                      <  5:200480/139‑1 (MQ=255)                                                                          aGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATACTAAAGGGATATTTTAAAATGAATTGCGCCCGTAGCTCAATTGGATATAGCGTTTGACTAAGGATCAAAAGGTTaag                                                     >  8:332194/1‑137 (MQ=25)                                                                                ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTTCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCg                                               >  2:227177/1‑139 (MQ=255)                                                                                ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCg                                               >  4:99995/1‑139 (MQ=255)                                                                                ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCGCC‑A‑TTT‑ATGCTAACGGGATATTGTAAATTGAATTGCGCCCGTTGCGCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCg                                               >  8:46823/1‑139 (MQ=37)                                                                                 caccTTGCCAAGGTGGGGGTCGCGTGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGACCGTAGCTCAATTGGATAGAGCGTTTGACTAGGGATCAAAAGGTTAGGGGTTCGa                                              <  8:99997/139‑1 (MQ=37)                                                                                 caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                              <  3:99995/139‑1 (MQ=255)                                                                                 caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                              <  1:227177/139‑1 (MQ=255)                                                                                 caccTTGCCAAGGTGGGGGTCGCGGGGTGGAATCCCGTCTTGCGCTCC‑A‑TTT‑ATACTAACGGGATATTGTAAATTGAATTGAGCCCGTAGCACAATTGGATAGAGCGTTTTACTACGGTTCAAAAAGTTAGGGGttggc                                              >  6:281378/1‑136 (MQ=25)                                                                                   ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                            >  7:64079/1‑139 (MQ=255)                                                                                            ggTGGGGGTCGCGGGGGCGAATCCCGGCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCggg                                   <  6:33059/139‑1 (MQ=21)                                                                                                  ggTCGCGGGTTCGAATCCCCTCTTCCGGTAC‑A‑ATT‑ATCCTAACGGGAAATTGTTAATTTAATTGCGCCCGTAACACAATTGGATAGAGCGTTTGAATAAGGATCAAAAGGTTAGGGGTTCGACTCCGCACGGgcgccgc                             >  6:175098/1‑136 (MQ=18)                                                                                                   gTCGCGGGTGCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATAGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGGTCGACTCCTCTCGGGCGCGCCa                            <  7:293305/139‑1 (MQ=21)                                                                                                   gTCGCGGGATCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCa                            <  5:27798/139‑1 (MQ=255)                                                                                                          gTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCttt                     <  6:93617/139‑1 (MQ=255)                                                                                                                 tCCCGTCTTCCGCTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaataga              <  1:11511/139‑1 (MQ=255)                                                                                                                             cTCC‑A‑TTT‑ATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaaa  >  2:107569/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                  AATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCC‑A‑TTT‑A‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAA  >  BS168_reference_genome/165905‑166166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑