Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	121,760	T→C	intergenic (+87/‑159)	rlmG → / → rpoB	23S rRNA m2G1835 methyltransferase/RNA polymerase (beta subunit)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	121,760	0	T	C	100.0%	291.2 / NA	75	intergenic (+87/‑159)	rlmG/rpoB	23S rRNA m2G1835 methyltransferase/RNA polymerase (beta subunit)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/23);  total (52/23)

GTTCAAAAAAAGAAAGGCTATTATATCATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAATTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTACACATATAATAC  >  BS168_reference_genome/121626‑121895                                                                                                                                       |                                                                                                                                        gtTCAAAAAAAGAAAGGCTATTATATCATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACtata                                                                                                                                     <  1:133680/139‑1 (MQ=255)   tCAAAAAAAGAAAGGCTATTATATCATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATAc                                                                                                                                    <  1:232852/138‑1 (MQ=255)           agaaagGCTATTATATCATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTATAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGa                                                                                                                           <  6:97313/139‑1 (MQ=255)            gaaagGCTATTATATCATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGa                                                                                                                           >  7:178198/1‑138 (MQ=255)                ggCTATTATATCATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGc                                                                                                                      <  7:60100/139‑1 (MQ=255)                          tCATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAAt                                                                                                             <  7:82180/138‑1 (MQ=255)                           cATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCg                                                                                                           >  8:130730/1‑139 (MQ=255)                             tAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTAGACAAGTATGGAAATGCTAATATAATCgg                                                                                                          >  2:273225/1‑138 (MQ=255)                                      aaaaaGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGtttt                                                                                                >  4:22371/1‑139 (MQ=255)                                           gattGTCTCGGTATTTTAACTCTGTTAATAATGTAAACTGCCAATGTTTATATTTTGCTTGATATGCTGAATTGTCAATATCTATGTATAAACTATACAAGTATGGTAATGCTAATAAAATCGGTATTTGTTTTTGGAt                                                                                           <  2:144444/137‑1 (MQ=255)                                            tttGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGAtg                                                                                          >  4:236109/1‑139 (MQ=255)                                                      tATTTTAAATATGTTAATATTGTAAAATGCAAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATCCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCt                                                                                 >  4:34315/1‑138 (MQ=255)                                                               tATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGaaa                                                                       >  1:218611/1‑139 (MQ=255)                                                                aTGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAAc                                                                      >  7:112363/1‑139 (MQ=255)                                                                aTGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAAc                                                                      >  1:203875/1‑139 (MQ=255)                                                                 tGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTactgt                                                                       <  7:309982/137‑5 (MQ=255)                                                                  cttAAAAATGAAAAATGCCAATCAATATATTATGATTGATATGATGAATTCTCAAAATCTAACTAAAAACTAAACAAGTAAGGAAATGCTAATAAAATCGGAATATGTTTAAGAATCAGTTTATCTTAATTAGAAAcaa                                                                    >  4:70092/2‑137 (MQ=255)                                                                        atTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCtttttt                                                              >  1:315558/1‑139 (MQ=255)                                                                        atTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCtttttt                                                              >  5:116462/1‑139 (MQ=255)                                                                           gTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACGCTTTTTTCtt                                                           >  8:66811/1‑139 (MQ=255)                                                                           gTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCtt                                                           >  2:155244/1‑139 (MQ=255)                                                                           gTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCtt                                                           >  2:155246/1‑139 (MQ=255)                                                                           gTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCtt                                                           >  4:179810/1‑139 (MQ=255)                                                                            tAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGTATGTGGTTTTCTTAATTAGAAACCCTTTTTTCttt                                                          >  7:53040/1‑139 (MQ=255)                                                                                      aTGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTa                                                 >  7:95707/1‑138 (MQ=255)                                                                                              atTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTAttt                                        >  8:268434/1‑139 (MQ=255)                                                                                               ttttGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTAtttt                                       >  7:80842/1‑139 (MQ=255)                                                                                                tttGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttc                                      >  1:209726/1‑139 (MQ=255)                                                                                                tttGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTAAATTAGAAACCCTTTTTTCTTTTGTCTTGTTAAAGTATtttc                                      >  3:177030/1‑139 (MQ=255)                                                                                                 ttGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTGTTTTGTCTTGTAAAAGTATtttct                                     >  1:152682/1‑139 (MQ=255)                                                                                                  tGCTTGATATGATGATTTGTCTATATCTATGTATAAACTATACAAGTTTTGTAATGCTAATAATATCTGTATTTGTTTTTGGATTTTGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttct                                     <  3:70092/138‑1 (MQ=255)                                                                                                  tGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttctt                                    >  1:200803/1‑139 (MQ=255)                                                                                                  tGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttctt                                    >  4:163280/1‑139 (MQ=255)                                                                                                  tGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttctt                                    >  7:118867/1‑139 (MQ=255)                                                                                                  tGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttctt                                    >  8:273738/1‑139 (MQ=255)                                                                                                  tGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttctt                                    >  6:312356/1‑139 (MQ=255)                                                                                                  tGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttctt                                    >  5:48393/1‑139 (MQ=255)                                                                                                  tGCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACACTTTTTTCTTTTGTCTTGTAAAAGTATtttctt                                    >  3:197757/1‑139 (MQ=255)                                                                                                  tGCTTGATATGATGAATTGTCAATATCTATGTATAAACAATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttctt                                    >  5:87035/1‑139 (MQ=255)                                                                                                   gCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttcttt                                   >  3:91419/1‑139 (MQ=255)                                                                                                   gCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttcttt                                   >  1:6769/1‑139 (MQ=255)                                                                                                   gCTTGATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttcttt                                   >  2:145665/1‑139 (MQ=255)                                                                                                       gATATGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTctttcttt                               >  6:228424/1‑139 (MQ=255)                                                                                                        atatGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTaaa                                               <  7:234445/122‑1 (MQ=255)                                                                                                        atatGATGAATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTaaa                                               >  8:234445/1‑122 (MQ=255)                                                                                                             atgaATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAAGAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGtaag                          >  7:173773/1‑138 (MQ=255)                                                                                                              tgaATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGgaagaa                        <  8:112363/139‑1 (MQ=255)                                                                                                              tgaATTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGgaagaa                        <  8:178198/139‑1 (MQ=255)                                                                                                                aaTTGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGaaa                       <  2:7682/138‑1 (MQ=255)                                                                                                                  ttGTCAATATGTATGTATAAACTATACAACTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGc                    <  2:219031/139‑1 (MQ=255)                                                                                                                   tGTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCt                   >  6:68825/1‑139 (MQ=255)                                                                                                                    gTCAATATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCt                   >  8:39996/1‑138 (MQ=255)                                                                                                                       aaTATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttct                                     >  6:198744/1‑117 (MQ=255)                                                                                                                       aaTATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATtttct                                     <  5:198744/117‑1 (MQ=255)                                                                                                                       aaTATCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCttat                <  8:118867/138‑1 (MQ=255)                                                                                                                        atatCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCttatta              <  5:135637/139‑1 (MQ=255)                                                                                                                        atatCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCttatta              <  4:197757/139‑1 (MQ=255)                                                                                                                        atatCTATGTATAAACAATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCttatta              <  6:87035/139‑1 (MQ=255)                                                                                                                           tCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTaca            <  5:312356/138‑1 (MQ=255)                                                                                                                           tCTATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTaca            <  3:95310/138‑1 (MQ=255)                                                                                                                             tATGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTAcaca          >  1:186918/1‑138 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAATAAATGCTTATTACACata        >  4:117992/1‑138 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCttatta              >  1:246789/1‑132 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCttatta              <  2:246789/132‑1 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTac             >  6:282041/1‑133 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTac             <  5:282041/133‑1 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTACACata        >  7:69468/1‑138 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTACACata        >  3:82764/1‑138 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTACACata        >  1:302855/1‑138 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTACACata        >  2:297823/1‑138 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTACACata        >  3:262634/1‑138 (MQ=255)                                                                                                                               tGTATAAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTA‑TACACatat       >  6:91300/1‑138 (MQ=255)                                                                                                                                   taAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCttatta              >  4:15880/1‑128 (MQ=255)                                                                                                                                   taAACTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCttatta              <  3:15880/128‑1 (MQ=255)                                                                                                                                    aaaCTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTACACATATAATAc  >  2:49551/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        GTTCAAAAAAAGAAAGGCTATTATATCATAAAAGCAAAAAAAGTTTGACTCGGTATTTTAACTATGTTAATATTGTAAAATGCCAATGTATATATTTTGCTTGATATGATGAATTGTCAATATCTATGTATAAATTATACAAGTATGGAAATGCTAATAAAATCGGTATTTGTTTTTGGATGTGGTTTTCTTAATTAGAAACCCTTTTTTCTTTTGTCTTGTAAAAGTATTTTCTTTCTTTGGAAGAAATGCTTATTACACATATAATAC  >  BS168_reference_genome/121626‑121895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑