Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	529,425	G→A	intergenic (+3/+80)	trnS‑Leu2 → / ← ydcL	tRNA‑Leu/ICEBs1 mobile element: integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	529,425	0	G	A	100.0%	28.6 / NA	15	intergenic (+3/+80)	trnS‑Leu2/ydcL	tRNA‑Leu/ICEBs1 mobile element: integrase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/5);  total (10/5)

TACTAAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑A‑‑TCAATGATACCAAGGGTTTTGACACCCTTGGTATTTTTTTG  >  BS168_reference_genome/529303‑529460                                                                                                                               |                                    tactaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGttt                        >  6:106265/1‑138 (MQ=255)  actaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGTCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCTGCCGC‑‑ATCTCAATAATAACAAAGGttta                       >  2:148305/1‑137 (MQ=21)  actaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGtttaa                      >  4:254952/1‑137 (MQ=21)  actaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGtttaa                      >  6:308094/1‑137 (MQ=21)  actaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGGTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGtttaa                      >  6:206320/1‑137 (MQ=21)  actaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACCCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGtttaa                      >  6:261349/1‑137 (MQ=18)  actaAACCAGCACAAAACAATTTAATGATCACGCGGTCTTGGCGGAAAGGCAGACGCGCTATGTTGAGGTCCTAGTGGTTGAATAACCCGTGGAGGTTCAAGGCCTCTCGGCCGCATA‑‑TCAATAATAACAAGGGtgtaa                      >  5:41286/1‑135 (MQ=18)   ctaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGtttaac                     <  2:45621/139‑4 (MQ=18)   ctaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGtttaac                     <  3:254952/139‑4 (MQ=18)   ctaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGtttaac                     <  4:232426/139‑4 (MQ=18)   ctaAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGtttaac                     <  5:206320/139‑4 (MQ=18)            cacaacacaaTTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGTTTAACCACCTTTgg            >  1:113702/1‑139 (MQ=17)            cacaacacaaTTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGTTTAACCACCTTTgg            >  3:197774/1‑139 (MQ=17)              caacacaaTTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGTTTAACCACCTTTGGTa          <  5:261349/139‑1 (MQ=14)                      tttAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑ATCTCAATAATACCAAGGGTTTAACCACCTTTGGTAttattttt  >  7:189230/1‑138 (MQ=11)                                                                                                                               |                                    TACTAAACCAGCACAACACAATTTAATGATCACGCGGTCGTGGCGGAATGGCAGACGCGCTAGGTTGAGGGCCTAGTGGGTGAATAACCCGTGGAGGTTCAAGTCCTCTCGGCCGC‑‑A‑‑TCAATGATACCAAGGGTTTTGACACCCTTGGTATTTTTTTG  >  BS168_reference_genome/529303‑529460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑