Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	892,022	A→T	N196I (AAT→ATT)	malR →	transcriptional activator of the Mal operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	892,022	0	A	T	100.0%	232.1 / NA	64	N196I (AAT→ATT)	malR	transcriptional activator of the Mal operon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (35/29);  total (35/29)

TTTAATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCG  >  BS168_reference_genome/891889‑892156                                                                                                                                      |                                                                                                                                       tttaatttGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTa                                                                                                                                    >  1:274560/1‑138 (MQ=255)        tGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACa                                                                                                                             <  7:89917/138‑1 (MQ=255)         gATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAAt                                                                                                                           >  1:249313/1‑139 (MQ=255)               cgATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTc                                                                                                                     <  5:54234/139‑1 (MQ=255)                     ttttAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAg                                                                                                               <  6:116378/139‑1 (MQ=255)                          aaGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGAc                                                                                                          <  2:221489/139‑1 (MQ=255)                           aGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACt                                                                                                         <  1:63178/139‑1 (MQ=255)                               aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                      >  3:334350/1‑138 (MQ=255)                               aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                      >  7:16033/1‑138 (MQ=255)                               aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                      >  8:239913/1‑138 (MQ=255)                               aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                      >  1:138126/1‑138 (MQ=255)                               aaCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCg                                                                                                      >  2:180110/1‑138 (MQ=255)                                aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                    <  5:303638/139‑1 (MQ=255)                                aCGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                    <  8:16033/139‑1 (MQ=255)                                    tCTGTTTATCATCATTTCTCTCTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAAt                                                                                                >  4:139735/1‑139 (MQ=255)                                       gTTTATCATCATTTCTCTTTCCGGTGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCtt                                                                                             >  8:118808/1‑139 (MQ=255)                                              atcatTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCg                                                                                       <  4:23476/138‑1 (MQ=255)                                              atcatTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGa                                                                                      >  8:181427/1‑139 (MQ=255)                                                  tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg                                                                                  >  8:277220/1‑139 (MQ=255)                                                  tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg                                                                                  <  2:329700/139‑1 (MQ=255)                                                  tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg                                                                                  >  5:190800/1‑139 (MQ=255)                                                  tttCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAg                                                                                  >  2:53468/1‑139 (MQ=255)                                                     ctctTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCa                                                                                <  7:181427/138‑1 (MQ=255)                                                          tCCGGAGAAACACCGGGGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtg                                                                          >  4:199344/1‑139 (MQ=255)                                                          tCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAAc                                                                             <  1:53468/136‑1 (MQ=255)                                                          tCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtg                                                                          >  3:282837/1‑139 (MQ=255)                                                           ccGGAGAAACACCGGGGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtg                                                                          <  3:199344/138‑1 (MQ=255)                                                            cGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtgt                                                                         <  2:138126/138‑1 (MQ=255)                                                                    cacCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCATTTATTTACATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAg                                                                <  5:274620/139‑1 (MQ=255)                                                                         gAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGa                                                             >  2:68123/1‑137 (MQ=255)                                                                                    tCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                    >  1:258222/1‑87 (MQ=255)                                                                                    tCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCa                                                                                                    <  2:258222/87‑1 (MQ=255)                                                                                       ggCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTc                                             <  7:239913/139‑1 (MQ=255)                                                                                                    tCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACa                                                                     >  8:304562/1‑102 (MQ=255)                                                                                                    tCTGCAAAGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACa                                                                     <  7:304562/102‑1 (MQ=255)                                                                                                           aGGGTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTTGCTTTTGCACCGTTTTTtct                         >  5:273949/1‑139 (MQ=255)                                                                                                             ggTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCg                       <  6:190800/139‑1 (MQ=255)                                                                                                             ggTATTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCCCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCg                       <  3:139735/139‑1 (MQ=255)                                                                                                                aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAt                                                                                                   <  7:232728/60‑1 (MQ=255)                                                                                                                aTTCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCAt                                                                                                   >  8:232728/1‑60 (MQ=255)                                                                                                                  tCCCTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGc                  >  7:45729/1‑139 (MQ=255)                                                                                                                    ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATTGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                 <  4:334350/138‑1 (MQ=255)                                                                                                                    ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                 <  8:45729/138‑1 (MQ=255)                                                                                                                    ccTTTATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCg                 <  3:173114/138‑1 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCACACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCGTTTGGACCCTTTTTGAGGGTTGGCGCAGc             >  1:170270/1‑139 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtg                                                                          >  5:121662/1‑78 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCtg                                                                          <  6:121662/78‑1 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCa                                    >  6:73904/1‑116 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCa                                    <  5:73904/116‑1 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg              >  6:337905/1‑138 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg              >  8:115019/1‑138 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg              >  7:17286/1‑138 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg              >  2:29141/1‑138 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg              >  8:298938/1‑138 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg              >  1:2724/1‑138 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTGTTTCTCGTTGGCGAAg              >  4:46464/1‑138 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACAGTTTTTTCTCGTTGGCGAAg              >  5:229346/1‑138 (MQ=255)                                                                                                                       ttATTTCCATTACGATTTAAAAAAACAATGTCCTCGCCCAGCTCACTCCGCATAATCTTTATGCGACAAGCAACCCTGTCACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAg              >  2:44200/1‑138 (MQ=255)                                                                                                                              cATTACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTc      <  7:277220/139‑1 (MQ=255)                                                                                                                                ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGt                      <  5:54919/121‑1 (MQ=255)                                                                                                                                ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGt                      >  6:54919/1‑121 (MQ=255)                                                                                                                                ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcg    <  8:17286/139‑1 (MQ=255)                                                                                                                                ttACGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTATCGGTGGCGAAGCATTGTTCcg    >  5:27351/1‑139 (MQ=255)                                                                                                                                   cGATTTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCcgcg  <  7:118808/138‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TTTAATTTGATGCGCGATGATTTTAAAGTGAACGATCTGTTTATCATCATTTCTCTTTCCGGAGAAACACCGGAGCTGATTCCTCAGGCAAGAATGCTTTCTGCAAAGGGTATTCCCTTTATTTCCATTACGAATTTAAAAAACAATGTCCTCGCCCAGCTGACTCCGCATAATCTTTATGCGACAAGCAAACCTGTGACATTAAGCGATAGAACGGAAATTGTCGCTTTTGCACCGTTTTTTCTCGTTGGCGAAGCATTGTTCCGCG  >  BS168_reference_genome/891889‑892156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑