BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,039	C→T	intergenic (–/‑939)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,039	0	C	T	99.8%	57775.0 / NA	16501	intergenic (–/‑939)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (24/7); new base T (7460/8962); total (7510/8991)
Fisher's exact test for biased strand distribution p-value = 4.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 16838 total aligned reads displayed.

ACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTAT  >  pSIJ663_pHT315_araE_xy/1892‑2188

ccAAATAGCCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTAt                                                                                                                                                     <  2:4360540/149‑1 (MQ=255)

  acatttccGCTTGGGTCCATCTTTATGGCAACTAGCATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGCTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaa                                                                                                                                                   <  2:1411817/144‑1 (MQ=255)

  aaaTAGCCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaa                                                                                                                                                   <  2:2714213/150‑1 (MQ=255)

  aaaTAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaa                                                                                                                                                   >  2:1237834/1‑150 (MQ=255)

   aaTAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATaaa                                                                                                                                                  <  2:1760698/150‑1 (MQ=255)

    aTAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAAt                                                                                                                                                 <  2:2268855/150‑1 (MQ=255)

       aCCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATAcc                                                                                                                                              >  1:4351277/1‑150 (MQ=255)

         cGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAg                                                                                                                                            >  2:1064866/1‑150 (MQ=255)

          ccTTGGGTCCAACTTTATAGCAATTAGTATATGTTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAgg                                                                                                                                           <  1:2734548/149‑1 (MQ=255)

            ttGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAt                                                                                                                                         <  1:312541/150‑1 (MQ=255)

               ggcccAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                                      <  2:3785928/147‑1 (MQ=255)

               ggTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGAtgtt                                                                                                                                      >  1:1814919/1‑150 (MQ=255)

                   cAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGttt                                                                                                                                  >  2:1496967/1‑150 (MQ=255)

                   cAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGttt                                                                                                                                  >  2:369496/1‑150 (MQ=255)

                   cAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGttt                                                                                                                                  >  2:3230627/1‑150 (MQ=255)

                    aaCTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTc                                                                                                                                 >  2:133164/1‑150 (MQ=255)

                      cTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCtt                                                                                                                               <  2:3513977/150‑1 (MQ=255)

                      cTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCtt                                                                                                                               <  2:3150287/150‑1 (MQ=255)

                        ttATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTa                                                                                                                             <  2:1583994/150‑1 (MQ=255)

                        ttATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTa                                                                                                                             >  2:609269/1‑150 (MQ=255)

                          ataGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACg                                                                                                                           <  2:2617827/150‑1 (MQ=255)

                            aGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATTGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTg                                                                                                                         >  2:2010393/1‑150 (MQ=255)

                                   aGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTtata                                                                                                                  <  1:2397383/150‑1 (MQ=255)

                                    gTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATaa                                                                                                                 <  2:842497/150‑1 (MQ=255)

                                       tatGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATc                                                                                                              <  2:1677657/150‑1 (MQ=255)

                                        atGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATcc                                                                                                             <  2:41812/150‑1 (MQ=255)

                                          ggTCATTTAAAATTTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGAGGGGGTGTCCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCgg                                                                                                           <  1:189603/150‑1 (MQ=255)

                                             cATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTAATAAAGACCAGGATGTTGTTTCTTTACGAGCTTTATAAATCCgggta                                                                                                        >  1:2145130/1‑148 (MQ=255)

                                             cATTTAAAATCTTTACCAATTCAACCCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGCGTTCAACGATATCTTTTAAGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCCTTACGTGCTCTATAAATCCGGGaa                                                                                                        >  1:823025/1‑150 (MQ=255)

                                               tttACACTCTTTACCCATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAAc                                                                                                      <  2:3424161/150‑1 (MQ=255)

                                               tttAAAATTTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGACGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAAc                                                                                                      <  2:1155299/150‑1 (MQ=255)

                                                ctAAAATCTATTCCAATTCAACGCTAGTAGGTACTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACa                                                                                                     <  1:3659222/149‑1 (MQ=255)

                                                  aaaaTCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAtt                                                                                                   >  1:2577551/1‑150 (MQ=255)

                                                   aaaTCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGGATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACAttt                                                                                                  >  1:979686/1‑150 (MQ=255)

                                                       cTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                                              <  2:4648526/150‑1 (MQ=255)

                                                       cTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTAGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTAc                                                                                              <  1:662537/150‑1 (MQ=255)

                                                         ttACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACAt                                                                                            <  1:3578148/150‑1 (MQ=255)

                                                           aCCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTTTAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCg                                                                                          >  2:2431401/1‑150 (MQ=255)

                                                              aaTTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTa                                                                                       >  1:3916987/1‑150 (MQ=255)

                                                               aTTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAg                                                                                      <  1:1075035/150‑1 (MQ=255)

                                                               aTTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAg                                                                                      <  2:2096778/150‑1 (MQ=255)

                                                                ttCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGa                                                                                     <  2:289316/150‑1 (MQ=255)

                                                                     cGCTATTAGGTTCTTTAGGATTTTGCCCGACTTAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGc                                                                                >  1:1239655/1‑150 (MQ=255)

                                                                      gCTATTAGGTTCTTTAGGATTGTGCCCGACATAGTCGGGGCGCTCAACGATACCTCTCATGTGCGATGAATATGTTTTATACATACCAGGATGTCGTTTCTTTACGTGCTCTATAAGTCCCGGAAACATTTTTACATCGTTAGAAGTGca                                                                               >  2:2978338/1‑150 (MQ=255)

                                                                       cTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaa                                                                              >  1:1976284/1‑150 (MQ=255)

                                                                         aTTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagt                                                                            <  2:4754442/150‑1 (MQ=255)

                                                                              gTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaagt                                                                       >  1:4260372/1‑150 (MQ=255)

                                                                              gTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaagt                                                                       <  1:2971817/150‑1 (MQ=255)

                                                                               gtCTTTAGGATCTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTCCGTGCTATATACATCCGGGAAACATTTTTACATCGTTAGAAGTGcaagtcaagat                                                                      <  1:1498645/149‑3 (MQ=255)

                                                                                  tttAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTtata                                                                   >  2:1847894/1‑150 (MQ=255)

                                                                                    tAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGGTATATg                                                                 >  1:1161751/1‑150 (MQ=255)

                                                                                       gATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTGAGAAGTGCAAGTCAAGTTAGATGTAt                                                              >  1:4720448/1‑150 (MQ=255)

                                                                                         ttttGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCt                                                            <  2:2809172/150‑1 (MQ=255)

                                                                                            tGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGCCAAGTTATATGGATCtata                                                         >  2:920790/1‑150 (MQ=255)

                                                                                              cccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGTATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTATACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAAt                                                       >  1:3828731/1‑150 (MQ=255)

                                                                                               ccGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGTTGAATATTTTTTATAAATACCAGGTTGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACCTTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATGTATAATg                                                      <  1:3384248/150‑1 (MQ=255)

                                                                                                cGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGa                                                     >  1:463694/1‑150 (MQ=255)

                                                                                                   cATAGTCGGGGTGTTCACCGATATCTTTTATGTGCGATGAATATTTGTTATTAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGCCAAGTTATATGTATCTATAATGAttt                                                  <  1:3737749/150‑1 (MQ=255)

                                                                                                   cATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAttt                                                  <  1:1328315/150‑1 (MQ=255)

                                                                                                   cATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTAAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAttt                                                  >  1:3475511/1‑150 (MQ=255)

                                                                                                   cATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGGTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGAttt                                                  >  1:906119/1‑150 (MQ=255)

                                                                                                     tAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgt                                                <  1:1736461/150‑1 (MQ=255)

                                                                                                      aGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTGTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTtgtg                                               >  1:1000204/1‑150 (MQ=255)

                                                                                                         cGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGaa                                            <  1:1408726/150‑1 (MQ=255)

                                                                                                         cGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGGTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGaa                                            <  2:67983/150‑1 (MQ=255)

                                                                                                           gggTGTTCAACGATATCTTTTATGTGGGATGAATATTTTTTATAAATACCAGGATGTTGCTTCTTTCCGTGCTTTATAAATCCGGGAAACAATTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGt                                          <  1:2660845/150‑1 (MQ=255)

                                                                                                            ggTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGtt                                         >  2:3603832/1‑150 (MQ=255)

                                                                                                             gtgtGCATCGATATCTTTTATGTGCGATGAATATTTTTTATAAATCCCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGttt                                        <  1:3209235/150‑1 (MQ=255)

                                                                                                              tgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGtttt                                       >  1:2805227/1‑150 (MQ=255)

                                                                                                              tgtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGtttt                                       <  2:4070142/150‑1 (MQ=255)

                                                                                                               gtTCATCGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTg                                      <  1:3041296/150‑1 (MQ=255)

                                                                                                               gtTCAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTg                                      <  1:2307950/150‑1 (MQ=255)

                                                                                                                  cAACGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCca                                   >  2:1926931/1‑150 (MQ=255)

                                                                                                                    aCGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAACCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTATGCcaca                                 <  1:2218222/150‑1 (MQ=255)

                                                                                                                     cGATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaa                                >  2:3331862/1‑150 (MQ=255)

                                                                                                                      gATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaac                               >  2:4565438/1‑150 (MQ=255)

                                                                                                                      gATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaac                               <  1:4009522/150‑1 (MQ=255)

                                                                                                                      gATATCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaac                               >  2:3982877/1‑150 (MQ=255)

                                                                                                                       atatCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCacaaca                              <  1:2888602/150‑1 (MQ=255)

                                                                                                                         atCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGt                            <  1:1585637/150‑1 (MQ=255)

                                                                                                                          tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt                           <  1:329884/150‑1 (MQ=255)

                                                                                                                          tCTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGtt                           <  1:2482487/150‑1 (MQ=255)

                                                                                                                           cTTTTATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAACCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTg                          <  1:4185036/150‑1 (MQ=255)

                                                                                                                             tttATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTggg                        >  1:4444935/1‑149 (MQ=255)

                                                                                                                               tATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGttt                      >  1:166114/1‑150 (MQ=255)

                                                                                                                               tATGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGGGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGGTTTGCCACAACAGTTGGttt                      >  2:2353615/1‑150 (MQ=255)

                                                                                                                                aTGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGttta                     >  2:2530622/1‑150 (MQ=255)

                                                                                                                                aTGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGttta                     <  1:579706/150‑1 (MQ=255)

                                                                                                                                aTGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGGTGGttta                     >  2:3070901/1‑150 (MQ=255)

                                                                                                                                aTGTGCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGATATATGTATCTATAATGATTTGTGGAAGTGTTGCCACAACAGTTGGttta                     >  2:4272369/1‑150 (MQ=255)

                                                                                                                                 tgtgCGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGtttat                    >  1:2052801/1‑150 (MQ=255)

                                                                                                                                    gCGATGAATATTTTTTTTAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGtttattta                 >  1:735664/1‑150 (MQ=255)

                                                                                                                                     cGATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTAc                <  1:505950/150‑1 (MQ=255)

                                                                                                                                      gATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACa               >  2:535510/1‑150 (MQ=255)

                                                                                                                                      gATGAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACa               >  2:755130/1‑150 (MQ=255)

                                                                                                                                         gAATATTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATc            <  1:4098800/150‑1 (MQ=255)

                                                                                                                                           atatTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCtt          >  2:2180957/1‑150 (MQ=255)

                                                                                                                                           atatTTTTTATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCtt          <  1:4530554/150‑1 (MQ=255)

                                                                                                                                             tttttttATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCtttt        >  2:388348/2‑150 (MQ=255)

                                                                                                                                               ttttTATAAATACCAGGAGGTTGTTTCTTTACGGGCTTAATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGTTTCGTGGAAGTCTTGCCACAACAGTTCGTTTATTTACAATCtttttt      >  2:4465703/1‑150 (MQ=255)

ACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTAT  >  pSIJ663_pHT315_araE_xy/1892‑2188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`