BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,049	C→T	intergenic (+318/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,049	0	C	T	96.9%	48726.0 / NA	15912	intergenic (+318/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base C (30/456); new base T (9803/5513); total (9894/6018)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 16162 total aligned reads displayed.

TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGA  >  pSIJ663_pHT315_araE_xy/4902‑5198

 tGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGGGCGCAGGTACCAAATACTGTTCtt                                                                                                                                                    >  2:3511731/1‑150 (MQ=255)

 tGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCtt                                                                                                                                                    <  2:4651222/150‑1 (MQ=255)

 tGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCtt                                                                                                                                                    <  2:3229011/150‑1 (MQ=255)

     aTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAg                                                                                                                                                >  2:926005/1‑150 (MQ=255)

     aTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAg                                                                                                                                                >  2:1230390/1‑150 (MQ=255)

      tCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTATCTGGCTTCAGCAGAGCGCAGAGACCAAATACTGTTCTTCTTgt                                                                                                                                               >  1:1074516/1‑150 (MQ=255)

          ttttttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAg                                                                                                                                           >  1:2536097/1‑150 (MQ=255)

           tttttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGGGCGCAGGTACCAAATACTGTTCTTCTAGTGTAGc                                                                                                                                          >  2:3605363/1‑150 (MQ=255)

            ttttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGGGGCTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGACACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                                         >  1:2779540/1‑150 (MQ=255)

            ttttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAACACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGcc                                                                                                                                         >  1:890832/1‑150 (MQ=255)

                cTGCGCGTACTCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAg                                                                                                                                     <  2:2090775/150‑1 (MQ=255)

                cTGCGCGTAATCTGCTGCTTGCAAACAAAAAACCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAg                                                                                                                                     <  2:2812777/150‑1 (MQ=255)

                cTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAg                                                                                                                                     <  2:236187/150‑1 (MQ=255)

                  gcgcGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGGGCTACCAACTCTTTTTCCGAAGGGAACTGGCTTCAGCAGAGCGCAGATACCCAATACTGTTCTTCTAGTGTAGCCGTAGtt                                                                                                                                   >  1:3440195/1‑150 (MQ=255)

                      gTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGGTCTTCTAGTGTAGCCGTAGTTAGGc                                                                                                                               >  2:1557093/1‑150 (MQ=255)

                       tAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGGAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcc                                                                                                                              >  2:4146060/1‑150 (MQ=255)

                         aTCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac                                                                                                                            <  1:3363842/150‑1 (MQ=255)

                           ctcctgctTGCAAACAAAAAAACCACCGCTACCGGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccacca                                                                                                                          <  1:3950635/147‑1 (MQ=255)

                             gctgctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACt                                                                                                                        >  1:4450251/1‑150 (MQ=255)

                              ctgctTGCAAACAAAAAAACCACCGCTACCAGGGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACtt                                                                                                                       <  1:1784939/150‑1 (MQ=255)

                               tgctTGCAAACAAAAAAACCACCGCTCCCATCGGTGGTTTGTTGGCCGGATCAAGAGCTACCAACTCTTTTTCCGAGGGTAACTGGCTCCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc                                                                                                                      <  2:2194961/150‑1 (MQ=255)

                                gctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCa                                                                                                                     >  1:805196/1‑150 (MQ=255)

                                 tttGCAACCAAAAAAACCCCCGCTACCGGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCaa                                                                                                                    <  1:4120361/149‑1 (MQ=255)

                                  ttGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAg                                                                                                                   >  1:2947741/1‑150 (MQ=255)

                                   tGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGATCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGa                                                                                                                  >  1:361519/1‑150 (MQ=255)

                                     caaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAc                                                                                                                >  2:42264/1‑150 (MQ=255)

                                     caaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAc                                                                                                                >  2:4559918/1‑150 (MQ=255)

                                      aaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTGCAGCGGAGCGCAGATACCAAAGACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCACGGAct                                                                                                               >  1:3871128/1‑150 (MQ=255)

                                        ccaaaAAACCCACCCCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAActct                                                                                                             <  2:633363/149‑1 (MQ=255)

                                        acaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGGGTAGCCGTAGTTAGGCCACCACTTCAAGAActct                                                                                                             >  1:1000348/1‑150 (MQ=255)

                                            aaaccccaccGCTACCAGCGGTGGTTTGTTTGCCGGACCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                                         <  2:200699/145‑1 (MQ=255)

                                            aaaacCCCCCGCTACCAGCGGTGGTTTGTTTGCCGGACCAAGAGCTACCATCTCTTTTTCCGAAGGTAACTGCCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCAAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                                         <  2:2624900/150‑1 (MQ=255)

                                            aaaaaCCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGGGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg                                                                                                         >  1:4035273/1‑150 (MQ=255)

                                             caacccaccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTTTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATTCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGc                                                                                                        <  2:4474471/146‑1 (MQ=255)

                                              accccaccGCTCCCTGCGGTTGTTTGTTTGCCGGAACAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTCGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCa                                                                                                       <  1:154280/147‑1 (MQ=255)

                                                accaccGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGGGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGGAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTGGCAcc                                                                                                     >  1:1385971/1‑150 (MQ=255)

                                                   accGCTAGCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGcc                                                                                                  <  1:627167/150‑1 (MQ=255)

                                                        tcccggCGTTGGTTTGTTTGCCGGATCAAGCGTTACCACCTCTTTTGCCGAAGTAACCTGGCTTCAGCAGAGCGCAGTTACCAAATCCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAt                                                                                             <  1:2463755/145‑1 (MQ=255)

                                                        tACCAGCGGTGGTTTGTTTGCCGGGTCAGGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCGGATACCAAATACTGTTCTTCTAGGGAAGCCGTAGTTAGGCCACCACATCAAGAACTCTGGAGCACCGccaaaat                                                                                             >  1:2210617/1‑145 (MQ=255)

                                                        tACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACAt                                                                                             >  1:1460882/1‑150 (MQ=255)

                                                           cAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCtacctacc                                                                                          >  2:3133295/1‑150 (MQ=255)

                                                              gggtggtTTGTTTGCCGAGTCAAGAGCTACCAACCGATTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGTTGCCAAATACTTTTCTCCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCg                                                                                       <  1:1221786/149‑1 (MQ=255)

                                                               ggtggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATCCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGc                                                                                      >  2:4313256/1‑150 (MQ=255)

                                                                gtggtTTGTTTGCCGGATCAAGAGCTACCAACTCATTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACCTCAAGAACTCTGTAGCACCGCCTACATACCTCGct                                                                                     >  1:3697306/1‑150 (MQ=255)

                                                                gtggtTTGTTTCCCGGATCAAGAGCTCCCAACCCTTTTCCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATCCCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGct                                                                                     <  2:2271493/150‑1 (MQ=255)

                                                                  ggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGctct                                                                                   >  2:52887/1‑150 (MQ=255)

                                                                   gtttgtttGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGGAGCCGAAGTTAGGCCACCACTTCAAGAACTCTGGAGCACCGCCTACATACCTCGCTCTg                                                                                  >  2:744253/1‑150 (MQ=255)

                                                                    tttgtttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACAGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGAAGTTAGGCCACCACTTCAAGAACTCTGCAACACCGCCTACATACCTCGCTCAGc                                                                                 >  1:4610867/1‑150 (MQ=255)

                                                                     ttgtttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCt                                                                                >  1:4776640/1‑150 (MQ=255)

                                                                     ttgtttgCCGGACCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCt                                                                                <  2:3325715/150‑1 (MQ=255)

                                                                          tgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATcc                                                                           >  1:1706598/1‑150 (MQ=255)

                                                                                aTCGAGACCTCCCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAc                                                                     <  1:1868196/150‑1 (MQ=255)

                                                                                 tCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTAcc                                                                    >  2:4736584/1‑150 (MQ=255)

                                                                                       gCTCCCACCCCTTTTCCCGAAGGTAACCGGCTTCAGCAGGGCGCAGACACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGgc                                                              <  1:4695556/150‑1 (MQ=255)

                                                                                           ccAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGAGgctgct                                                          >  2:2910355/1‑150 (MQ=255)

                                                                                              aCTCTTTTTCCGAGGGCAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTGCTAGTGTAGCCGCAGTCAGGCCACCACTTCCAGAACTCTGTAGCACCGCCCACATACCTCGCTCTGCTAATCCTGTTACCAGTGGGCGCTGcc                                                       >  1:2868854/1‑150 (MQ=255)

                                                                                              aCTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGcc                                                       >  2:341279/1‑150 (MQ=255)

                                                                                               ctctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCa                                                      >  1:303033/1‑150 (MQ=255)

                                                                                               ctctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCa                                                      >  2:147444/1‑150 (MQ=255)

                                                                                               ctctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCa                                                      >  2:1648326/1‑150 (MQ=255)

                                                                                               ctctTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCa                                                      >  1:203088/1‑150 (MQ=255)

                                                                                               ctctTTTTCCGAAGGAAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGGCGTAGTTAGGCCACCACTTCAAGAACTCTGGAGCACCGCCTACATACCTCGCTCTGCTAATCCTGCTACCAGGGGCTGCTGCCa                                                      >  1:2123669/1‑150 (MQ=255)

                                                                                                 ctTTTTCCGAAGGTAACTGGCTCCAGCAGAGCGCAGATACCAATTCCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGt                                                    <  2:3887996/150‑1 (MQ=255)

                                                                                                   ttttCCGAAGGTACCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTgg                                                  <  2:1394106/150‑1 (MQ=255)

                                                                                                   ttttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTgg                                                  >  1:1956053/1‑150 (MQ=255)

                                                                                                    tttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGc                                                 >  1:1144358/1‑150 (MQ=255)

                                                                                                     ttCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGGTACCAGTGGCTGCTGCCAGTGGCg                                                >  1:4378161/1‑150 (MQ=255)

                                                                                                      tCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGa                                               >  2:3016678/1‑150 (MQ=255)

                                                                                                         gAAGGTACCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATaa                                            <  2:2444627/150‑1 (MQ=255)

                                                                                                           aGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGt                                          >  2:3796141/1‑150 (MQ=255)

                                                                                                            ggTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTc                                         >  2:1920346/1‑150 (MQ=255)

                                                                                                              tAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgt                                       >  1:3202161/1‑150 (MQ=255)

                                                                                                              tAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCgt                                       >  2:3978473/1‑150 (MQ=255)

                                                                                                              tAACTGGCTTCAGCAAAGCGCAGGTACCAAATACTGTACTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTTCATACCTCGCTCTGCTTATCCTGGTACCAGTGGCCGCTGCCAGGGGCGATTAGGCgt                                       >  1:4296248/1‑150 (MQ=255)

                                                                                                                aCTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGGTAAGTCgtgt                                     >  2:3695384/1‑150 (MQ=255)

                                                                                                                       tCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCg                              >  2:838753/1‑150 (MQ=255)

                                                                                                                        cagcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCgg                             <  2:17492/150‑1 (MQ=255)

                                                                                                                         agcagAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGGTAAGTCGGGTCTTACCggg                            >  2:4396539/1‑150 (MQ=255)

                                                                                                                            agagCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTg                         >  1:3785341/1‑150 (MQ=255)

                                                                                                                            agagCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGGCGTGTCTTACCGGGTTg                         >  1:1617595/1‑150 (MQ=255)

                                                                                                                              agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGa                       >  1:4537409/1‑150 (MQ=255)

                                                                                                                              agCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGa                       >  2:1056004/1‑150 (MQ=255)

                                                                                                                               gcgcAGATACCAATTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGAc                      <  1:2619299/150‑1 (MQ=255)

                                                                                                                                cgcAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCCTGGCTTACCGGGTTGGACt                     >  1:3109601/1‑150 (MQ=255)

                                                                                                                                  cAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCa                   <  1:546687/150‑1 (MQ=255)

                                                                                                                                      tcccAAATACTTTCCTTCTGGGGTAGCCGTTGTTCGGCCACCCCTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAc               <  2:183645/148‑1 (MQ=255)

                                                                                                                                      tcccAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTGGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAc               <  1:1303395/148‑1 (MQ=255)

                                                                                                                                        ccAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGa             >  1:3520319/1‑150 (MQ=255)

                                                                                                                                         cAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTTGACTCAAGACGAt            <  2:253360/150‑1 (MQ=255)

                                                                                                                                          aaaTACTGTTCTTCTAGTGTGGCGGTAGTTAGCCCACCCCTCCAAGAACTCTGTAGCCCCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATa           <  1:2387103/150‑1 (MQ=255)

                                                                                                                                          aaaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATa           <  1:4208259/150‑1 (MQ=255)

                                                                                                                                           aaTACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGGTAGGTCGTGTCTTACCGGGGTGGACTCAAGGCGATAg          >  2:4061636/1‑150 (MQ=255)

                                                                                                                                             tcgtGTTCTTCTAGGGTAGCCGTAGTTAGGCCCCCACTTCCAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGtt        <  2:2705906/147‑1 (MQ=255)

                                                                                                                                             tACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGtt        >  1:2695375/1‑150 (MQ=255)

                                                                                                                                              aCTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGGGTCTTTCCGGGGTGGACTCAAGACGATAGTTa       >  1:711618/1‑150 (MQ=255)

                                                                                                                                               cTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGGCTCAAGACGATAGTTAc      >  2:131060/1‑150 (MQ=255)

                                                                                                                                                tGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGATCTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAcc     >  1:962996/1‑150 (MQ=255)

                                                                                                                                                tGTTCTTCTAGTGGAGCCGTGGTTAGGCCACCACTCCAAGAACTCTGTAGCTCCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAcc     <  2:1140118/150‑1 (MQ=255)

                                                                                                                                                tGTTCTTCTACTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGGGTCTTACCGGGTTGGACTCAAGACGATAGTTAcc     >  1:3280588/1‑150 (MQ=255)

                                                                                                                                                tGTCCTTCTTGTATAGCCATGGTCAGGCCACCACTTCAAGAACCCTGTAGCCCCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCCTACCGGGTTGGACTCAAGACGATAGTTAcc     <  1:2302071/150‑1 (MQ=255)

TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGA  >  pSIJ663_pHT315_araE_xy/4902‑5198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`