Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,344 C→T S53F (TCT→TTT)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,3440CT99.8% 1530.8 / NA 433S53F (TCT→TTT) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (258/173);  total (259/174)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 439 total aligned reads displayed.

AAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGC  >  CP006881/3723197‑3723490
                                                                                                                                                   |                                                                                                                                                  
aaGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTa                                                                                                                                                  >  1:2547709/1‑150 (MQ=255)
 aGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTAt                                                                                                                                                 >  1:872451/1‑150 (MQ=255)
   cATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATgg                                                                                                                                               >  1:3230827/1‑150 (MQ=255)
   cATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATgg                                                                                                                                               <  2:2022190/150‑1 (MQ=255)
    aTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGc                                                                                                                                              <  2:1105666/150‑1 (MQ=255)
     ttacAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGct                                                                                                                                             >  2:4429739/4‑149 (MQ=255)
      tacAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGGAGCTATTTCTCTATGGctg                                                                                                                                            <  1:3080878/148‑3 (MQ=255)
      tacAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGctg                                                                                                                                            >  2:1623427/3‑148 (MQ=255)
       acAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGa                                                                                                                                           >  2:958454/2‑150 (MQ=255)
        cAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGAt                                                                                                                                          >  2:682226/1‑150 (MQ=255)
         aaaagGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGctggtt                                                                                                                                         >  2:38275/1‑145 (MQ=255)
         aaaagGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGAtt                                                                                                                                         >  2:3600413/1‑150 (MQ=255)
          aaagGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATtc                                                                                                                                        <  2:2258623/150‑1 (MQ=255)
           aaggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATtct                                                                                                                                       <  1:918669/147‑1 (MQ=255)
           aaggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATtct                                                                                                                                       >  2:2854724/4‑150 (MQ=255)
             ggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTcc                                                                                                                                     >  1:4054131/2‑150 (MQ=255)
             ggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTcc                                                                                                                                     >  1:135518/2‑150 (MQ=255)
             ggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTcc                                                                                                                                     >  2:2637501/2‑150 (MQ=255)
             ggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTcc                                                                                                                                     >  2:3685415/2‑150 (MQ=255)
              gTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCA‑TTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGt                                                                                                                                   <  1:2021533/150‑1 (MQ=255)
               ttGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGt                                                                                                                                   >  1:3681532/1‑150 (MQ=255)
                 gAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGGTGATTCTCCGttt                                                                                                                                 >  2:4072676/1‑150 (MQ=255)
                   aCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGGTTCTCCGTTTGc                                                                                                                               >  2:2207545/1‑150 (MQ=255)
                      gTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCctt                                                                                                                            >  1:2696813/1‑150 (MQ=255)
                          gCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCg                                                                                                                        <  2:4110488/150‑1 (MQ=255)
                            tttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGc                                                                                                                      >  2:4209204/1‑150 (MQ=255)
                             ttggaaaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCATAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCa                                                                                                                     >  2:3527293/7‑150 (MQ=255)
                             ttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCa                                                                                                                     >  2:628588/1‑150 (MQ=255)
                             ttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCa                                                                                                                     >  2:1210958/1‑150 (MQ=255)
                             ttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCCGCa                                                                                                                     >  1:3354552/1‑150 (MQ=255)
                             ttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGCCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCa                                                                                                                     >  2:149492/1‑150 (MQ=255)
                                 aaaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCg                                                                                                                 >  2:909915/3‑150 (MQ=255)
                                 aaaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCg                                                                                                                 <  2:3175370/148‑1 (MQ=255)
                                  aaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGGTTCTCCGATTGCCCTTCCGGCAGCCGc                                                                                                                >  2:2070316/2‑150 (MQ=255)
                                   aaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCt                                                                                                               <  2:1830944/150‑1 (MQ=255)
                                   aaacaaaacaTATCCGCTTCAAGCAAAATCCGTTGTGTGCTCCGGTCCATTTTGGCCAGTTAATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCt                                                                                                               <  1:2091760/150‑1 (MQ=255)
                                       aaaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATATTGGCCAGTATATTTATTGGTTTTGGAATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCGGCCGCTGTca                                                                                                           >  2:2400498/2‑150 (MQ=255)
                                        aaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcac                                                                                                          >  2:3446155/1‑150 (MQ=255)
                                        aaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGACATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcac                                                                                                          >  2:3908197/1‑150 (MQ=255)
                                         aaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGAATCTTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTcact                                                                                                         <  1:4378480/150‑2 (MQ=255)
                                           cATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACttt                                                                                                       >  2:4850/1‑150 (MQ=255)
                                             tatTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATCTTGGCCAGTATATTTATTGGTGTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCGGATTCTTCGTTTGCCTTTCCGGCTGCCGCTGGCACTTTCg                                                                                                     >  2:2717953/1‑150 (MQ=255)
                                                        aGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGGTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTAtt                                                                                          <  1:507642/150‑1 (MQ=255)
                                                           aaaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctg                                                                                       >  2:4593780/1‑150 (MQ=255)
                                                            aaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCCGTCACTTTCGGGGCCGCGATTctga                                                                                      >  1:1250235/1‑149 (MQ=255)
                                                             aaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctgat                                                                                     >  2:4285368/1‑148 (MQ=255)
                                                              aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctgatg                                                                                    >  2:2975731/1‑150 (MQ=255)
                                                                 cGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTTTTTCTTTATGGCTGATTCTCCGTTTGCCTTCCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGAtt                                                                                 <  2:3633962/150‑1 (MQ=255)
                                                                 cGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGAtt                                                                                 >  1:2486908/1‑150 (MQ=255)
                                                                 cGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGAtt                                                                                 <  1:1008748/150‑1 (MQ=255)
                                                                 cGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCCATTCTGATGAtt                                                                                 >  1:3984065/1‑150 (MQ=255)
                                                                    tATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCt                                                                              <  1:3755392/150‑1 (MQ=255)
                                                                     aTGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCACCTATTCCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCtt                                                                             <  1:1576929/150‑1 (MQ=255)
                                                                          cttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTCTTCTGATGGTTGCTTACGGa                                                                        >  2:4693134/7‑150 (MQ=255)
                                                                              ggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGgggggg                                                                    >  2:1582892/3‑148 (MQ=255)
                                                                              ggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGgggggg                                                                    >  2:2329168/3‑148 (MQ=255)
                                                                               gtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCgg                                                                   >  1:1670799/2‑150 (MQ=255)
                                                                                  cATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGgaga                                                                >  2:86459/1‑150 (MQ=255)
                                                                                    ttttGGCCAGTATATTTTTTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGatt                                                              <  1:2425287/150‑1 (MQ=255)
                                                                                     tttGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCGTACGGAGGCGGAGattt                                                             >  1:4467336/1‑150 (MQ=255)
                                                                                       tGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCGATTCTGATGATTACTTACGGAGGCGGAGatttat                                                           >  1:605332/1‑150 (MQ=255)
                                                                                        ggCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGatttatt                                                          <  1:39181/150‑1 (MQ=255)
                                                                                          ccAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGAtttattta                                                        <  2:1258752/150‑1 (MQ=255)
                                                                                           cAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTAc                                                       <  1:4180907/150‑1 (MQ=255)
                                                                                             gtatatttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCGGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTAccg                                                     <  2:3230827/145‑3 (MQ=255)
                                                                                                tatttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCa                                                  <  2:2174815/148‑1 (MQ=255)
                                                                                                 atttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGGGGCGGAGATTTATTTACCGGCaa                                                 >  2:450566/2‑150 (MQ=255)
                                                                                                  tttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAac                                                >  1:4230431/1‑150 (MQ=255)
                                                                                                   ttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGGTTGCTTACGGAGGCGGAGATTTATTTACCGGCAaca                                               >  2:3343521/1‑150 (MQ=255)
                                                                                                   ttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAaca                                               <  1:2848812/150‑1 (MQ=255)
                                                                                                   ttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTACTTACCGGCAaca                                               >  2:2586115/1‑150 (MQ=255)
                                                                                                   ttATTGGGTTTGGCATTACAGCCGCAGGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGGTTGCTTACGGAGGCGGGGAGTTATTTACCGGCAaca                                               >  2:1913003/1‑150 (MQ=255)
                                                                                                    tATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGGGGGGAGTTAATGACCGGCAacac                                              >  1:3457199/1‑150 (MQ=255)
                                                                                                         gttttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACctttt                                         >  2:2510865/6‑150 (MQ=255)
                                                                                                          ttttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTa                                        >  1:45941/5‑150 (MQ=255)
                                                                                                           tttggCACTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTAATTTACCGGCAACACCTTTTAt                                       <  1:2243362/147‑1 (MQ=255)
                                                                                                             tggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATACTGATGATTGCTTACGGGGGCGGGGGTTTATTTACCGGCAACACCCTTTAttt                                     >  1:1857121/2‑150 (MQ=255)
                                                                                                               gCATTACAGCCGCAAGCTAATCAGGTAGTTTTTTTTTTATGGCTGATTTTCCTTTTGCCTTTCCGGCACCCGCTCTCTCTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCa                                   <  1:340525/150‑1 (MQ=255)
                                                                                                                  ttACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGTTTCTCCGTTTGCCTTTCCGGCAGCCCCCGTCACTTTCCGGGCCCCGATTCTGGTGGTTGCTTACGGAGGCGGAGGTTTATTTACCGGCAACACCTTTTATTTCAcct                                >  1:1475045/1‑148 (MQ=255)
                                                                                                                  ttACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGTGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCAcct                                >  1:2780624/1‑148 (MQ=255)
                                                                                                                   tACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTTTTTCACCta                               >  1:3528396/1‑150 (MQ=255)
                                                                                                                   tACAGCCGCAAGCAAAACAGGCAGCTATTTCTATATGGCTGTTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCGATTCTGATGGTTGCTTACGGAGGCGGAGTTTTATTTACCGGCAACACCTTTTATTTCACCta                               >  1:2182557/1‑150 (MQ=255)
                                                                                                                      aGCCCCAAGCAAACCAGGCAGCTTTTCCATTATGGCGGATGCCCCGTTTGCCTTTCCGGCAGCCGCAGCCACTTCCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATAc                            <  2:3780508/150‑1 (MQ=255)
                                                                                                                       gCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATAcc                           <  2:1047350/150‑1 (MQ=255)
                                                                                                                       accGCAGGCAAAACAGGCAGCTGTTTCTTTATGGCTGATTCTCCTTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCGGATGCTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATAcc                           <  2:2797020/149‑1 (MQ=255)
                                                                                                                          gcaagcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACcgcg                        <  1:25888/150‑1 (MQ=255)
                                                                                                                              gcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTcc                    <  1:3144402/150‑1 (MQ=255)
                                                                                                                              gcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCCATTCTGATGGTTGCTTACGGAGGCGGGGATTTATTTACCGGCAACACCTTTTATTTCACCTTTACcgcccccc                    >  1:98238/1‑145 (MQ=255)
                                                                                                                                     aGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGACAGCCGCTGTCACTTTCGGGGTCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCCCCGgaaaaa             <  1:766486/150‑1 (MQ=255)
                                                                                                                                      ggCAGCTTTTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGCGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATATAAATACCGGCTACACCTTTTATTTCACCTATACCGCGCACCGgaaaaaa            <  2:1318686/150‑1 (MQ=255)
                                                                                                                                       gCAGCTTTTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaaaaaaa           <  1:3302071/150‑1 (MQ=255)
                                                                                                                                          gctATCTCTTTATGGCTGATCCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTCTTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtca        <  1:29168/148‑3 (MQ=255)
                                                                                                                                           ctATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtcag       <  2:556894/149‑4 (MQ=255)
                                                                                                                                           ctATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtcag       >  2:188282/2‑147 (MQ=255)
                                                                                                                                            tATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtcagc      >  2:1669143/1‑146 (MQ=255)
                                                                                                                                              tttCTTTTTGGCTGATTCCCCGTTCGCCTTTCCGGCAGGTACCGTCATTATCGGGGCCGCATGTCTGATGATTGCTTACGGAGGCGGAGATCTATTTATCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTg    <  2:748937/150‑1 (MQ=255)
                                                                                                                                               ttCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTgg   >  2:4373621/1‑150 (MQ=255)
                                                                                                                                                tcttTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGc  <  1:2329168/150‑1 (MQ=255)
                                                                                                                                                tcttTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGc  >  2:3050788/1‑150 (MQ=255)
                                                                                                                                                tcttTATGGCTGATTCCCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCGGCTGTTCTGATGTTTGCTTACGGAGGCGGAGTTTTATTTACTGGCAACTCCTTTTATTTCACCTATACAGCGCTCCGGAAAAAAATCAGCTGGc  <  1:1865867/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGC  >  CP006881/3723197‑3723490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑