Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,952 A→C K256Q (AAA→CAA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,9520AC99.8% 1613.8 / ‑5.6 412K256Q (AAA→CAA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (189/222);  minor base G (0/1);  total (189/223)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 429 total aligned reads displayed.

TGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGC  >  CP006881/3723805‑3724098
                                                                                                                                                   |                                                                                                                                                  
tgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGatcaa                                                                                                                                                  <  1:2671527/150‑1 (MQ=255)
 gCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGCAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGatcaat                                                                                                                                                 <  1:4266627/150‑1 (MQ=255)
   acaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGTCCGCGGGAATAGTTATGATGGGCTGGATGGACTACACACTCAATCCTGgtcaataa                                                                                                                                               >  2:1989594/1‑150 (MQ=255)
   acaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGatcaataa                                                                                                                                               <  1:1602402/150‑1 (MQ=255)
     atccGCCACCCCGCTTCTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaa                                                                                                                                             <  2:3966500/147‑1 (MQ=255)
     aTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaa                                                                                                                                             <  1:2847970/150‑1 (MQ=255)
        cGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaaaaa                                                                                                                                          <  2:1618262/150‑1 (MQ=255)
        cGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATaaaaaaa                                                                                                                                          <  1:2158302/150‑1 (MQ=255)
         gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAAc                                                                                                                                         >  2:2711049/1‑150 (MQ=255)
         gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAAc                                                                                                                                         <  2:2538275/150‑1 (MQ=255)
         gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAAc                                                                                                                                         >  2:4308602/1‑150 (MQ=255)
         gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGAGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAAc                                                                                                                                         <  1:498887/150‑1 (MQ=255)
          ccATCTGGCTTTTGACCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCCCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACt                                                                                                                                        <  1:3113059/150‑1 (MQ=255)
          ccATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACt                                                                                                                                        <  2:1497277/150‑1 (MQ=255)
            aTCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTc                                                                                                                                      <  1:3815849/150‑1 (MQ=255)
            aTCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTc                                                                                                                                      <  1:1652135/150‑1 (MQ=255)
            aTCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTc                                                                                                                                      <  1:1109794/150‑1 (MQ=255)
              ctcGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCa                                                                                                                                    <  1:3965687/150‑1 (MQ=255)
               tcgcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGATGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCag                                                                                                                                   >  2:2123921/4‑150 (MQ=255)
                cgcTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCaga                                                                                                                                  <  1:759563/148‑1 (MQ=255)
                   ttttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGAt                                                                                                                               >  2:1176018/1‑150 (MQ=255)
                   ttttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGAt                                                                                                                               >  2:2486547/1‑150 (MQ=255)
                    tttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATc                                                                                                                              <  2:639424/150‑1 (MQ=255)
                     ttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGATACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCa                                                                                                                             >  2:2878249/1‑150 (MQ=255)
                     ttGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCa                                                                                                                             <  2:564356/150‑1 (MQ=255)
                      tGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAGAACTTCCAGAGATCAc                                                                                                                            >  2:1137514/1‑150 (MQ=255)
                       gATCGAGCACCCTGATCCAGTGACACTGATGGGAGGAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCAGGATGTACTACACACTGAATCCAGATCAATAAAAAAACTTCCAGAGATCACt                                                                                                                           <  1:2363796/150‑1 (MQ=255)
                         ccGAGCCCCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTcc                                                                                                                         <  2:3788922/149‑1 (MQ=255)
                           gAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCt                                                                                                                       >  1:975878/1‑150 (MQ=255)
                            aGCACCCTGATACAGTGACACTGATGGGAGCAGCCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTg                                                                                                                      <  2:3850113/150‑1 (MQ=255)
                             gCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACATAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGACCAATAAAAAACGTTCCAGAGATCACTCCCTGc                                                                                                                     <  1:4411050/150‑1 (MQ=255)
                               aCCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCaa                                                                                                                   >  2:47386/1‑150 (MQ=255)
                               aCCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCaa                                                                                                                   <  1:4232852/150‑1 (MQ=255)
                                cccTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACCCTGAATCCTGCTCAATAAAAAAACTTCCAGAGGTCACCGCCTGGAAg                                                                                                                  >  2:4028874/1‑150 (MQ=255)
                                  cTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGGTCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTc                                                                                                                >  2:3000165/1‑150 (MQ=255)
                                   tGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                                                               >  2:1070578/1‑150 (MQ=255)
                                      tACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAg                                                                                                            <  1:3239658/150‑1 (MQ=255)
                                      tACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAg                                                                                                            <  1:1506476/150‑1 (MQ=255)
                                         aGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCgg                                                                                                         >  2:4148697/1‑150 (MQ=255)
                                           tGACACTGAAGGGAGCAGTCAGAAACTTAATCCCCGCTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCCATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGtg                                                                                                       >  2:2398941/1‑149 (MQ=255)
                                                    tGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgaga                                                                                              <  2:357144/150‑1 (MQ=255)
                                                      ggAGCAGCCAGAAACTTTATCCCCGTTACGCACGGCAATCTGACCGGGGGAATAGTTATGATGGGCTGGATGTACTCCCCACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCgagaga                                                                                            <  1:1683638/150‑1 (MQ=255)
                                                         gCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGGTATTCCGAGAGATCt                                                                                         >  1:1804088/1‑150 (MQ=255)
                                                         gCAGTCAGAAACCTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCGGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCt                                                                                         <  2:1906375/150‑1 (MQ=255)
                                                              cAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAACCTGATCAATAAAAAAACTACCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAg                                                                                    <  1:3706807/150‑1 (MQ=255)
                                                               aGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGa                                                                                   >  1:1763666/1‑150 (MQ=255)
                                                                  aaCTTACTCCCCGTTCCGCTGGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAACCTGATCAATAAAAAAACTTGCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGc                                                                                <  1:1954101/150‑1 (MQ=255)
                                                                   aCTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCt                                                                               >  1:929861/1‑150 (MQ=255)
                                                                     ttAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTgg                                                                             >  1:677085/1‑150 (MQ=255)
                                                                     ttAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTgg                                                                             >  2:3534430/1‑150 (MQ=255)
                                                                         tccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACAt                                                                         <  1:2508945/148‑1 (MQ=255)
                                                                         tccccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACAt                                                                         >  1:2575178/3‑150 (MQ=255)
                                                                         tccccGGTACCCCCGGCAATCTGACCGCGGGAATAGTTATGTTGGGCTGGATGTACTACCCACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACAt                                                                         <  2:1376554/148‑1 (MQ=255)
                                                                            ccGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGCCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCa                                                                      <  1:2231933/150‑1 (MQ=255)
                                                                              gTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGc                                                                    >  1:4001047/1‑150 (MQ=255)
                                                                               ttACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCg                                                                   <  2:3200162/150‑1 (MQ=255)
                                                                                 acgcTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTCCTACACACTGAATCCTGATCAATAAACAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTg                                                                 <  2:1345289/147‑1 (MQ=255)
                                                                                   gcTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGat                                                               >  2:3334080/2‑150 (MQ=255)
                                                                                   gcTCGGCAACCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACCCACTGAATCCTGATCAATAAACAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGat                                                               <  2:1769197/149‑1 (MQ=255)
                                                                                     tCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGatat                                                             <  2:1253504/150‑1 (MQ=255)
                                                                                     tCGGCAATCTGACCGCGGGAATAGTTAAGATGGGCTGGATGTACTACACACTGAATGCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGatat                                                             <  1:4564079/150‑1 (MQ=255)
                                                                                      cGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGAtata                                                            >  2:3643168/1‑150 (MQ=255)
                                                                                      cGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGAtata                                                            <  1:1225065/150‑1 (MQ=255)
                                                                                      cGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGAtata                                                            <  1:1407685/150‑1 (MQ=255)
                                                                                      cGGCAACCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGAtata                                                            <  1:3571122/150‑1 (MQ=255)
                                                                                       ggCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAg                                                           >  2:3447234/1‑150 (MQ=255)
                                                                                         cAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGACCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGcc                                                         <  2:2355652/150‑1 (MQ=255)
                                                                                          aatcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGGGAGATCTTGAAGGCGCTGGACATGCCGCGGGATATAGCCg                                                        >  2:1112475/4‑150 (MQ=255)
                                                                                          aatcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCg                                                        >  2:1518508/4‑150 (MQ=255)
                                                                                          aatcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCg                                                        <  2:815947/147‑1 (MQ=255)
                                                                                          aaCCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCg                                                        <  1:3062529/150‑1 (MQ=255)
                                                                                           cccTGTCCGCGGGAATCGTTATGATGGGCTGGATGTCCTACTCACTGAATCCTGATCAATGAATAACCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCgg                                                       <  2:3280907/149‑1 (MQ=255)
                                                                                           atcTGACCGGGGGAATAGTTATGATGGGCTGGATGTCCTACACACTGAATCCTGATCAATATAAAAACTACCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCgg                                                       <  1:536459/148‑1 (MQ=255)
                                                                                             cTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGcc                                                     <  1:1016806/150‑1 (MQ=255)
                                                                                                 ccGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCgg                                                 >  1:4629135/1‑150 (MQ=255)
                                                                                                 ccGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCgg                                                 >  1:1342808/1‑150 (MQ=255)
                                                                                                     gggAATAGTTATGGTGGGCTGGATGTACTACACACTGAATCCTGATCAGTAAAGATACTTCCAGAGATCCCTCCCTGCAGGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCTGCGTGATATAGCCGGCCTCGGATTc                                             <  1:2241791/150‑1 (MQ=255)
                                                                                                     gggAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTc                                             >  2:4440334/1‑150 (MQ=255)
                                                                                                        aaTAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACCCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCgg                                          <  2:953973/150‑1 (MQ=255)
                                                                                                          tagttatgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCa                                        <  1:392048/145‑1 (MQ=255)
                                                                                                            gttatgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTTCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa                                      <  2:871655/147‑1 (MQ=255)
                                                                                                                tgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTa                                  >  2:4583161/1‑150 (MQ=255)
                                                                                                                  atgGGTTGGCTGTACTACACACTGAATTCTGATCAATAAAACACCTTACAGAGATCACCCCCTGCAAGTCAATGGGGTTATTCCGAGTGATCTTGAAGACGCTGGTCATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAAt                                <  1:2449865/150‑1 (MQ=255)
                                                                                                                     ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAACCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGACCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGa                             <  2:1447605/150‑1 (MQ=255)
                                                                                                                     ggCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGa                             <  1:3018497/150‑1 (MQ=255)
                                                                                                                       cTGGATGTACTACACACTGTATCCTGATCAATAAAAAAACATCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGata                           <  1:593578/150‑1 (MQ=255)
                                                                                                                           aTGTACTCCACACTGAACCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCa                       <  1:959613/150‑1 (MQ=255)
                                                                                                                             gTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGACCACTCCCTGCAAGTCAAAGCGGTTATCCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCatt                     <  2:4237618/150‑3 (MQ=255)
                                                                                                                                ctACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATtttt                  <  1:1071051/149‑1 (MQ=255)
                                                                                                                                ctACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATACCGGCAAATGTAATTGATATTCATtttt                  <  1:4717394/149‑1 (MQ=255)
                                                                                                                                    acactgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGTCGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGGAAATGAAATTCAGTTTTCaaa              >  2:2258226/7‑150 (MQ=255)
                                                                                                                                      actgaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCA‑TTTTCAAAAAc           >  1:27421/5‑150 (MQ=255)
                                                                                                                                         gaaTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAActc         >  2:510375/2‑150 (MQ=255)
                                                                                                                                           aTCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAActccg       <  2:4390127/150‑3 (MQ=255)
                                                                                                                                            tCCTGATGAATAAAAAACCTTCCAGAGATCACTCACTGCAAGTCAAAGCGGTATATCCAAGAGTCCTTGATGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGc      <  1:2059698/150‑1 (MQ=255)
                                                                                                                                            tCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGc      >  1:3360635/1‑150 (MQ=255)
                                                                                                                                             cctgATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGcc     >  1:3444555/4‑150 (MQ=255)
                                                                                                                                                gATCAATAAAAACACTTCCAGAGATCACTCCCTGCAAGTCAAGGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGc  <  1:1137514/150‑1 (MQ=255)
                                                                                                                                                gATCAATAAAAAACCTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGc  <  2:1466227/150‑1 (MQ=255)
                                                                                                                                                gATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGATATTCATTTTTCAAAAACTCCGCCAGc  >  1:3661684/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGATATTCACTTTTCAAAAACTCTGCCAGC  >  CP006881/3723805‑3724098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑