Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,578,472	(A)7→6	intergenic (‑65/‑70)	U712_18365 ← / → U712_18370	Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,578,466	0	A	.	100.0%	222.5 / NA	49	intergenic (‑59/‑76)	U712_18365/U712_18370	Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (21/28);  total (21/28)

TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGT  >  CP006881/3578329‑3578588                                                                                                                                          |                                                                                                                           ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa                                                                                                                            >  5:111795/1‑138 (MQ=255) ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTATTTTCTTCCTGTCTCATTGTTTACTCACCTCAGCAACATTCTGAACACTTTTATTAAAATATATACCAACTAACCa                                                                                                                            >  6:200455/1‑138 (MQ=255)           cacCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACttttt                                                                                                                <  6:311331/139‑1 (MQ=255)                aGCGTTTACGAAGAAACTGCTGAAGGTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCCGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                           <  5:196738/139‑1 (MQ=255)                aGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                           <  3:139809/139‑1 (MQ=255)                aGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                           <  7:278605/139‑1 (MQ=255)                 gCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCaaaa                                                                                                                         >  1:249955/1‑125 (MQ=255)                 gCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCaaaa                                                                                                                         <  2:249955/125‑1 (MQ=255)                      tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATTTAGtt                                                                                                     >  2:70810/1‑139 (MQ=255)                      tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTACCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGt                                                                                                      <  1:189268/138‑1 (MQ=255)                          aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca                                                                                                 <  1:257773/139‑1 (MQ=255)                          aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca                                                                                                 <  3:76256/139‑1 (MQ=255)                           aaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTAATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca                                                                                                 <  1:237878/138‑1 (MQ=255)                              aaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGa                                                                                             <  1:101084/139‑1 (MQ=255)                                   ctgAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGATCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACATACTGaa                                                                                        <  2:7022/139‑1 (MQ=255)                                                              cttttcttCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTTAAAACTTTTCTGAAAATATATCACACCTATCC‑TAAAAACTTTTTTAGATGTAGTTTGCACAGACTTAACGAAAATATGTATTTTTTTGAgagga                                                              <  3:128191/138‑1 (MQ=255)                                                              cttttcttCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAgagga                                                              <  3:128192/138‑1 (MQ=255)                                                                    ttcCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCa                                                        >  1:51967/1‑138 (MQ=255)                                                                                  tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                          <  6:135474/138‑1 (MQ=255)                                                                                  tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                          <  1:23241/138‑1 (MQ=255)                                                                                   ttCATCACCTCAGCAACATTCTGAACCCTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACAt                                         >  6:203378/1‑138 (MQ=255)                                                                                    tcatcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGa                                       >  1:86647/1‑139 (MQ=255)                                                                                    tcatcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGa                                       >  5:257560/1‑139 (MQ=255)                                                                                      atcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                           >  4:321883/1‑69 (MQ=255)                                                                                      atcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                           <  3:321883/69‑1 (MQ=255)                                                                                      atcaCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACa                                     >  6:170184/1‑139 (MQ=255)                                                                                           cTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCg                                <  7:186631/139‑1 (MQ=255)                                                                                              aGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAgagga                                                              >  4:299587/1‑106 (MQ=255)                                                                                              aGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAgagga                                                              <  3:299587/106‑1 (MQ=255)                                                                                              aGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCt                             >  3:231611/1‑139 (MQ=255)                                                                                                  aCATTCAGAACACTTTTCATAAAATATATACAAACTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                         <  4:229646/139‑1 (MQ=255)                                                                                                     ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa                      >  5:276084/1‑139 (MQ=255)                                                                                                     ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa                      >  3:112646/1‑139 (MQ=255)                                                                                                        tGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACa                    >  3:182555/1‑138 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAAc                                           <  4:271284/113‑1 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAAc                                           >  3:271284/1‑113 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACa                    >  7:111897/1‑136 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                  <  5:200455/138‑1 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                  <  6:117684/138‑1 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                  <  6:111795/138‑1 (MQ=255)                                                                                                               ttttCTGATAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                          <  5:302755/109‑1 (MQ=255)                                                                                                               ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                          >  6:302755/1‑109 (MQ=255)                                                                                                               ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTg                        <  6:228445/127‑1 (MQ=255)                                                                                                               ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTg                        >  5:228445/1‑127 (MQ=255)                                                                                                               ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                  >  7:224807/1‑133 (MQ=255)                                                                                                               ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                  <  8:224807/133‑1 (MQ=255)                                                                                                               ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACt            >  6:293293/1‑139 (MQ=255)                                                                                                                      aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAgg      <  4:253741/138‑1 (MQ=255)                                                                                                                         atatatACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCtgt  >  2:257860/1‑139 (MQ=255)                                                                                                                            tataCAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAgg      <  7:285500/132‑1 (MQ=255)                                                                                                                            tataCAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAgg      >  8:285500/1‑132 (MQ=255)                                                                                                                                   ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                         >  6:199426/1‑106 (MQ=255)                                                                                                                                   ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                         <  5:199426/106‑1 (MQ=255)                                                                                                                                          |                                                                                                                           TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGT  >  CP006881/3578329‑3578588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑