| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,209 | +T | intergenic (‑41/‑488) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,205 | 1 | . | T | 84.2% | 95.0 / 14.8 | 42 | intergenic (‑37/‑492) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base . (1/5); new base T (17/15); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑C‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAA > CP006881/3725068‑3725321 | tGAGCCGAATAGACCGCCGCAGCCGCAGTTATTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACAACCCTCCGCGGCATTAAGGCAGG‑CAGc‑ca > 3:99306/1‑138 (MQ=255) gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑C‑tt > 7:321536/1‑139 (MQ=255) gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑C‑tt > 7:75082/1‑139 (MQ=255) gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGG‑CAGC‑C‑tt > 5:310882/1‑139 (MQ=255) aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑C‑tttttag > 3:150519/1‑135 (MQ=255) ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCGGCCCGtttt > 1:159320/1‑139 (MQ=255) ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGttt > 5:268720/1‑138 (MQ=255) ttCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGcc < 1:34706/138‑1 (MQ=255) ttCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGcc < 8:321536/138‑1 (MQ=255) ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGCAAAGACGGCCGAtt > 4:121211/1‑139 (MQ=255) ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 6:59322/1‑139 (MQ=255) ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 2:59642/1‑139 (MQ=255) ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CGTTTTT‑GCAGCCCGTTTTCTAAGCCGCAGCCCGCAAGAAAATACGGCCGAtt > 8:139905/1‑139 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCCAGAAAAGACGGCCGAgttagtt > 6:26501/1‑135 (MQ=255) aaaccccacccacACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTGCTTTTTGGCAGG‑CAGC‑CTTTTTC‑GCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAACGACGGCCGAtttcttttct < 1:41696/134‑1 (MQ=255) aaaGACCAACCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CCGC‑CTTTTTA‑GCAGCCCTTTTTCTCAGCCGCAGCCCGCAACAAACGACGGCCGAtttcttttct < 3:34100/139‑1 (MQ=255) aGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg > 3:85162/1‑139 (MQ=255) aGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg > 4:300285/1‑139 (MQ=255) aTCCACCCCCGTAAAGATACAAGCAACCGGTTC‑ATAGAACAACATCCCTCCGCTGCTGTTTGGCGGG‑CAGC‑CGTTTTC‑GCAGCCCGTTTTGTCAGCCGCGGCCCGCAAGAGAAGGCGGCCGATGTCTTTGCTCGctatc > 4:43261/1‑135 (MQ=255) tAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑GAGC‑CTTTTTA‑GCAGCCCGTTTTATCCGCCGCAGCCCGCAAGAAAAGCCGGCCGATTTCTTTTGTCGCAAACTAGCGGctcaa > 5:352098/1‑137 (MQ=255) acaTACACGCAAACGTTTC‑ATACAAAAACACCCCTCCGCTTGTTTTTGGCAGG‑CAGC‑CTTTTTA‑CCACCCCGTTTTCACCCCCCCCCCCCGCAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 5:59322/137‑1 (MQ=255) aCAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCCAACTAGCGGCTCTAGCAAAAc > 4:171456/1‑139 (MQ=255) aCAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTGTAGCaaaa > 6:336577/1‑138 (MQ=255) aCAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa > 7:347413/1‑138 (MQ=255) aCAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 3:273990/1‑139 (MQ=255) aCAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CGTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGTTTTCTCGCAAAATAGCGGCTCGAGCaaaa > 5:214196/1‑138 (MQ=255) aCAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTGCGTTTTGGCAGG‑CAGC‑CGGTTTA‑GCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGACGGCCGATTTGTTGTGTCGCCAACTAGCGGCTCGAGCaaacc > 6:277896/1‑137 (MQ=255) aaGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAATAGCGGCTCTAGCAAAAcg > 3:332048/1‑137 (MQ=255) gaaaaCGTTTC‑AAACAAAAACATCCCCCCGCTTTTTTTTGGCAGG‑CAGC‑CTTTTTC‑GCCGCCCGTTTTCTCCGCCGCCGCCCCCAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 8:347413/137‑1 (MQ=255) gCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CCGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:11250/139‑1 (MQ=255) gCAAACGTTTA‑ATATAAAAACATCCCTCCGCTTCTTTTTGGCCGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCACCCGCAGCCCCCAATAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:197592/139‑1 (MQ=255) ccAAAGGTTTC‑ATACAAAAACATCCCCCCGCTTCTTTTTGGCCGG‑CCGC‑CTTTTTC‑GCCGCCGGTTTTCTCAGCCGCAGCCCCAAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 3:303777/138‑1 (MQ=255) ccAAACTTTTA‑ATACAAAAACACCCCTCCGATTTTTTTTGGCCGG‑CACCAT‑TTTTA‑GCCCCCCTTTTACCCCCCCGCCCCCCCAAAAAAAACACGGCCGATTTCTTTTCTCGCAAAAAAGCGGCTCTAGCAAAACTCAt < 5:90459/138‑1 (MQ=255) aaagttttC‑ATAAAAAAACACCCCCCCGCTTATTTTGGCCCGG‑CCGCTT‑TTTTC‑CCCCCCCGTTTGCTCCCCCCCCGCCCGCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 5:336577/134‑1 (MQ=255) aaaCGTTTC‑AAACAAAAACAACCCTCCGCTTCTTTTTGGCCGG‑CACC‑CTTTTTA‑GCAGCCCGTTTTCTCCGCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa < 7:7458/139‑1 (MQ=255) aaCGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa > 4:349867/1‑138 (MQ=255) ccGTTTC‑ATCCAAAAACACCCCACGGCTTTTTTTTGCCCGC‑CCGC‑CTTTTTC‑CCCGCCCGTTTTCCCCGCCGCCCCCCGACAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 8:75082/138‑1 (MQ=255) ccGTTTC‑ATACAAAAACACCCCCCCGCTTCTTTTGGGCCGC‑CCGC‑C‑TTTTACCCCGCCCGTTTTCCCAGCCCCCCCCCCAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 4:150519/138‑1 (MQ=255) ccGTTAC‑ATACAAAAAAAGCCCTCCGCTTATTTGTGCCCGC‑CCGC‑CTTTTTC‑GCCGCCCGTTTTCCCCGCCGCACCCCGCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 8:350348/138‑1 (MQ=255) aCGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAc > 3:164273/1‑138 (MQ=255) aCGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCCCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 7:220345/139‑1 (MQ=255) aCGTTAC‑AAAAAAAAACAACCCCCCCCTTATTTTTGGCCGC‑CCCC‑C‑TTTTACCCCCCCCGTTTCCCCCCCCCCCCCCCCCACCAACACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 4:273990/139‑1 (MQ=255) agTTTCAATACAAAAAAATCCCTCCTCTTTTTTTTGGCTGG‑CCGCTT‑TTTTC‑GCACCCGTTTTTCTCCGCCCCCGCCCCAAAAAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 4:164273/138‑1 (MQ=255) tAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCGGC‑T‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTCTCGCAAACTAGCGGCTCGAGCAAAACTCATGACTCTATGa > 1:278554/1‑137 (MQ=255) cATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTgg < 6:120603/139‑1 (MQ=255) cATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTgg < 3:349867/139‑1 (MQ=255) cGCTTCTTTTTGGCAGG‑CAGC‑CTTTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTGTGGCCAAATAGCGGCTCGAGCAACACTCATGCCTAGATTGTATACCGGGGaaatataa > 4:27638/1‑134 (MQ=255) | TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTC‑ATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAGC‑C‑TTTTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAA > CP006881/3725068‑3725321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |