Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,121	T→A	intergenic (‑235/‑66)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,121	0	T	A	100.0%	256.7 / NA	78	intergenic (‑235/‑66)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (34/44);  total (34/44)

CTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTT‑TTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAAC  >  CP006881/3722990‑3723231                                                                                                                                      |                                                                                                               cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                                                           <  5:311533/138‑1 (MQ=255) cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                                                                                           >  6:153615/1‑138 (MQ=255)    tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                                                        <  8:107592/138‑1 (MQ=255)    tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                                                                                                        <  6:331572/138‑1 (MQ=255)      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATACATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                                      <  4:302169/138‑3 (MQ=255)      ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                                      <  1:93635/138‑3 (MQ=255)          tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                                 <  7:40396/139‑1 (MQ=255)           cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTCTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                                 >  5:262224/1‑138 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  5:156284/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  2:284771/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  2:145011/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  5:218801/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  5:153615/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  7:3174/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  8:349649/139‑1 (MQ=255)               aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  3:192760/139‑1 (MQ=255)               aTCCCGCTTAATCAGATTAAGAATACGCTTTCATCATAAATCCTGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  6:143918/139‑1 (MQ=255)               aTCCCGCATATTCAGATTAAGAATACGCTTTCCTCATAAATCATGATAGCGGTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATCTAACATGTAGACAAAAAATCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  8:64989/139‑1 (MQ=255)                  ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTCTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                          <  6:262224/138‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                       <  2:331774/139‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                       <  4:8445/139‑1 (MQ=255)                    gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                       >  1:259860/1‑139 (MQ=255)                     cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc                                                                                      >  6:314124/1‑139 (MQ=255)                        aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                                                     <  6:103189/137‑1 (MQ=255)                        aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt                                                                                     <  5:139553/137‑1 (MQ=255)                         ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata                                                                                  <  1:119083/139‑1 (MQ=255)                          tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATTTAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  2:327447/1‑128 (MQ=255)                          tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATTTAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  1:327447/128‑1 (MQ=255)                          tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  3:285118/128‑1 (MQ=255)                          tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  4:285118/1‑128 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  1:145258/1‑124 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  1:80360/1‑124 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  2:145258/124‑1 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  3:208643/1‑124 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  4:208643/124‑1 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  2:80360/124‑1 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                             >  2:64805/1‑139 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCATCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  6:80417/124‑1 (MQ=255)                              aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCATCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTAACAata                                                                                            >  5:80417/1‑124 (MQ=255)                                 aagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCt                                                                           >  6:234833/2‑138 (MQ=255)                                   gaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                                      <  2:84248/109‑3 (MQ=255)                                   gaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                                                                                                      >  1:84248/1‑107 (MQ=255)                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                                                    >  1:172388/1‑138 (MQ=255)                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                                                    >  1:324275/1‑138 (MQ=255)                                        cGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                                                    >  5:180191/1‑138 (MQ=255)                                              catcatAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                                                              >  6:68709/1‑138 (MQ=255)                                              catcatAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaaga                                                              >  6:74046/1‑138 (MQ=255)                                                tcatAAATCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                           <  4:124821/139‑1 (MQ=255)                                                     aaTCATGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAgg                                                       >  3:257423/1‑138 (MQ=255)                                                      atcaTGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaa                                                     <  2:324275/136‑1 (MQ=255)                                                      atcaTGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaa                                                     <  6:90516/136‑1 (MQ=255)                                                         aTGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                           >  2:55569/1‑130 (MQ=255)                                                         aTGATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaaag                                                           <  1:55569/130‑1 (MQ=255)                                                         aTGATACCGTTTTCGTCAACT‑ATTTTATTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGAGCTAAACTATGCTCAATCATGAAAGAAAGCAGGaa                                                     >  8:11269/1‑136 (MQ=255)                                                           gATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  3:324395/95‑1 (MQ=255)                                                           gATAGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  4:324395/1‑95 (MQ=255)                                                              aGCGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTAAACTATACTCAATCATGAAAGAAAGCAGGAAAAGACAAt                                             >  3:106135/1‑139 (MQ=255)                                                                cGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  1:310384/1‑90 (MQ=255)                                                                cGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  1:305910/1‑90 (MQ=255)                                                                cGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            >  1:8217/1‑90 (MQ=255)                                                                cGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  2:310384/90‑1 (MQ=255)                                                                cGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  2:8217/90‑1 (MQ=255)                                                                cGTTTTCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                            <  2:305910/90‑1 (MQ=255)                                                                  ttttCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAACTCAGGAAAAGACACTGGa                                          >  6:74324/1‑138 (MQ=255)                                                                  ttttCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa                                          >  8:263894/1‑138 (MQ=255)                                                                    ttCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                                                                <  6:137646/98‑1 (MQ=255)                                                                    ttCGTCAACTATTTTT‑TTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                                                                >  5:137646/1‑98 (MQ=255)                                                                           actattttt‑ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                                <  5:314124/134‑1 (MQ=255)                                                                               ttttt‑ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtta                             >  4:240488/2‑137 (MQ=255)                                                                                 ttt‑ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                           <  5:234833/138‑1 (MQ=255)                                                                                       aGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaaagg                      <  1:64805/138‑3 (MQ=255)                                                                                            aaTAGTTTGATATAACATGTAGACAAAAATTAGTAAAAATTAATTGTGAAATACTTCACAATATAGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAAc                <  3:4392/139‑1 (MQ=255)                                                                                                 tttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTat           >  2:16680/1‑138 (MQ=255)                                                                                                   tGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                                                 >  3:72890/1‑97 (MQ=255)                                                                                                   tGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaaga                                                 <  4:72890/99‑3 (MQ=255)                                                                                                           catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAAc                >  7:56292/5‑124 (MQ=255)                                                                                                           catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAAc                <  8:56292/120‑1 (MQ=255)                                                                                                           catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaa  >  8:349989/5‑137 (MQ=255)                                                                                                                                      |                                                                                                               CTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTT‑TTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAAC  >  CP006881/3722990‑3723231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑