Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,183	A→G	intergenic (‑297/‑4)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,183	0	A	G	100.0%	332.1 / NA	88	intergenic (‑297/‑4)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/39);  total (49/39)

AGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTA  >  CP006881/3723051‑3723313                                                                                                                                      |                                                                                                                                   aGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTAAACTATACTCAATCATGAAAGAAAGCAGGAAAAGACAAt                                                                                                                               >  3:106135/1‑139 (MQ=255)     ttttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAACTCAGGAAAAGACACTGGa                                                                                                                            >  6:74324/1‑138 (MQ=255)     ttttCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa                                                                                                                            >  8:263894/1‑138 (MQ=255)              actatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCa                                                                                                                  <  5:314124/134‑1 (MQ=255)                  tttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtta                                                                                                               >  4:240488/2‑137 (MQ=255)                    tttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                                                                                                             <  5:234833/138‑1 (MQ=255)                         aGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaaagg                                                                                                        <  1:64805/138‑3 (MQ=255)                              aaTAGTTTGATATAACATGTAGACAAAAATTAGTAAAAATTAATTGTGAAATACTTCACAATATAGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAAc                                                                                                  <  3:4392/139‑1 (MQ=255)                                   tttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTat                                                                                             >  2:16680/1‑138 (MQ=255)                                             catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAAc                                                                                                  >  7:56292/5‑124 (MQ=255)                                             catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAAc                                                                                                  <  8:56292/120‑1 (MQ=255)                                             catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaa                                                                                    >  8:349989/5‑137 (MQ=255)                                                                        attGTGAAATACTTAACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGGAGGAAAAGACAAAGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaa                                                         <  7:11269/137‑1 (MQ=255)                                                                            tgAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCg                                                    <  8:52860/139‑1 (MQ=255)                                                                                      cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAcaa                                                                              <  6:14994/103‑3 (MQ=255)                                                                                      cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAcaa                                                                              >  5:14994/1‑101 (MQ=255)                                                                                      cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCtt                                          >  7:89185/1‑138 (MQ=255)                                                                                      cacaATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCt                                           <  4:257423/138‑1 (MQ=255)                                                                                              cGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTAAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAt                                  >  1:136663/1‑139 (MQ=255)                                                                                                tGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                                      >  1:250989/1‑128 (MQ=255)                                                                                                tGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                                      <  2:250989/133‑6 (MQ=255)                                                                                                tGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAttt                                >  1:25775/1‑139 (MQ=255)                                                                                                tGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAttt                                >  4:202446/1‑139 (MQ=255)                                                                                                tGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCAttt                                >  4:335823/1‑139 (MQ=255)                                                                                                     taCTATGCTCAATCATGAAATAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATTCTTTGAAAAAACAAAAAATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  3:271230/138‑1 (MQ=255)                                                                                                     taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  5:68709/138‑1 (MQ=255)                                                                                                     taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  3:240488/138‑1 (MQ=255)                                                                                                     taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  5:74046/138‑1 (MQ=255)                                                                                                     taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  2:172388/138‑1 (MQ=255)                                                                                                     taCTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  7:349989/138‑1 (MQ=255)                                                                                                     taCTATGCTCAATCATGAAAGAAACCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  5:75377/138‑1 (MQ=255)                                                                                                       cTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCa                          <  7:263894/138‑1 (MQ=255)                                                                                                       cTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAg                         <  3:202446/139‑1 (MQ=255)                                                                                                         aTGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGtt                                                                                                      <  8:63687/60‑1 (MQ=255)                                                                                                         aTGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGtt                                                                                                      >  7:63687/1‑60 (MQ=255)                                                                                                         aTGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta                       >  7:187787/1‑137 (MQ=255)                                                                                                          tgatCAATCATGAAAGAAAGAAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTTAAAAAACAAAACATATTCGCTACAAGCAAAATCCGTTATGTGCTTCTGTCCATTTTGGCCAgta                       >  1:81175/4‑136 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGATAAGACAATGGAAACTCAAGCATTACAAAACGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAg                         <  7:99222/136‑1 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaaagg                                                                                                        <  5:26864/57‑3 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGTTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            >  7:244442/1‑133 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGTTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  8:244442/133‑1 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGtt                                                                                                      >  5:316868/1‑59 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGtt                                                                                                      <  6:316868/59‑1 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGtt                                                                                                      >  7:286075/1‑59 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGtt                                                                                                      <  8:286075/59‑1 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                                      <  4:18706/123‑6 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                                      <  5:325380/123‑6 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                                      >  3:18706/1‑118 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCttcggt                                      >  6:325380/1‑118 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            >  4:132264/1‑133 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  2:267364/133‑1 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  5:70150/133‑1 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  3:132264/133‑1 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            >  1:267364/1‑133 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            >  6:70150/1‑133 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta                       >  6:83781/1‑136 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta                       >  6:208240/1‑136 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta                       >  6:155363/1‑136 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta                       >  2:64087/1‑136 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgta                       <  5:83781/138‑3 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTAt                      >  2:337545/1‑139 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGATTCGGTCCATTTTGGCCAgta                       >  8:308317/1‑136 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGATTCGGACCATTTTGGCCAg                         >  3:240428/1‑136 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAAACCGTTATGTGCATCGGTCCATTTTGGCCAgta                       >  6:108929/1‑136 (MQ=255)                                                                                                          tGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTAAGCTTTGAAAAAACAAAACATATTCGCTTCTAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAgt                        <  5:108929/137‑2 (MQ=255)                                                                                                               aatcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAAt                                                       <  1:261661/98‑1 (MQ=255)                                                                                                               aatcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAAt                                                       >  2:261661/4‑101 (MQ=255)                                                                                                               aatcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTa                 >  2:187488/4‑139 (MQ=255)                                                                                                               aatcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTa                 >  3:73238/4‑139 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            >  7:37659/2‑126 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  8:37659/125‑1 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTAtt               >  6:151877/2‑138 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTAtt               >  3:217079/2‑138 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTAtt               >  4:52389/2‑138 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTAtt               >  1:128441/2‑138 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTAtt               >  1:186696/2‑138 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAATAGCTTCAAGCAAAATACGTAATGTGCTTCGGTCCATTTTGGCCAGTATATTTAtt               >  8:99222/2‑138 (MQ=255)                                                                                                                 tcATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAACCATTACAAAAGGTTGAACAGTATGCTTAGAAAAAACAAAACATATACGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTAtt               >  3:343355/2‑138 (MQ=255)                                                                                                                    tgaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaa                                                         <  1:246511/92‑1 (MQ=255)                                                                                                                    tgaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaaa                                                         >  2:246511/3‑94 (MQ=255)                                                                                                                    tgaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCATAATCCGTTATGTGCTTCGGTCCATTTTGGc                            >  4:327653/3‑123 (MQ=255)                                                                                                                    tgaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGc                            <  3:327653/121‑1 (MQ=255)                                                                                                                    tgaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTggt            >  8:171525/3‑138 (MQ=255)                                                                                                                    tgaaagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGAAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTggt            >  8:205372/3‑138 (MQ=255)                                                                                                                       aagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAATGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCt                                           <  4:257081/105‑1 (MQ=255)                                                                                                                       aagaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCt                                           >  3:257081/1‑105 (MQ=255)                                                                                                                         gaaagCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTgg       <  6:180191/139‑1 (MQ=255)                                                                                                                              cAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTa  <  1:187488/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   AGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTA  >  CP006881/3723051‑3723313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑