Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	1,071,581	C→T	P272L (CCG→CTG)	U712_05275 →	Putative ABC transporter substrate‑binding lipoprotein yhfQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,071,581	0	C	T	100.0%	172.4 / NA	53	P272L (CCG→CTG)	U712_05275	Putative ABC transporter substrate‑binding lipoprotein yhfQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/28);  total (25/28)

GCAATAGCGATTCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCCGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGCGCCGCAGTGTAGACGGGGCGAATGCCATTTTGGACGAGCT  >  CP006881/1071446‑1071707                                                                                                                                        |                                                                                                                               gCAATAGCGATTCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGAt                                                                                                                             >  1:225465/1‑139 (MQ=255)   aaTAGCGATTCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATc                                                                                                                            >  6:142941/1‑138 (MQ=255)   aaTAGCGATTCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATc                                                                                                                            >  3:12281/1‑138 (MQ=255)    aTAGCGTTACACGCATGGGCGGCGAGTCTTTTAATATTAAAATGACACGGGACCCGCTGCTTAAAACAGATCGGGATGTGATCATCCTGATGACAGGAAAAACACATGACCTCGACGCCGACGGTAAACGCCTGATCGa                                                                                                                          <  2:212440/139‑1 (MQ=255)          aTTCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGaa                                                                                                                    >  1:103057/1‑139 (MQ=255)          aTTCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGaa                                                                                                                    >  5:182889/1‑139 (MQ=255)          aTTCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGaa                                                                                                                    >  1:212440/1‑139 (MQ=255)           ttCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGCCGGTAAACGCCTGATCGAAAAGAAt                                                                                                                   >  6:12103/1‑139 (MQ=255)                     ggcggcTACTCTGTTAATATCAAAATGACACGGGAGCAGCTGCTGAAAACATATCCGGAGGTTATCATCCTGATGACAGTAAAAACAGAGGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGa                                                                                                         <  4:252471/139‑1 (MQ=255)                     ggcggcGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGa                                                                                                         <  2:225465/139‑1 (MQ=255)                       cggcgACTCTGTTAATATCAAAATGACACTGCCGCAGCTGCTGAAAACAGATCCGGAGGTGATCAACCGGATGACAGGAAAAACAGATGACCTCCACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGaaa                                                                                                       <  7:140904/139‑1 (MQ=255)                            aCTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACt                                                                                                  >  4:68005/1‑139 (MQ=255)                                  tgAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGCCCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAgg                                                                                             >  7:350644/1‑138 (MQ=255)                                       atGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTCGAAAAAACTGAAGGCAgag                                                                                        >  5:190368/1‑136 (MQ=255)                                        tGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGa                                                                                       >  2:297822/1‑138 (MQ=255)                                          aaaaTGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGaaa                                                                                     >  1:340747/1‑138 (MQ=255)                                           aaaTGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTTATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGaaaaa                                                                                   <  3:254312/139‑1 (MQ=255)                                             ttcacacTGGCGCAGCGGCTTAAAACAGATCCGTTTGTGTTCATCCTGATCACAGTAAAAACCGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACg                                                                                 <  5:12103/136‑1 (MQ=255)                                             atcacacTGGACCCGCGCCTAAAAACAGTCCGGGATGTCATCATCCTTATCACACAAAAAAACTATCACCGCCCCGCCGCCGGTAAACGCCTGATCGAAAAGAATGTCCTTTGCAAAAAACTGAAGGCAGTGAAAAACg                                                                                 <  2:315877/136‑1 (MQ=255)                                             aTGACACTGTCGCAGCTGCTTAAAACAGATCCGGATGTTATCATCCTGATGAAAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACg                                                                                 <  8:173972/139‑1 (MQ=255)                                                acacTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACggg                                                                               <  5:142941/138‑1 (MQ=255)                                                    tGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATg                                                                           >  8:13712/1‑138 (MQ=255)                                                      gAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGtat                                                                        <  7:195658/139‑1 (MQ=255)                                                      gAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGtat                                                                        <  5:171106/139‑1 (MQ=255)                                                              gctgAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTg                                                                >  7:159358/1‑139 (MQ=255)                                                               ctgAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTgg                                                               <  1:297822/139‑1 (MQ=255)                                                                tgAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACg                                                                                 >  4:117985/1‑120 (MQ=255)                                                                tgAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACg                                                                                 <  3:117985/120‑1 (MQ=255)                                                                    aaCCGATCCGTCTGTGAGCATCCTGATTCCCGGAAAACCCGAGCACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCgg                                                          <  7:206867/139‑2 (MQ=255)                                                                    aaCAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGt                                                          <  8:200415/139‑1 (MQ=255)                                                                         aTCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAgg                                                                                             <  8:183429/99‑1 (MQ=255)                                                                         aTCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAgg                                                                                             <  6:123099/99‑1 (MQ=255)                                                                         aTCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAgg                                                                                             >  7:183429/1‑99 (MQ=255)                                                                         aTCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAgg                                                                                             >  5:123099/1‑99 (MQ=255)                                                                         aTCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCAt                                                                            <  7:39785/116‑1 (MQ=255)                                                                         aTCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCAt                                                                            >  8:39785/1‑116 (MQ=255)                                                                         aTCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGgt                                                     <  3:68005/139‑1 (MQ=255)                                                                                    atcatcCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTgc                                          >  7:69876/1‑139 (MQ=255)                                                                                        tcgttttgaCATTAAAAACTGATGACACGGACGCGGTAGGTAAACGCCTGATCGTAAAGAATGTGCTTTGTAAAAATCTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccg                                      <  6:190368/133‑1 (MQ=255)                                                                                        tcCTGATTACATTAAAAAAAGAGCACCTCGACGCCGACGGTAAACGCCTTATCGAAAAGAATGTCCTTTGGAAAAACCTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccg                                      <  5:71985/139‑1 (MQ=255)                                                                                        tcCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccg                                      <  2:103057/139‑1 (MQ=255)                                                                                        tcCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccg                                      <  7:117625/139‑1 (MQ=255)                                                                                        tcCTGATGACAGCAAAAACCGATCACCCCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccg                                      <  6:182889/139‑1 (MQ=255)                                                                                         ccTGATGACAGGAAAAACAGATGACCTCGACGCCGATGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccgc                                     >  5:204324/1‑139 (MQ=255)                                                                                            tatgcCAGTAAAAACAGAGCACCTCTACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccg                                      <  4:271991/134‑1 (MQ=255)                                                                                            gatgaCAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGCGCCGCAgt                                  >  5:95827/1‑139 (MQ=255)                                                                                                    gAAAAACAGATGACCTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGCGCCGCAGTGTAGACgc                          >  3:271991/1‑138 (MQ=255)                                                                                                                  cTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGTAAAAAACTGAAgg                                                                                             <  2:6078/58‑1 (MQ=255)                                                                                                                  cTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAgg                                                                                             >  1:6078/1‑58 (MQ=255)                                                                                                                  cTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccg                                      <  4:301686/113‑1 (MQ=255)                                                                                                                  cTCGACGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGcgccg                                      >  3:301686/1‑113 (MQ=255)                                                                                                                       cGCCGACGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGCGCCGCAGTGTAGACGGGGCGAATGCCATTTTGGAc       <  1:201938/139‑1 (MQ=255)                                                                                                                            aCGGTAAACGCCTGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGCGCCGCAGTGTAGCCGGGGCGAATGCCATTTTGGACGAGCt  >  6:195232/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                               GCAATAGCGATTCAAGCAATGGCGGCGACTCTGTGAATATGAAAATGACACTGGAGCAGCTGCTGAAAACAGATCCGGATGTGATCATCCTGATGACAGGAAAAACAGATGACCTCGACGCCGACGGTAAACGCCCGATCGAAAAGAATGTCCTTTGGAAAAAACTGAAGGCAGTGAAAAACGGGCATGTATACCACGTGGATCGTGCGGTGTGGTCTCTGCGCCGCAGTGTAGACGGGGCGAATGCCATTTTGGACGAGCT  >  CP006881/1071446‑1071707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑