Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,783	C→T	F199F (TTC→TTT)	U712_19195 →	putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,783	0	C	T	100.0%	199.1 / NA	60	F199F (TTC→TTT)	U712_19195	putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/39);  total (21/39)

GAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCAT  >  CP006881/3723649‑3723917                                                                                                                                       |                                                                                                                                       gAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAac                                                                                                                                    <  1:35765/139‑1 (MQ=255) gAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGGGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAac                                                                                                                                    <  7:78963/139‑1 (MQ=255)  aattGTTTTTCAGAGGTATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTGATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAaca                                                                                                                                   <  5:225416/136‑1 (MQ=255)  aattGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAaca                                                                                                                                   <  6:64648/136‑1 (MQ=255)       tttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCa                                                                                                                              <  3:356804/139‑1 (MQ=255)       tttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCa                                                                                                                              <  1:301830/139‑1 (MQ=255)       tttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCa                                                                                                                              <  5:350319/139‑1 (MQ=255)       tttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCa                                                                                                                              <  8:262783/139‑1 (MQ=255)       tttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCa                                                                                                                              <  1:175637/139‑1 (MQ=255)        ttttCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCa                                                                                                                              <  6:9288/138‑1 (MQ=255)             agagGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGcc                                                                                                                         <  7:110334/138‑1 (MQ=255)             agagGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGcc                                                                                                                         <  3:103740/138‑1 (MQ=255)               agGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAat                                                                                                                      <  3:147234/139‑1 (MQ=255)               agGAATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCAATACAATTTATATAAAAGGGGAAGGAACAAAGCTTTTTAACATTATGCTTTTCGTTTTCTTCTTCTTTATTTCCCGCCTTGAAAACAGCAATTCCaa                                                                                                                       >  2:175637/1‑138 (MQ=255)                 gaaTGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAatat                                                                                                                    >  4:244689/3‑139 (MQ=255)                    tGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATAtgtg                                                                                                                 >  4:158634/1‑139 (MQ=255)                      cTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGca                                                                                                               <  4:99683/139‑1 (MQ=255)                         tgCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACAt                                                                                                            >  8:310824/1‑139 (MQ=255)                         tgCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACAt                                                                                                            >  4:19840/1‑139 (MQ=255)                         tgCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGCAAGGAGCAAAGCTTTTTACGATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACAt                                                                                                            >  1:344748/1‑139 (MQ=255)                                ggCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCg                                                                                                         <  2:328103/135‑1 (MQ=255)                                ggCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCg                                                                                                         >  1:328103/1‑135 (MQ=255)                                ggCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCa                                                                                                      >  1:242642/1‑138 (MQ=255)                                 gCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCAtc                                                                                                    <  2:242642/139‑1 (MQ=255)                                   ttGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCAtctc                                                                                                  >  8:109302/1‑139 (MQ=255)                                   ttGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCAtctc                                                                                                  >  1:139666/1‑139 (MQ=255)                                      tgtgCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCt                                                                                               >  5:12848/1‑139 (MQ=255)                                        tgCCTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCttt                                                                                             >  1:200151/1‑139 (MQ=255)                                           cTCGCCTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGa                                                                                          >  8:58363/1‑139 (MQ=255)                                                cTTTTTCATTCCAATGTCTCTCCAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTCCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                     <  1:314142/139‑1 (MQ=255)                                                cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                     <  7:109302/139‑1 (MQ=255)                                                cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                     <  5:150597/139‑1 (MQ=255)                                                cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                     <  5:161028/139‑1 (MQ=255)                                                cTTTTTCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAg                                                                                     <  6:12848/139‑1 (MQ=255)                                                   tttCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTAGCATGATGCTTTTCGTTTTCTGCTTCTTTATATCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCa                                                                                   <  2:344748/138‑1 (MQ=255)                                                   tttCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCa                                                                                   <  8:306997/138‑1 (MQ=255)                                                   tttCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCa                                                                                   <  4:102007/138‑1 (MQ=255)                                                   tttCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCa                                                                                   <  7:344342/138‑1 (MQ=255)                                                     tCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCAcc                                                                                 <  7:310824/138‑1 (MQ=255)                                                     tCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCAcc                                                                                 <  7:58363/138‑1 (MQ=255)                                                     tCATTCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCAcc                                                                                 <  3:19840/138‑1 (MQ=255)                                                         tCCAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGa                                                                             >  7:156652/1‑138 (MQ=255)                                                           cAATGTCTCTCAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATa                                                                           <  8:156652/138‑1 (MQ=255)                                                                  tctcAAAGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACa                                                                   >  6:100185/1‑139 (MQ=255)                                                                    tcaaaGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACa                                                                   <  5:100185/135‑1 (MQ=255)                                                                    tcaaaGGGGAAGGAGCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACa                                                                   <  3:244689/135‑1 (MQ=255)                                                                            gaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAg                                                         <  5:261940/139‑1 (MQ=255)                                                                            gaaggagCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAg                                                         <  2:139666/139‑1 (MQ=255)                                                                              aggagCAAATCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCa                                                       <  3:190379/139‑1 (MQ=255)                                                                                  gCAAAGCTTTTTACCATGATGCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCa                                                   <  3:19039/139‑1 (MQ=255)                                                                                                 atgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCg                                    <  1:119861/139‑1 (MQ=255)                                                                                                  tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGa                                                                             >  2:203169/1‑97 (MQ=255)                                                                                                  tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGa                                                                             <  1:203169/97‑1 (MQ=255)                                                                                                  tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                   >  4:126227/1‑139 (MQ=255)                                                                                                  tgatgCTTTTCGTTTTCTGCTTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCGTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                   >  5:223561/1‑139 (MQ=255)                                                                                                  tgatgCTTTTCGTTTTCTGCGTCTTTATTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGCTCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGt                                   >  6:235697/1‑139 (MQ=255)                                                                                                                           taTTTCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCggg           <  6:157678/137‑1 (MQ=255)                                                                                                                             tttCCGGCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGaa         <  8:77029/138‑1 (MQ=255)                                                                                                                                  ggCTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGtta   >  6:104082/1‑137 (MQ=255)                                                                                                                                    cTTTGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTat  >  6:199792/1‑138 (MQ=255)                                                                                                                                       |                                                                                                                                       GAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGCCTTTTTCATTCCAATGTCTCTTAAAGGGGAAGGGGCAAAGCTCTTTACCATGATGCTTTTCGTTTTCTGCTTCTTCATTTCCGGCTTCGAACACAGCATTGCCAATATGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCAT  >  CP006881/3723649‑3723917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑