Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,939 G→A T251T (ACG→ACA)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,9390GA97.5% 126.9 / NA 40T251T (ACG→ACA) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/11);  minor base C (1/0);  total (29/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGA  >  CP006881/3723803‑3724071
                                                                                                                                        |                                                                                                                                    
tgtgCACATTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt                                                                                                                                    <  5:104082/139‑1 (MQ=255)
tgtgCACATTCGCCATCTCGCTATTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACt                                                                                                                                    <  8:352440/139‑1 (MQ=255)
    cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACCCTGAAt                                                                                                                                >  2:330610/1‑139 (MQ=255)
    cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                                >  1:295302/1‑139 (MQ=255)
    cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                                >  3:120803/1‑139 (MQ=255)
    cacaTTCGCCATCTCGCTTTTGATCGAGCACCCTGATACAGTAACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAAt                                                                                                                                >  2:63976/1‑139 (MQ=255)
           gCCATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGatc                                                                                                                         <  2:295302/139‑2 (MQ=255)
            ccATCTCGCTTTTGATCGAGCACCCTGATACAGTGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTCCTACACACTGAATCCTGatc                                                                                                                         >  2:162496/1‑137 (MQ=255)
                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTcc                                                                                              >  8:325434/1‑132 (MQ=255)
                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTcc                                                                                              <  7:325434/132‑1 (MQ=255)
                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAg                                                                                       >  6:245221/1‑139 (MQ=255)
                                             tGACACTGATGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCACTGCAAg                                                                                       >  5:217126/1‑139 (MQ=255)
                                                 actgttGGGCGCAGTCATAAAGTTAATCCCCGTTACGCTCGGCAATCTCACCGCGGGAATAGTTATCAGGGGCTGGATGTACAACACACTGAATCCTGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCaa                                                                                   <  4:28646/134‑1 (MQ=255)
                                                 actgaTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCa                                                                                    >  2:326880/5‑138 (MQ=255)
                                                     aTGGGAGCAGTCAGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGATTAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCg                                                                               >  3:209976/1‑139 (MQ=255)
                                                                 aGAAACTTAATCCCCGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATACAAACACTTCCAGAGATCACTCCCTGCCAGGCAAAGCGGTTATTCCga                                                                     >  5:216478/1‑137 (MQ=255)
                                                                          agacccGTTACGCTCGGAATACTTCCCGCGGTAATATTTAGTATGGGCTTCATTAAATCCACACTGAATCCTCAGCAAAAAAAAAACTTCCACAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGaa                                                          <  6:217126/136‑1 (MQ=255)
                                                                               cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGa                                                           <  6:89882/133‑1 (MQ=255)
                                                                               cGTTACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGa                                                           >  5:89882/1‑133 (MQ=255)
                                                                                 ttACGCTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTg                                                   >  1:149901/1‑139 (MQ=255)
                                                                                 ttACGCTCGGCAATCTGACAGAGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCATTATCAATAAAAAAACTTCCAGAGATAACTCCCTGCAAGTCAAAGCGGTTATTCCTACAGATCTTGAAGACGCTg                                                   >  5:264352/1‑139 (MQ=255)
                                                                                     gcTCGCCAATCTGACCCGGGGAATCGTTATTATGGGCTGGATGAACTACACACTGAATCCTGATCAAAAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACa                                               <  1:330610/138‑1 (MQ=255)
                                                                                      cTCGGCAATCTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACAt                                              >  5:296547/1‑139 (MQ=255)
                                                                                             atcTGACCGCGGGAATAGTTATGATGGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGt                                       >  1:290338/3‑139 (MQ=255)
                                                                                                                 atgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                          <  7:103376/116‑1 (MQ=255)
                                                                                                                 atgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAg                                          >  8:103376/1‑116 (MQ=255)
                                                                                                                 atgatgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGatt                   >  8:239183/1‑137 (MQ=255)
                                                                                                                    atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCt                         <  5:305640/130‑1 (MQ=255)
                                                                                                                    atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCt                         >  6:305640/1‑130 (MQ=255)
                                                                                                                    atgGGCTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCg                >  6:188965/1‑139 (MQ=255)
                                                                                                                         cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGCTCACTCCCTGCAAGTCACAGCGGTTATTCAGCGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa           >  8:143016/1‑139 (MQ=255)
                                                                                                                         cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                             <  4:96276/73‑1 (MQ=255)
                                                                                                                         cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGt                                                                             >  3:96276/1‑73 (MQ=255)
                                                                                                                         cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa           >  5:305339/1‑139 (MQ=255)
                                                                                                                         cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa           >  2:86475/1‑139 (MQ=255)
                                                                                                                         cTGGATGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCaaa           >  4:244464/1‑139 (MQ=255)
                                                                                                                              tGTACTACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTaa      >  2:134784/1‑139 (MQ=255)
                                                                                                                                  ctACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGa  >  3:19404/2‑139 (MQ=255)
                                                                                                                                   tACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGa  >  6:325907/1‑138 (MQ=255)
                                                                                                                                   tACACACTGAATCCTGATCAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGATTCCGGCAAATGTAATTGa  >  7:150292/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGTGCACATTCGCCATCTCACTTTTGATCGAGCACCCTGATACAGTGACAGTAATGGGAGCAGTCAGAAACTTAATTCCCGTTACACTCGGCAACCTGACCGCGGGAATGGTCATGATGGGCTGGATGTATTACACGCTCAATCCCGATAAATAAAAAAACTTCCAGAGATCACTCCCTGCAAGTCAAAGCGGTTATTCCGAGAGATCTTGAAGACGCTGGACATGCAGCGTGATATAGCCGGCCTCGGACTCCGGCAAATGTAATTGA  >  CP006881/3723803‑3724071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑