Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,723,022	G→A	intergenic (‑136/‑165)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,022	0	G	A	100.0%	207.6 / NA	64	intergenic (‑136/‑165)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/37);  total (27/37)

CTGTTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATG  >  CP006881/3722887‑3723156                                                                                                                                        |                                                                                                                                        cTGTTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTGattaagattaag                                                                                                                                       >  8:3174/1‑138 (MQ=255)         cccccTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGTACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCtt                                                                                                                              >  6:211341/1‑139 (MQ=255)          ccccTTATATTTAAACAAAATAGACCTAAAATTCTCCAACGAATTAAGGACTCAGCGATCCTTTTTCATTTACTCAAATTTTAGGTCTTGCCTGCTTTACCAGTCTCAATCCCGGTTATTCAGATTAAGAATACGCttt                                                                                                                             <  5:304370/139‑1 (MQ=255)              ttttATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTtcat                                                                                                                          >  2:93635/1‑138 (MQ=255)                ttctttaTACCAAAAAGACCTAAAATTCGCCAACTAATAAATGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTAATCAGATTAAGAATACGCTTtcatca                                                                                                                        <  1:197425/135‑1 (MQ=255)                              aaGACCTAAAATTAGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCAAAAATCATGATAGCg                                                                                                         <  5:211341/139‑1 (MQ=255)                                          tCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAc                                                                                              >  5:90516/1‑138 (MQ=255)                                            gCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACtat                                                                                           >  3:124821/1‑137 (MQ=255)                                                 cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttt                                                                                     <  4:237762/139‑1 (MQ=255)                                                 cGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACT‑Atttttt                                                                                     <  5:114016/139‑1 (MQ=255)                                                         ggACACATCGATACTTTTTCATTTAAGCAAATTTTAGGTCTTGCCTGCTTCACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTaaa                                                                              <  2:154645/139‑1 (MQ=255)                                                         ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTaaa                                                                              <  7:103614/139‑1 (MQ=255)                                                         ggACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGGGACAATCCCGCTTAGTCAGATTAAGAATACGCTTTCATCATAACTCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTaaa                                                                              <  7:256459/139‑1 (MQ=255)                                                            cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAg                                                                           >  6:311533/1‑139 (MQ=255)                                                            cacaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTAGTCAACTATTTTTTTAGTTAAATAg                                                                           >  7:52860/1‑139 (MQ=255)                                                             acaGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGt                                                                          >  7:349649/1‑139 (MQ=255)                                                              caGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGtt                                                                         <  7:292196/139‑1 (MQ=255)                                                               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGttt                                                                        >  1:331774/1‑139 (MQ=255)                                                               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGttt                                                                        >  3:8445/1‑139 (MQ=255)                                                               aGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTCACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGATTTCGTCAACTATTTTTTTAGTTAAATAGttt                                                                        >  1:154645/1‑139 (MQ=255)                                                                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATa                                                                    >  2:119083/1‑138 (MQ=255)                                                                    tCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATa                                                                    >  1:284771/1‑138 (MQ=255)                                                                         tttCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAAcat                                                              >  2:190057/1‑137 (MQ=255)                                                                                                 ttGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTaaaaatt                                       >  4:192760/1‑136 (MQ=255)                                                                                                  tGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTaa                                     >  6:156284/1‑138 (MQ=255)                                                                                                   gCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAATATTTTTTTAGTTAAATAGTTTGATATAACATTTAGACAAAAATTCGTAAAAATTaat                                    >  4:4392/1‑137 (MQ=255)                                                                                                        cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                <  5:311533/138‑1 (MQ=255)                                                                                                        cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgtg                                >  6:153615/1‑138 (MQ=255)                                                                                                           tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                             <  6:331572/138‑1 (MQ=255)                                                                                                           tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaa                             <  8:107592/138‑1 (MQ=255)                                                                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATACATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  4:302169/138‑3 (MQ=255)                                                                                                             ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaata                           <  1:93635/138‑3 (MQ=255)                                                                                                                 tCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                      <  7:40396/139‑1 (MQ=255)                                                                                                                  cacaATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTCTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                      >  5:262224/1‑138 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  7:3174/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  5:218801/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:349649/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  5:156284/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  5:153615/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  2:145011/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  2:284771/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  3:192760/139‑1 (MQ=255)                                                                                                                      aTCCCGCTTAATCAGATTAAGAATACGCTTTCATCATAAATCCTGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  6:143918/139‑1 (MQ=255)                                                                                                                      aTCCCGCATATTCAGATTAAGAATACGCTTTCCTCATAAATCATGATAGCGGTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATCTAACATGTAGACAAAAAATCGTAAAAATTAATTGTGAAATACTTCACAata                 <  8:64989/139‑1 (MQ=255)                                                                                                                         ccGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTCTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc               <  6:262224/138‑1 (MQ=255)                                                                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  4:8445/139‑1 (MQ=255)                                                                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            >  1:259860/1‑139 (MQ=255)                                                                                                                           gCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg            <  2:331774/139‑1 (MQ=255)                                                                                                                            cTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGc           >  6:314124/1‑139 (MQ=255)                                                                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt          <  5:139553/137‑1 (MQ=255)                                                                                                                               aTTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCt          <  6:103189/137‑1 (MQ=255)                                                                                                                                ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtata       <  1:119083/139‑1 (MQ=255)                                                                                                                                 tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATTTAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  1:327447/128‑1 (MQ=255)                                                                                                                                 tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATTTAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 >  2:327447/1‑128 (MQ=255)                                                                                                                                 tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 >  4:285118/1‑128 (MQ=255)                                                                                                                                 tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  3:285118/128‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 >  3:208643/1‑124 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 >  1:145258/1‑124 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 >  1:80360/1‑124 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  2:145258/124‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  2:80360/124‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  4:208643/124‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg  >  2:64805/1‑139 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCATCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                 <  6:80417/124‑1 (MQ=255)                                                                                                                                     aTTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCATCAACTATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTAACAata                 >  5:80417/1‑124 (MQ=255)                                                                                                                                        |                                                                                                                                        CTGTTTCTCCCCCTTTTATTTAAACAAAAAAGACCTAAAATTCGCCAACGAATAAAGGACACAGCGATCCTTTTTCATTTACGCAAATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAACT‑GTTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATG  >  CP006881/3722887‑3723156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑