| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,725,209 | +T | intergenic (‑41/‑488) | U712_19205 ← / → U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,725,205 | 1 | . | T | 83.0% | 124.3 / 21.7 | 51 | intergenic (‑37/‑492) | U712_19205/U712_19210 | Uncharacterized protein ywcI/Minor extracellular protease vpr |
| Reads supporting (aligned to +/- strand): ref base . (3/5); new base T (28/11); total (35/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑C‑TT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATAT > CP006881/3725068‑3725337 | tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCGTTTTGGCAGGCCG‑C‑C‑t > 1:183024/1‑139 (MQ=255) gAGCCGAATAGACCGCGGCAGCCGCGGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCG‑C‑C‑tt > 8:282206/1‑139 (MQ=255) gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCGTCTTTTTTGCAGGCAG‑C‑C‑tt > 1:366287/1‑139 (MQ=255) gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACACAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACTTTT‑CATATAAACAAAGCCCTCCGCGTCTTTTTGTCAGGCAG‑C‑C‑tt > 4:359149/1‑139 (MQ=255) gAGCCGAATAGACCGCGGCAGCAGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGAGACAAGCAAACTTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCAG‑C‑C‑ta > 7:430080/1‑138 (MQ=255) accgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGccc > 8:46607/4‑139 (MQ=255) ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCCG‑C‑C‑TTGTTA‑GCAGCCCGtgt > 3:8433/1‑136 (MQ=255) ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGtttt > 8:275588/1‑139 (MQ=255) ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGtttt > 3:218627/1‑139 (MQ=255) ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGtttt > 3:172803/1‑139 (MQ=255) ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCAACAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAGACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CGTT‑TTA‑GCAGCCCGtttt > 2:156575/1‑139 (MQ=255) gTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTTGCAGGCAG‑C‑CTTT‑TTA‑TCAGCCCGTTTTCTCAGCCGCa > 8:307621/1‑139 (MQ=255) ttCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGcc < 7:370443/138‑1 (MQ=255) cTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCTCGTTTTCTCAGCCGCAGcccg < 7:65576/138‑2 (MQ=255) cTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGcccg < 2:43838/138‑2 (MQ=255) tCCGGCGTCCCCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAACCAAACGTTT‑CATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCAG‑C‑CATT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGccagcaa > 5:231880/1‑133 (MQ=255) cGGCGTCCCCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCGTCTTTTTTGCCGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGcccgcaaga > 5:100043/1‑132 (MQ=255) cGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCATTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTAAGCCGCAGcccgcaaga > 8:39020/1‑132 (MQ=255) cccAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GGAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGc > 5:192803/1‑139 (MQ=255) cccAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGc > 5:199749/1‑139 (MQ=255) gTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CATT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAt > 1:299404/1‑139 (MQ=255) ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtt > 8:19855/1‑139 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCCGCCCGCAAGAAAAGCCGGCCGAgttctgt > 4:26443/1‑137 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAgttcttt > 1:405840/1‑139 (MQ=255) caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGGCAG‑C‑CGTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAgttattt > 1:383664/1‑139 (MQ=255) aTCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAc > 4:422918/1‑139 (MQ=255) cccGTCAAGAAACACCCAACCTTTT‑CATACAAAAACATCCCTCGGCTTGTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTACTCAGCCGCAGCCCTCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGc < 7:39020/139‑1 (MQ=255) cccGAAAACAAAAAACCAAACGTTC‑CAAACAAAAACATCCCGCGGCTTCTTTTTGGCCGCCAG‑C‑A‑TT‑TTACGCCCCCCTTTTTCTCAGCCGCCCCCCGAAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGc < 7:402937/139‑1 (MQ=255) tAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACAAGCGGctc > 2:355377/1‑137 (MQ=255) aGATACATCAAAACGTTT‑AAAAAAAAAACACCCCTCCGCTTCTTTTTGGCCGGCCG‑C‑CTTT‑TTC‑GCAGCCCTTTTTCTCCCCCCCCGCCCGCACAAAATCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa < 4:218627/139‑1 (MQ=255) aCAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCAG‑C‑CTTT‑TTT‑GCCGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGCCGGCCGATTTGTTTTGTCGCCAACTCGCGGCTCTATCaaa > 6:33227/1‑137 (MQ=255) aCAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGCTTTCTTTTCTCGCAACCGAGCGGCTCTAGCAAAAc > 5:373337/1‑139 (MQ=255) aCAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc > 8:103549/1‑139 (MQ=255) aCAAGCAAACGTAT‑CATAGAAAAACATCCCTCCGCTTGTTTTAGGCAGGCCG‑C‑CTTT‑TTT‑GCAGCCCGTTTTCTCCCCCGCAGCCCGCAAGAAAAGACAGCCCAATAGGTGTCTCGCAAACTAGCGGCTGGACCaaaa > 4:189834/1‑138 (MQ=255) aaGCAAACGTTT‑CATAGAAAAACATCCCTCCGCGTCTTTTTTGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCCGCCGCAGCCCGCAAGAAAAGACGGCCCAGTTCGTTTCTCGCAAACAAGCGGCTCGACCaaacag > 1:85066/1‑135 (MQ=255) gaaaaCGTTT‑CATATAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑CAT‑TT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCCCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:307621/137‑1 (MQ=255) gCAACCGTTT‑CATAGAAAAACCTCCCTCCGCTTCTTTTTGGCCGGCAG‑C‑CTTT‑TTA‑GCACCCCGTTTGCTCAGCCGCCGCCCGCAACAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 8:386712/139‑1 (MQ=255) gCAAACTTTT‑AATACAAAAACACCCCTCCGCTTTTTTTGGGCCGGCAG‑C‑C‑TT‑TTACCCCCCCCGTTTTCTCCCCCCCCGCCCCCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 1:355377/139‑1 (MQ=255) gCAAACGTTT‑CATATAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCCGCCGGTTTTCTCACCCGCAGCCCGCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 3:422918/139‑1 (MQ=255) gCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:275588/139‑1 (MQ=255) gCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAGTTATTTTCTCGCAAACTAGCGGCTCTAGCCAAACTCAt > 6:68866/1‑139 (MQ=255) ccAAACGTTT‑CATACAAAAACATCCCGCCGCTTCTTTTTGGCCGCCAG‑CAT‑TT‑TTC‑CCACCCCGTTTTCTCAGCCCCCCCCCCAAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 7:46607/138‑1 (MQ=255) ccAAACGTGT‑CAAACAAAACCATCCCTCGGGTACTTTTGGCGCGGCAG‑C‑CTTT‑TTA‑GCACCCGGTTTTCTCCGCCGCTGCCCGCCAGAAAAGACGCCCGATTTATTTTCTCGCAAACAATCGGCTCTAGCAAAACTCAt < 6:159651/138‑1 (MQ=255) caaaaCGTTA‑AATATAAAAACATCCCTCCGCTTCTTTTTGGCAGCCAG‑C‑CTTT‑TTC‑CCAGCCCGTTTTCACCGCCGCAGCCCGAAATAAAACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt < 2:299404/137‑1 (MQ=255) ccaaCGTTTACAAACAAAAACACCCCTCCGATTTTTTTTGGCGCGCCC‑C‑CTTT‑TTC‑CCCGCCTGTTTACTCCGCCCCACCCCCCAACAAAACACGGCCGATTTCTTTTCTCGCAAACAAGCGGCTCTAGCAAAACTCAt < 6:429304/137‑1 (MQ=255) aCGTTT‑CATAGAAGAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAcg > 2:381336/1‑138 (MQ=255) aCGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTAGCAAACTAGCGGCTCTAGCAAAACTCATGAct > 5:429304/1‑139 (MQ=255) aCGTTT‑AATACAAAAACACCCCCCCGCTTCTTTTTGCCTGCCAG‑CTT‑TT‑TTA‑CCACCCGGTTTTCACCGCCCCAGCCCCAAACAAACGCCGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct < 7:282206/139‑1 (MQ=255) cGTTT‑CATAGAAAAACATCCATCCGCTTATTTTTGGCAGGCAGAC‑T‑TT‑TTA‑GCAGACCGTTTTCTCAGCCGCAGCCCGCAAGAAAATACGGCCGATTTGTTTTCTCCCAAAATAGAGGGTCGAGCAAAACTCATTActc > 1:139961/1‑139 (MQ=255) t‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATTACTCTAt > 2:286095/1‑138 (MQ=255) cATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTAATGACTCTATGa > 3:98079/1‑139 (MQ=255) aaaCATCCCTCCGCTTCTTTTTGGCAGGCAGAC‑T‑TT‑TTA‑GCAGCCCGATTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTATTTTCTAGCAAACTAGCGGCTCTAGCAAAACTCATGACTCAAAGATAGTCCg > 8:446484/1‑139 (MQ=255) ttttGGCAGGCCG‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTGTTTTATCGCAAACTAGCGGCGCGCGCAAAACTCATGACTCGATGATAGACCGTGGAAAGAGAATTGtttt > 2:229296/1‑138 (MQ=255) tttGGCAGGCAG‑C‑CTTT‑TTA‑GCCGCCCGTTTTATCAGCCGCAGCCCGCCAGAAAAGACGGCCGATTTAGTTTATCGCCAACAAACGGCGCTAGCAAAACACAATCCTCTATTAAAGACCCTGGAAAGACAAttaatttt > 4:421995/1‑133 (MQ=255) gcag‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAatat > 5:170270/1‑139 (MQ=255) gcag‑C‑CTTT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAatat > 3:107167/1‑139 (MQ=255) | TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTT‑CATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAG‑C‑C‑TT‑TTA‑GCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATAT > CP006881/3725068‑3725337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |