Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,724,980	T→C	K63K (AAA→AAG)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,724,980	0	T	C	100.0%	117.7 / NA	33	K63K (AAA→AAG)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/29);  total (4/29)

CTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCG  >  CP006881/3724858‑3725110                                                                                                                           |                                                                                                                                   cTGGGTTTTTACCTCCTTTTGAGCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                                                                                                    <  6:118670/139‑1 (MQ=255) cTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca                                                                                                                    <  2:139475/139‑1 (MQ=255)               cctTTTGACCGCCTTGTAAAGATGCGGGACCGCTCTCTCCTGCTTTGGAGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGATGCTTTCGCTTTCTGCTTTTCTTACTCATTCACAGCCATCCGcc                                                                                                        <  2:218250/137‑1 (MQ=255)                    tGAGCGCGATGTATTGTTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCAt                                                                                                  <  1:124938/138‑1 (MQ=255)                             tGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAAc                                                                                        <  1:291701/139‑1 (MQ=255)                                 aaGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAg                                                                                     <  3:119430/138‑1 (MQ=255)                                    aTGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTcct                                                                                 <  1:167643/139‑2 (MQ=255)                                                    tcCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTACTCATTCACAGCCATCCGCCATTCATAAAAAGAAAAGAGTCCTTCGGCCTGGAccgccc                                                                 >  3:129385/1‑138 (MQ=255)                                                      cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACACCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagccg                                                                 <  1:405510/137‑1 (MQ=255)                                                         cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTcc                                                                                                                           >  3:146395/1‑76 (MQ=255)                                                         cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTcc                                                                                                                           <  4:146395/76‑1 (MQ=255)                                                            tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                          <  3:104161/138‑1 (MQ=255)                                                            tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                          <  5:99033/138‑1 (MQ=255)                                                             ggCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCtt                                                         <  1:206375/138‑1 (MQ=255)                                                              gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCAt                                                                                                  <  2:252566/96‑1 (MQ=255)                                                              gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCAt                                                                                                  >  1:252566/1‑96 (MQ=255)                                                              gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                        <  8:58207/138‑1 (MQ=255)                                                              gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                        <  2:60284/138‑1 (MQ=255)                                                                  ttCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                   <  6:137806/139‑1 (MQ=255)                                                                  ttCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                   <  4:312710/139‑1 (MQ=255)                                                                         agggCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                            <  3:182018/137‑1 (MQ=255)                                                                         aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                            <  3:138636/139‑1 (MQ=255)                                                                         aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                            <  8:109840/139‑1 (MQ=255)                                                                            gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                         <  3:444528/139‑1 (MQ=255)                                                                            gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                         <  6:138853/139‑1 (MQ=255)                                                                            gCTGGCAAACATCTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                         <  2:161536/139‑1 (MQ=255)                                                                               tGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCTTTCACAGCCATCCGCCATTCATAAAACGAAACGGGTCCTGCGGCCTGCAcagccg                                                                 >  2:48467/1‑112 (MQ=255)                                                                               tGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCTTTCACAGCCATCCGCCATTCATAAAACGAAACGCGTCCTGCGGCCTGCAcagccg                                                                 <  1:48467/112‑1 (MQ=255)                                                                                           cttTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAgctgtc                            <  5:113991/137‑6 (MQ=255)                                                                                                              cttaggCTTTATGCTTTTCTCCCTAATTCACACCCATCCGCCATGCATAAATCGAACCGAGTACTGCGCCCTGCACAGCCGCCTTTCTTTGGTAGGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc       <  6:305360/134‑1 (MQ=255)                                                                                                                  cGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAACCGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCgg    <  3:150074/138‑1 (MQ=255)                                                                                                                   gCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg  <  6:138671/139‑1 (MQ=255)                                                                                                                   gCTTTCTGCTTTTCTTCCTCATACACAGGCATCCGCCTTTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGGCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCg  <  1:218843/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                   CTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCG  >  CP006881/3724858‑3725110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑