| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,801,996 | G→A | A327T (GCC→ACC) | U712_14440 → | UPF0118 membrane protein ytvI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,801,996 | 0 | G | A | 95.5% | 207.2 / ‑6.0 | 68 | A327T (GCC→ACC) | U712_14440 | UPF0118 membrane protein ytvI |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (26/38); minor base T (1/2); total (28/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATAT > CP006881/2801861‑2802114 | ttATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTGTTATTAAGCGGCAGCTGTCAGAGCCCAAGATACTAAGTAAATCTATCGGGAGAGATCCGCTGGGCACCCGCATCAccc > 1:228285/1‑139 (MQ=255) ttACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc < 3:104138/139‑1 (MQ=255) ccAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATGACCCTGTTTGCCGGCTTTAAATTATTc < 5:440643/139‑1 (MQ=255) ccAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTc < 1:302533/139‑1 (MQ=255) aGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 2:30594/138‑1 (MQ=255) aGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg > 1:30594/1‑138 (MQ=255) ttGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACt < 6:272741/139‑1 (MQ=255) aTCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATAATAAGTAAAACAATCGGGGTCGATCCCCTGGCAACACGCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTTGGACTTATTg > 4:221711/1‑139 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGa < 2:274891/138‑1 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc > 5:110019/1‑139 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc < 8:93233/139‑1 (MQ=255) tACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGAc < 2:24543/139‑1 (MQ=255) cgtcgtTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCt > 7:402192/1‑139 (MQ=255) cgtcgtTCTTATTAAGCGGCAGCTGACAGAGCCCAAAATACTAAGTAAATGTATCCGGATGCATACGCTGGCAACACTCATCACCCTGTTTGGCGGCTTTAAATTTTAGGTGTTTTTAGGTCAGATTTCGGGGACGGCt > 4:216494/1‑139 (MQ=255) cgtcgtTCTTATTAAGCGGCAGCGGACAGAGCCCAAGATACTAAGTAAATATATCGGGATCGAAACGCTGGCAACACTCAACACCCGGTTTGCCGGCGTTTAATTATTATGGTTTTTTTGACGGATTGAGGGACCGGCt > 4:157319/1‑139 (MQ=255) cgtcgtGCTTATTAAGCGGCCGCTGACAGAGCCCAAGATACTAAGTAAATATATCGGGATCGCTCCGCGGGCAACACTCATCACCCGGTTTGGCGGCTTTAAATTTTTGGGGTTTTTTGGACGGATTTCGGGGCCGGCt > 8:392697/1‑139 (MQ=255) tcgtTCTTATTAAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATACACTGGCAACACTCATCACCCTGTTTGCCGGCGTTAAATTATTAGGGTTTTTAGGTCTGATTGCGGGACCGGCTGt > 8:11303/1‑139 (MQ=255) cTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTc < 5:316629/138‑1 (MQ=255) aTTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtc < 1:416653/138‑1 (MQ=255) ttCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtca < 6:110019/138‑1 (MQ=255) cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATcc < 3:157319/139‑1 (MQ=255) cGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATcc < 2:366878/139‑1 (MQ=255) cAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGCCCGGCTGTACTCGTCATCATCCAAGCTTtcat < 8:191000/139‑1 (MQ=255) agCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 3:183290/82‑1 (MQ=255) agCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg > 4:183290/1‑82 (MQ=255) agCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTCGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTtcatca > 8:329189/1‑138 (MQ=255) aGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtca < 5:136711/114‑1 (MQ=255) aGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtca > 6:136711/1‑114 (MQ=255) aGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAgg > 2:58451/1‑139 (MQ=255) tAAGTAAATCTATCTGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAtt < 1:389086/139‑1 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt > 6:442647/1‑138 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtca > 7:144840/1‑108 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGtca < 8:144840/108‑1 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACACCAGGCGCAt > 6:414036/1‑138 (MQ=255) tAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCAt > 8:141999/1‑138 (MQ=255) aGTAACTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa < 1:273250/139‑1 (MQ=255) aGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa < 2:228285/139‑1 (MQ=255) aGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa < 3:34651/139‑1 (MQ=255) aGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTa > 4:34651/1‑138 (MQ=255) gTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaaa < 8:23635/139‑1 (MQ=255) aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc > 8:320185/1‑45 (MQ=255) aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGc < 7:320185/45‑1 (MQ=255) aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCACGCTTTCATCACAACAGGCGCATTAAAAGa > 6:87377/1‑138 (MQ=255) aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa < 3:409455/134‑1 (MQ=255) aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTaa > 4:409455/1‑134 (MQ=255) aTCTATCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGa > 7:38995/1‑138 (MQ=255) tCGTGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCCCCTTTAAATTATTCGTGTTATTAGGACTGATAGCGGGACCGGCTGAACTCGTCAGCATCCAAGCATTAATCACAACAGGCGCAATAAAACAAATATg > 5:297055/1‑139 (MQ=255) tCGGGATCGATCCGCTGGCGACACTCCTCTCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCAGCATCCAAGCTTTCATCACAACAGCCGCATTAAAAGAAATATg > 4:425859/1‑139 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCt > 8:420453/1‑107 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCt < 7:420453/107‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 7:329189/137‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 7:392697/137‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 8:38995/137‑1 (MQ=255) tCGGGATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 8:120256/137‑1 (MQ=255) tCGGGATCGATCCACTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAata < 7:11303/137‑1 (MQ=255) gggTTCGATACGCAGGCTACACTCATCTCCCTGTTTTCCGGCTTTAATTTATTCGGGTTTTTAGGTCTGATTGCGGGACCGGCTGTACTCGGCATCATCCAAGCTTTCATGACTACAGGCGCATTAAAAGAAATATgg < 3:224411/138‑1 (MQ=255) gggATCGATCCGCTGGCAACACTCATCTCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCGGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg < 5:442647/138‑1 (MQ=255) gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg < 6:259187/138‑1 (MQ=255) gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg < 5:30722/138‑1 (MQ=255) gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg < 7:141999/138‑1 (MQ=255) gggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGAGTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATgg < 3:43480/138‑1 (MQ=255) ggATCGATCCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTc < 8:402192/139‑1 (MQ=255) tcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg < 3:316515/52‑1 (MQ=255) tcgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCgg > 4:316515/1‑52 (MQ=255) cgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTc < 4:70381/135‑1 (MQ=255) cgatcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTc > 3:70381/1‑135 (MQ=255) atgcGCTGGCAACACTCATCACCCTGTTTGCCGTCTTTTAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACATGCGCATTAAAAGAAATATGGTCCTatat < 3:297156/136‑1 (MQ=255) tcCGCTGGCAACACTCATCACCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGCCTCATTAAAAGAAATATGGTCCTatat > 4:297156/1‑138 (MQ=255) | TTATCGATTACGGGCCAGCTGCCCCAAGCAATCGGAATTGGTATCCTGTACCTCGTCGTTCTTATTCAGCGGCAGCTGACAGAGCCCAAGATACTAAGTAAATCTATCGGGATCGATCCGCTGGCAACACTCATCGCCCTGTTTGCCGGCTTTAAATTATTCGGGTTTTTAGGACTGATTGCGGGACCGGCTGTACTCGTCATCATCCAAGCTTTCATCACAACAGGCGCATTAAAAGAAATATGGTCCTATAT > CP006881/2801861‑2802114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |