Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,596,615 G→A A167V (GCT→GTT)  U712_18505 ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,596,6150GA98.1% 167.9 / ‑3.8 54A167V (GCT→GTT) U712_18505UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase 1
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (30/22);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

TGCCAGCTCCGCGGATTTTTCCGCCCATGCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAGCTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATCAAT  >  CP006881/3596481‑3596745
                                                                                                                                      |                                                                                                                                  
tgccAGCTCCGCGGATTTTTCCGCCCATGCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTcc                                                                                                                                <  1:339133/139‑1 (MQ=255)
  ccAGCTCCGCGGATTTTTCCGCCCATGCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTa                                                                                                                              <  2:19235/139‑1 (MQ=255)
  ccAGCTCCGCGGAGTTTTCCGCCCATGCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTa                                                                                                                              <  5:85381/139‑1 (MQ=255)
    aGCTCCGCGGATTTTTACGCCCATGCCGTTGATATAGTTTTATAAATAAACGATTTCTGGTTGATTTGCCACGTTTTACATCGTTGTTGTTACTTCAGCGAGCGCCGCTGGCATAAAAAGGTTCTAGGTAACTCCTacc                                                                                                                            >  6:56727/1‑138 (MQ=255)
         cgcgGATTTTTCCGCCCATGCCGTTGTTATAGTTTGCTAAATCAACGCTTTCGGGTTCTTTTGGCCCGTTTTGCAGCGGTGTTGTTGCTTCAGCTAGAGCGGCTGGCATTATCAGGTTCGCTGTAACTCCTCCACTTgg                                                                                                                       >  6:31988/1‑139 (MQ=255)
         cgcgGATTTTTCCGCCCATGCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTA‑CTCCTACACTTggg                                                                                                                      <  6:461141/139‑1 (MQ=255)
                ttttCCGCCCATGCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTGCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCCTAATCAGGATCTCTGTAACTCCTACCCTTGGGAAGTc                                                                                                                 >  6:201815/1‑138 (MQ=255)
                 tttCCGCCCATGCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTcc                                                                                                                <  4:199557/138‑1 (MQ=255)
                            gCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAAttttt                                                                                                    >  4:198908/1‑139 (MQ=255)
                            gCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCAACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAAttttt                                                                                                    >  1:265787/1‑139 (MQ=255)
                                  gATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGcc                                                                                               <  3:97586/138‑1 (MQ=255)
                                   atatAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCt                                                                                              <  5:439720/138‑1 (MQ=255)
                                        gTTTGCTAACTCAACGATTTCGGGTTCTTTTGCCACGGTTTCCAGCGGTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAAATTTTGCGCCTTGCAGt                                                                                        <  5:118448/139‑1 (MQ=255)
                                        gTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGt                                                                                        >  4:287657/1‑139 (MQ=255)
                                        gTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGt                                                                                        <  3:55078/139‑1 (MQ=255)
                                        gTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTCTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGt                                                                                        <  4:271874/139‑1 (MQ=255)
                                         tttGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTc                                                                                       >  1:196732/1‑139 (MQ=255)
                                          ttGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCg                                                                                      >  1:30330/1‑139 (MQ=255)
                                           tGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGAGAAATTTTTGCGACTATCAGTCgg                                                                                     >  2:179319/1‑139 (MQ=255)
                                            gCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAGCTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCgg                                                                                     <  5:344500/138‑1 (MQ=255)
                                             cTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCATTCAGCTAGAGCGGCTGCCATACTCAGGTTCTCTGTAACTCCTACACTAGGGAAGTCCAGATAAATTTTTGCGCCTTTCAGTCGGcc                                                                                   >  7:180215/1‑139 (MQ=255)
                                                          ttCGGGGTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGc                                                                       <  4:266367/138‑1 (MQ=255)
                                                           tCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCt                                                                      <  3:287657/138‑1 (MQ=255)
                                                                ttCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATg                                                                <  2:243053/139‑1 (MQ=255)
                                                                 tCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGa                                                               <  7:379112/139‑1 (MQ=255)
                                                                               ttCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGAc                                                  <  1:145573/138‑1 (MQ=255)
                                                                                         gttgttCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCt                                        <  3:198908/138‑1 (MQ=255)
                                                                                         gttgttCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTg                                       >  8:410760/1‑139 (MQ=255)
                                                                                               ccTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCATCTTCAATGAAGCCATTACCGCCTTTGATTTCATCGCCCa                                 >  7:113751/1‑139 (MQ=255)
                                                                                               ccTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCa                                 >  6:443648/1‑139 (MQ=255)
                                                                                                  tCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTcc                                                                                                                                >  5:65144/1‑41 (MQ=255)
                                                                                                  tCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTcc                                                                                                                                <  6:65144/41‑1 (MQ=255)
                                                                                                  tCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTg                              >  3:103047/1‑139 (MQ=255)
                                                                                                  tCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTAAGCTTCAATGAAGCCATTTCCGACTTTGATTTCTGCGCACATTg                              >  8:172948/1‑139 (MQ=255)
                                                                                                         gagCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCaa                        <  5:443648/138‑1 (MQ=255)
                                                                                                         gagCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCGTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCCTTGCTTCaa                        >  5:64007/1‑138 (MQ=255)
                                                                                                          agCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCaaaa                      >  3:62026/1‑139 (MQ=255)
                                                                                                           gCGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAAc                     <  4:346749/139‑1 (MQ=255)
                                                                                                            cGGCTGCCATAATCAGGTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAAcc                    >  7:164813/1‑139 (MQ=255)
                                                                                                              gCTGCCATAATCAGGTTCTCTGTAACTACAACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCATTTAATTAAGCTTCAAAGAATCCAATTACGACATTGATTTATTCGCCCAATGCTAGAAAAACta                  >  6:289789/1‑138 (MQ=255)
                                                                                                               cTGCCATAATCAGGTTCTCTTTAACTACTACAATTTGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCATATTCAATGAAGCCAAAAACGCCTATTATTTATTCGCCCAATGTGTAAAAACCttt                 >  8:405900/1‑139 (MQ=255)
                                                                                                                            gTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATc     >  2:278542/1‑138 (MQ=255)
                                                                                                                            gTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATc     >  7:269842/1‑138 (MQ=255)
                                                                                                                            gTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATc     >  1:394528/1‑138 (MQ=255)
                                                                                                                            gTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATc     >  6:83767/1‑138 (MQ=255)
                                                                                                                            gTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATc     >  3:171859/1‑138 (MQ=255)
                                                                                                                            gTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATc     >  3:33627/1‑138 (MQ=255)
                                                                                                                            gTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCAGTACCGACGTTGATTTCTGCGCCCATTGCTTCACAACCTTTTAAATGCTGATc     >  4:219054/1‑138 (MQ=255)
                                                                                                                            gTTCTCTGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTAAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATc     >  1:169129/1‑138 (MQ=255)
                                                                                                                               ctctGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATCAAt  <  2:265787/138‑1 (MQ=255)
                                                                                                                               ctctGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATCAAt  <  1:198236/138‑1 (MQ=255)
                                                                                                                               ctctGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATCAAt  <  8:164813/138‑1 (MQ=255)
                                                                                                                                tctGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTAc                                                      <  3:126902/85‑1 (MQ=255)
                                                                                                                                tctGTAACTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTAc                                                      >  4:126902/1‑85 (MQ=255)
                                                                                                                                      |                                                                                                                                  
TGCCAGCTCCGCGGATTTTTCCGCCCATGCCGTTGATATAGTTTGCTAAATCAACGATTTCGGGTTCTTTTGCCACGTTTTCCAGCGTTGTTGTTCCTTCAGCTAGAGCGGCTGCCATAATCAGGTTCTCTGTAGCTCCTACACTTGGGAAGTCCAGATAAATTTTTGCGCCTTGCAGTCGGCCTTTTACTTCAGCTTCAATGAAGCCATTACCGACTTTGATTTCTGCGCCCATTGCTTCAAAACCTTTTAAATGCTGATCAAT  >  CP006881/3596481‑3596745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑