Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,578,472	(A)7→6	intergenic (‑65/‑70)	U712_18365 ← / → U712_18370	Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,578,466	0	A	.	100.0%	212.7 / NA	47	intergenic (‑59/‑76)	U712_18365/U712_18370	Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (15/32);  total (15/32)

TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGATTTA  >  CP006881/3578329‑3578600                                                                                                                                          |                                                                                                                                       ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACCACCTATCCa                                                                                                                                        >  6:121594/1‑138 (MQ=255) ttGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCa                                                                                                                                        >  7:173013/1‑138 (MQ=255)         aaCACCCAGAGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCaaaaaacttt                                                                                                                               <  1:42380/139‑5 (MQ=255)            acCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACttttt                                                                                                                            >  8:307662/1‑138 (MQ=255)                aGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGAt                                                                                                                       <  4:308354/139‑1 (MQ=255)                  cGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATg                                                                                                                      >  5:278298/1‑138 (MQ=255)                   gTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTGTTTTGTTGCTCGCTCATTGTTTTATCCCCAGAGCCACATTCTGAACACTTTTATGTAAATTTATACAACCTATCC‑AAAAAACTTTTTTTGATGtt                                                                                                                    >  4:186945/1‑138 (MQ=255)                   gTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATg                                                                                                                      >  6:321464/1‑137 (MQ=255)                   gTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATg                                                                                                                      >  7:96800/1‑137 (MQ=255)                      tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGtt                                                                                                                 >  2:267663/1‑139 (MQ=255)                      tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGt                                                                                                                  <  1:96289/138‑1 (MQ=255)                      tACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGt                                                                                                                  <  5:218834/138‑1 (MQ=255)                          aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca                                                                                                             <  7:28914/139‑1 (MQ=255)                          aaaaaaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGca                                                                                                             <  3:239162/139‑1 (MQ=255)                              aaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGa                                                                                                         <  7:123308/139‑1 (MQ=255)                              aaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGa                                                                                                         <  7:307662/139‑1 (MQ=255)                              aaCTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGa                                                                                                         <  1:243658/139‑1 (MQ=255)                                   ctgAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGaa                                                                                                    <  4:279685/139‑1 (MQ=255)                                   ctgAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGaa                                                                                                    <  7:87577/139‑1 (MQ=255)                                           ttttGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAat                                                                                             <  3:117051/138‑1 (MQ=255)                                             ttGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAatat                                                                                           <  6:219936/138‑1 (MQ=255)                                                    gagtAGTTTTCTTTTCTTGCTCTCTCATAGTTTAATCACCTCAGCATCATTCTGTACACTTTTCTGAAAATATATACAACCTTTCC‑ATAAAACTTTTTTTGATGTTGTTTGCACAGACTGAACGAAAATATGTAtt                                                                                      <  8:233170/136‑1 (MQ=255)                                                    gagaAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTAtttt                                                                                    <  8:173013/138‑1 (MQ=255)                                                         gTTTTCTTTTCTTCCTCTCTAATTGGTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTgag                                                                              <  6:198529/139‑1 (MQ=255)                                                                    ttcCTCTCTCATTGTTTCATCCCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAATTATGTATTTTTTTGAGAGGAGTGCCa                                                                    >  2:163610/1‑138 (MQ=255)                                                                            tcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGt                                                             <  6:165164/137‑1 (MQ=255)                                                                            tcATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGt                                                             <  4:117120/137‑1 (MQ=255)                                                                            tcATTGTATCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGt                                                             <  5:2668/137‑1 (MQ=255)                                                                              aTTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTAt                                                           <  6:278298/137‑1 (MQ=255)                                                                                  tttCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACa                                                      <  3:186945/138‑1 (MQ=255)                                                                                      atcaCCTCAGCAACAATCTGCACACCATTCAGAAAAAATATCCAACCTCTCC‑CCAAAACTTTTTTAGATGTAGTGTGCACAGACTGAACGAAAATATGTCGTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACa                                                 <  7:80263/139‑1 (MQ=255)                                                                                        caCCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACAtt                                               <  5:321464/139‑1 (MQ=255)                                                                                           cTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCg                                            <  5:252481/139‑1 (MQ=255)                                                                                              aGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCt                                         >  7:86761/1‑139 (MQ=255)                                                                                                cAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTg                                       >  7:289378/1‑139 (MQ=255)                                                                                                  aCATTCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCt                                     <  4:313815/139‑1 (MQ=255)                                                                                                     ttCTGAACACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGaa                                  >  2:284129/1‑139 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              <  2:18435/138‑1 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              <  8:119492/138‑1 (MQ=255)                                                                                                          aaCACTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              <  8:226127/138‑1 (MQ=255)                                                                                                           acacTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATaga                            <  6:141174/139‑1 (MQ=255)                                                                                                           acacTTTTCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATaga                            <  8:86761/139‑1 (MQ=255)                                                                                                               ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACt                        >  3:69003/1‑139 (MQ=255)                                                                                                               ttttCTGAAAATATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACt                        >  3:90920/1‑139 (MQ=255)                                                                                                                      aaaaTATATACAACCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAgg                  <  4:59911/138‑1 (MQ=255)                                                                                                                                cAACCTATCC‑AAAAAACTTTTTTAGATGGAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCg       <  8:45127/139‑1 (MQ=255)                                                                                                                                  aCCTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGAt     >  1:68356/1‑139 (MQ=255)                                                                                                                                   ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              >  2:209090/1‑113 (MQ=255)                                                                                                                                   ccTATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATa                              <  1:209090/113‑1 (MQ=255)                                                                                                                                     tATCC‑AAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGATTta  >  1:317793/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                       TTGCAGGGAACACCCAGCGTTTACGAAAAAACTGCTGAAGTTTTTTGACTTGAGAAGTTTTCTTTTCTTCCTCTCTCATTGTTTCATCACCTCAGCAACATTCTGAACACTTTTCTGAAAATATATACAACCTATCCAAAAAAACTTTTTTAGATGTAGTTTGCACAGACTGAACGAAAATATGTATTTTTTTGAGAGGAGTGCCAAAAACGTATGAACATGACATTGCGTCTTGCTGAACATAGAGACTTGGAGGCTGTTGTGGCGATTTA  >  CP006881/3578329‑3578600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑